Search results for "asma"

Characterization of antihistamine–human serum protein interactions by capillary electrophoresis

2007

An important topic in the drug discovery and development process is the role of drug binding to plasma proteins. In this paper the characterization of the interaction between antihistamines (cationic drugs) towards human serum albumin (HSA) and alpha(1)-acid glycoprotein (AGP) under physiological conditions by capillary electrophoresis-frontal analysis is presented. Furthermore, the binding of these drugs to all plasma proteins is evaluated by using ultrafiltration and capillary electrophoresis. Antihistamines present a wide-ranging behaviour with respect to their affinities towards plasma proteins. Orphenadrine, phenindamine, tripelenamine and tripolidine principally bind to HSA; carbinoxa…

Infrared biospectroscopy for a fast qualitative evaluation of sample preparation in metabolomics.

2014

Liquid chromatography-mass spectrometry (LC-MS) has been increasingly used in biomedicine to study the dynamic metabolomic responses of biological systems under different physiological or pathological conditions. To obtain an integrated snapshot of the system, metabolomic methods in biomedicine typically analyze biofluids (e.g. plasma) that require clean-up before being injected into LC-MS systems. However, high resolution LC-MS is costly in terms of resources required for sample and data analysis and care must be taken to prevent chemical (e.g. ion suppression) or statistical artifacts. Because of that, the effect of sample preparation on the metabolomic profile during metabolomic method d…

Determination of benperidol and its reduced metabolite in human plasma by high-performance liquid chromatography and electrochemical detection.

1991

An isocratic high-performance liquid chromatographic method with electrochemical detection for the quantification of benperidol and its suggested reduced metabolite TVX Q 5402 in human plasma is described. The method included a two-step solid-phase extraction on reversed-phase and cation-exchange material, followed by separation on a cyanopropyl silica gel column (5 microns; 250 mm x 4.6 mm I.D.). The eluent was 0.15 M acetate buffer (pH 4.7) containing 25% acetonitrile (w/w). Spiperone served as internal standard. The inclusion of the cation-exchange step provided sample purity higher than those achieved with other methods. After extraction of 1 ml of plasma, concentrations as low as 0.5 n…

Peptides analysis in blood plasma using on-line system of supported liquid membrane and high-performance liquid chromatography

2005

The potential of using supported liquid membrane (SLM) technique, combined with reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated for the determination of peptides in human blood plasma. The peptides studied were (DL)Leu(DL)Phe, MetLeuPhe, GlyLeuTyr and ValGluProlleProTyr. The carrier (Aliquat 336) was incorporated in membrane phase in order to facilitate the transport of investigated peptides. After extraction, the analyte-enriched acceptor phase was directly injected into an HPLC system for analysis. With SLM, high selectivity and efficiency were achieved for extraction of peptides in aqueous solutions. Lower extraction efficiency was obtained in plasma…

Development of a species-unspecific isotope dilution GC-ICPMS method for possible routine quantification of sulfur species in petroleum products.

2008

Inductively coupled plasma mass spectrometry (ICPMS) hyphenated with capillary gas chromatography was applied for sulfur multispecies determination in petroleum products by species-unspecific isotope dilution mass spectrometry (IDMS). To guarantee a stable and continuous addition of the spike into the GC-ICPMS system, a special dosing unit was designed and synthesis of a (34)S-labeled dimethyldisulfide spike from (34)S-enriched elemental sulfur in the milligram range was developed. The sample was mixed with an internal standard for spike mass flow calibration. From the mass flow chromatogram obtained by species-unspecific GC-ICP-IDMS, determination of all separated sulfur species and of the…

Determination of chromium in foods by flame atomic absorption and inductively coupled plasma spectrometry

1989

Statistical characterisation of heavy metal contents inParacentrotus lividusfrom Mediterranean Sea

2014

This work focuses on the estimation of Hg, As, Cr, Ni, Cu, V, Cd and Pb by inductively coupled plasma mass spectrometry in 135 adult specimens of Paracentrotus lividus collected in different coastal areas of Sicily (Gela, Punta Secca, Ragusa (RG), Siracusa, Priolo, Catania, Messina, Milazzo, Brolo and Filicudi), in order to monitor the Mediterranean marine ecosystem by use of sea urchin as bioindicator. Moreover, the paper deals with the statistical classification of the tested samples according to the sampling area based on metal concentrations. The descriptive statistics findings were obtained and, a starting multivariate matrix was built. Data-sets were subjected to Kruskal-Wallis test t…

Estudio sobre la cicatrización de la zona donante de injerto de piel parcial en pacientes quemados tras aplicación de plasma rico en plaquetas o plas…

2019

La técnica más empleada para la restauración de la barrera cutánea en los pacientes quemados es el injerto de piel autólogo de espesor parcial. Su obtención conlleva generar un área cruenta a nivel de otra localización cutánea del mismo paciente, conocida como zona donante. Esta área cura generalmente en un período de entre 7 y 14 días mediante un proceso de cicatrización dirigida. Cuando existen grandes defectos que requieren cobertura, como es el caso de los grandes quemados en los que la extensión de la quemadura limita la disponibilidad de área donante, pueden ser necesarias varias tomas de la misma zona. Por lo tanto, la opción terapéutica global en este tipo de pacientes va a depender…

Detailed study of SNR G306.3–0.9 using XMM-Newton and Chandra observations

2016

Aims. We aim to study the spatial distribution of the physical and chemical properties of the X-ray emitting plasma of the supernova remnant (SNR) G306.3-0.9 in detail to obtain constraints on its ionization stage, the progenitor supernova explosion, and the age of the remnant. Methods. We used combined data from XMM-Newton and Chandra observatories to study the X-ray morphology of G306.3-0.9 in detail. A spatially resolved spectral analysis was used to obtain physical and geometrical parameters of different regions of the remnant. Spitzer infrared observations, available in the archive, were also used to constrain the progenitor supernova and study the environment in which the remnant evol…

Role of Seroalbumin in the Cytotoxicity of cis-Dichloro Pt(II) Complexes with (N^N)-Donor Ligands Bearing Functionalized Tails

2018

Given the potent anticancer properties of cisdiamminedichloroplatinum( II) and knowing its mode of action, we synthesized four new cis-[PtCl2(N^N)] organoplatinum complexes, two with N-substituted pbi ligands (pbiR = 1-R-2-(2-pyridyl)benzimidazole) (namely, 1 and 2) and two more with 4,4′-disubstituted bpy ligands (bpy = 2,2′-bipyridine) (namely, 3 and 4). We explored their cytotoxicity and ability to bind to deoxyguanosine monophosphate (dGMP), DNA, and albumin models. By 1H NMR and UV−vis spectroscopies, circular dichroism, agarose gel electrophoresis, differential scanning calorimetry measurements, and density functional theory calculations, we verified that only 3 can form aquacomplex s…