Search results for "attachment"

Changes in Periodontal Parameters and C-Reactive Protein After Pregnancy.

2016

This study assesses hormonal, inflammatory, and periodontal changes in pregnant women and postpartum in the absence of periodontal treatment, and seeks to determine any correlations among these parameters.A longitudinal, observational study of 117 pregnant women (aged 23 to 42 years) was undertaken in a private gynecologic center between weeks 32 and 35 of pregnancy and 6 to 8 weeks after delivery. Levels of progesterone and C-reactive protein (CRP) in plasma were determined, as well as periodontal indices, including: 1) plaque index (PI); 2) bleeding on probing (BOP); 3) probing depth (PD); and 4) clinical attachment level (CAL).Postpartum progesterone and CRP declined sharply from 90.85 ±…

The dark side of romantic relationships: Aggression in adolescent couples and links to attachment

2015

Item does not contain fulltext This study focuses on romantic relationships from the perspective of both partners. This dyadic approach was chosen to account for the fact that both partners may differently contribute to the escalation of aggression. In a sample of 194 romantic partner dyads, differences between female and male partners׳ reports of aggression (relational and physical) and measures of attachment security and jealousy were assessed. A hierarchical cluster analysis identified five distinct subgroups of dyads with mutually aggressive or one-sided aggressive dyad. Of note were dyads with aggressive females and self-silencing males. The mutually aggressive couples showed the least…

Noroviral P-Particles as an In Vitro Model to Assess the Interactions of Noroviruses with Probiotics

2014

Noroviruses (NoVs) are the main etiologic agents of acute epidemic gastroenteritis and probiotic bacteria have been reported to exert a positive effect on viral diarrhea. The protruding (P) domain from NoVs VP1 capsid protein has the ability to assemble into the so-called P-particles, which retain the binding ability to host receptors. We purified the P-domains from NoVs genotypes GI.1 and GII.4 as 6X(His)-tagged proteins and determined that, similar to native domains, they were structured into P-particles that were functional in the recognition of the specific glycoconjugated receptors, as established by surface plasmon resonance experiments. We showed that several lactic acid bacteria (pr…

Morphogenetic (Mucin Expression) as Well as Potential Anti-Corona Viral Activity of the Marine Secondary Metabolite Polyphosphate on A549 Cells

2020

The mucus layer of the nasopharynx and bronchial epithelium has a barrier function against inhaled pathogens such as the coronavirus SARS-CoV-2. We recently found that inorganic polyphosphate (polyP), a physiological, metabolic energy (ATP)-providing polymer released from blood platelets, blocks the binding of the receptor binding domain (RBD) to the cellular ACE2 receptor in vitro. PolyP is a marine natural product and is abundantly present in marine bacteria. Now, we have approached the in vivo situation by studying the effect of polyP on the human alveolar basal epithelial A549 cells in a mucus-like mucin environment. These cells express mucins as well as the ectoenzymes alkaline phospha…

Podstawowe samowartościowanie - walidacja polskiej wersji skali do pomiaru Core Self-Evaluations

2017

Praca przedstawia walidację polskiej wersji skali do pomiaru podstawowego samowartościowania (Core Self-Evaluations – CSES; Judge, Erez, Bono i Thorensen, 2003). Podstawowe samowartościowanie to poglądy danej osoby, dotyczące jej skuteczności, zdolności, wiary we własne siły i pozytywnego nastawienia do siebie i świata. Koncepcja skali jest konstruktem opartym o indywidualne zmienne dyspozycyjne, często badane w kontekście organizacyjnym. Są to: samoocena, wewnętrzne umiejscowienie kontroli, zgeneralizowana samoskuteczność oraz pozytywna afektywność. Na podstawie danych z pięciu badań przeprowadzonych w latach 2014-2016, na łącznie n=3207 osób, w większości pracujących, udało się potwierdzi…

Examining the Mediating Role of Attachment Style in the Relationship between Childhood Maltreatment and Borderline Personality Disorder in Adolescent…

2022

Reply to "Heparan Sulfate in Baculovirus Binding and Entry of Mammalian Cells"

2014

(1), we investigated the interaction ofbaculovirus and mammalian cell surface heparan sulfate pro-teoglycans (HSPG). The data show that baculovirus requiresHSPG sulfation, particularly N- and 6-O-sulfation, to bind andtransduce mammalian cells. We also show that baculovirus asso-ciates specifically with syndecan-1 (SDC-1) but not with othersyndecans or glypicans.As discussed in the article, HS has previously been shown to beinvolved in glycoprotein 64 (gp64)-mediated baculovirus bindingonto mammalian cells. Heparin and heparinase I and II treatmentof cells have also been shown to prevent the virus binding (2, 3).The role of HS in baculovirus entry was further studied in ourarticle (1). Bindi…

Association between chronic dental infection and acute myocardial infarction.

2008

Introduction: In patients with cardiovascular diseases several risk factors such as high blood pressure, diabetes, smoking and drinking habits, genetic disposition, and chronic inflammation must be considered. The aim of this study was to investigate whether there is a correlation between dental origin infections and the presence of an acutemyocardialinfarction(AMI).Methods:Atotalof125 patients who had experienced a myocardial infarction and 125healthyindividualswereincludedinthisstudy.Theoral examination was carried out following the consent of the ethics committee and the National Board for Radiation Protection and included the number of teeth, endodontically treated teeth, periodontal sc…

Soluble N-ethylmaleimide-sensitive-factor attachment protein and N-ethylmaleimide-insensitive factors are required for Ca2+-stimulated exocytosis of …

1996

Ca2+ stimulates exocytosis in permeabilized insulin-secreting cells. To investigate the putative cytosolic components involved in the Ca2+ response, HIT-T15 cells (a pancreatic B-cell line) were permeabilized with streptolysin-O, a procedure that allows rapid exchange of soluble components including macromolecules. We found that in this cell preparation the secretory response to Ca2+ but not to guanosine 5'-[gamma-thio]triphosphate was lost as a function of time and could be restored by rat brain cytosol in a concentration-dependent manner. Reconstitutive activity of rat brain cytosol was found in a high-molecular-mass heat-labile partially N-ethylmaleimide(NEM)-sensitive fraction. The NEM-…

Chromatin Domains and Regulation of Transcription

2007

Compartmentalization and compaction of DNA in the nucleus is the characteristic feature of eukaryotic cells. A fully extended DNA molecule has to be compacted 100,000 times to fit within the nucleus. At the same time it is critical that various DNA regions remain accessible for interaction with regulatory factors and transcription/replication factories. This puzzle is solved at the level of DNA packaging in chromatin that occurs in several steps: rolling of DNA onto nucleosomes, compaction of nucleosome fiber with formation of the so-called 30 nm fiber, and folding of the latter into the giant (50-200 kbp) loops, fixed onto the protein skeleton, the nuclear matrix. The general assumption is…