Search results for "apte"

Enrichment, isolation and biodegradation potential of psychrotolerant polychlorinated-biphenyl degrading bacteria from the Kongsfjorden (Svalbard Isl…

2016

Persistent organic pollutants (POPs), such as polychlorinated biphenyls (PCBs), have been detected in abiotic Arctic matrices: surface sediments and seawater from coastal areas in the Kongsfjorden were collected and analyzed. Levels of PCBs varied depending on the sampling site. Total PCB concentrations were between 11.63 (site C2W) and 27.69 pg l-1 (site AW). These levels were comparable to those reported previously in lake sediments from the northern Svalbard. The occurrence and biodegradation potential of cold-adapted PCB-oxidizing bacteria in seawater and sediment along the fjord was also evaluated. After enrichment with biphenyl, 246 isolates were obtained with 45 of them that were abl…

AlkAniline-Seq: Profiling of m7 G and m3 C RNA Modifications at Single Nucleotide Resolution.

2018

RNA modifications play essential roles in gene expression regulation. Only seven out of >150 known RNA modifications are detectable transcriptome-wide by deep sequencing. Here we describe a new principle of RNAseq library preparation, which relies on a chemistry based positive enrichment of reads in the resulting libraries, and therefore leads to unprecedented signal-to-noise ratios. The proposed approach eschews conventional RNA sequencing chemistry and rather exploits the generation of abasic sites and subsequent aniline cleavage. The newly generated 5'-phosphates are used as unique entry for ligation of an adapter in library preparation. This positive selection, embodied in the AlkAnilin…

Allergen-Specific Low Zone Tolerance Is Independent of MRP8/14-, TLR4-, TLR7-, and TLR9-Mediated Immune Processes.

2017

Protein-Free Hapten-Carbon Nanotube Constructs Induce the Secondary Immune Response

2017

Carbon nanotubes are novel technological tools with multiple applications. The interaction between such nanoparticles and living organisms is nowadays a matter of keen research by academic and private institutions. In this study, carbon nanotube constructs were investigated as delivery vehicles for immunostimulation and induction of the secondary immune response to a small organic molecule, namely, a hapten. Two types of nanoconstructs were prepared: on one hand, carbon nanotubes carrying a protein bioconjugate of a hapten covalently linked to the carbon surface, and on the other hand, covalent carbon nanotube constructs of the same model chemical compound without the carrier protein. Nanot…

Exosomes as delivery vehicles: a commentary on "Amoxicillin haptenates intracellular proteins that can be transported in exosomes to target cells".

2017

Exosomes are lipoproteic nanosize vesicles of endocytic origin that are released in the extracellular space by several cell types when a multivesicular body fuses with the plasma membrane (1). To date, accumulating evidences highlight the potential of exosomes as messenger in intercellular communication. Indeed, several studies reported the presence of lipids, proteins, metabolites and nucleic acid (DNA, mRNA, microRNA, lncRNA) in exosomes and highlight their functional role in physiological and pathological processes (2).

Ricerche in tema di società questuarie

2019

Intorno al regime delle società di tipo questuario – riconducibili agli schemi teorici della societas universorum quae ex quaestu veniunt – e delle società unius alicuius negotiationis e unius rei, si profusero gli sforzi interpretativi dei giuristi romani, fin dalla fine dell’età repubblicana. La monografia scientifica ripercorre le tracce della individuazione della nozione stessa di quaestus (guadagno), con particolare riferimento alla inclusione al suo interno delle donazioni cd. remuneratorie; ancora, ricostruisce il dibattito giurisprudenziale sorto intorno alla questione se i soci – nella determinazione delle quote – dovessero rispettare una proporzione rispetto ai conferimenti, con u…

Chapter 6 Colon

2009

Abstract The aim of this chapter is to show the key features of adult colon stem cells and provide a useful tool for their isolation, characterization and propagation.

Conception et réalisation d’un dispositif portatif de capteur de rayonnement PAR

2021

National audience; Dans un contexte de changement climatique et de pratiques agricoles tournées vers la préservation desressources, il devient indispensable de suivre précisément la croissance des cultures pour amener une réponserapide en cas de problèmes (stress, bio-agresseurs…). La croissance des plantes repose sur un processusbioénergétique appelé photosynthèse, elle-même stimulée par le rayonnement photosynthétiquement actif (PAR).Combinée au microclimat, cette grandeur devient également un paramètre essentiel dans certains modèles deprédiction. Cependant les coûts ou la complexité des solutions commerciales (capteurs/centrale d’acquisition) sontdes freins pouvant retarder la compréhen…

INCLUSION THROUGH TRAINING AND METHODOLOGY OF ADAPTED PHYSICAL EDUCATION

2023

The theme of practicing sport for people with disabilities goes far beyond the simplification of a healthy lifestyle, but refers to complex aspects of a pedagogical, educational, social, and cultural nature that combine to outline the education and training system of future professionals in this field. This is demonstrated by the growing interest that the question has aroused in recent decades, with the issuing of supranational regulatory provisions that recall the right of all minors, without distinction, to an education capable of harmoniously involving every dimension that constitutes human being, including the education of the body; and this is highlighted by the numerous reviews of int…

L’Adapted Physical Education e l’inclusione

2022

Un’educazione capace di coinvolgere in maniera armonica ogni dimensione che costituisce l’essere umano, ivi compresa l’educazione del corpo richiama l’assunzione di un habitus procedurale in grado di individuare le strategie didattiche e metodologiche più idonee a promuovere processi di inclusione con l’adozione di una pratica fisica e sportiva che sia “per tutti” e “di ciascuno”. Il tema della pratica sportiva per le persone con disabilità, va ben oltre la semplificazione di uno stile di vita sano, ma rimanda ad aspetti complessi di natura pedagogica, educativa, sociale e culturale che concorrono a delineare il sistema di istruzione e di formazione dei futuri professionisti in tale ambito.…