Search results for "bacteri"

Nitrogen-fixing cushion Astragalus siculus modulates soil fertility, microclimate, plant facilitation, bacterial and fungal microbiota along an eleva…

2023

Question: Cushion-forming vascular plants are adapted to alpine environments and act as ecosystem engineers by moderating microclimate and creating a fertility island under their canopy. Most of the available studies on cushion ecology have been devoted to plant facilitation, but none used a holistic approach that considers the response of co-existing plant species with other key ecosystem players. Here, we quantified the biogenic effect and facilitation capacity of Astragalus siculus, a spiny nitrogen-fixing cushion plant, and test the hypothesis that the facilitation effect would increase with elevation as stress gradient.Location: Mount Etna, the highest active volcano in the Mediterrane…

bbFISH-ing in the sonication fluid

2019

Abstract By 2030, the annual number of combined total hip and knee arthroplasty is estimated to reach 3.5 to 4 million in the US alone. In the context of a constant increase of the number of primary and revision total hip and knee arthroplasty, an increased risk of complication is expected. Prosthetic joint infections (PJIs) represent major cause of healthcare expenditure and morbidity. PJI still remain the most common and feared arthroplasty complication. A rapid and correct diagnosis of infection is decisive for a correct therapeutical management. In this setting, the Academic Emergency Hospital Sibiu adopted and implemented, with the beginning of September 2016, a new strategy for the di…

The evolution of temperature tolerance and invasiveness in a fluctuating thermal environment

2016

The consequences of the climate change on species are still uncertain, despite of intensive research. Currently, rising temperature is not the only concern, since the climate change scenarios also predict increases in the amount of disturbances, such as storms, floods, and thermal fluctuations. Disturbances have also been shown to affect species’ evolution, for example by selecting for traits that are advantageous in fluctuating environments but are also facilitating invasiveness. In this thesis, I study the consequences of evolving in a fluctuating thermal environment by utilizing bacterial microcosms. First I tested the effects of fluctuating vs. constant temperature on the evolution of t…

Discovery of a new class of sortase a transpeptidase inhibitors to tackle gram-positive pathogens: 2-(2-phenylhydrazinylidene)alkanoic acids and rela…

2016

A FRET-based random screening assay was used to generate hit compounds as sortase A inhibitors that allowed us to identify ethyl 3-oxo-2-(2-phenylhydrazinylidene)butanoate as an example of a new class of sortase A inhibitors. Other analogues were generated by changing the ethoxycarbonyl function for a carboxy, cyano or amide group, or introducing substituents in the phenyl ring of the ester and acid derivatives. The most active derivative found was 3-oxo-2-(2-(3,4dichlorophenyl)hydrazinylidene)butanoic acid (2b), showing an IC50 value of 50 µM. For a preliminary assessment of their antivirulence properties the new derivatives were tested for their antibiofilm activity. The most active compo…

Preliminary Investigation of Biogenic Amines in Type I Sourdoughs Produced at Home and Bakery Level

2022

During a survey for isolating sourdough lactic acid bacteria (LAB), 20 dough samples produced at the bakery level (BL) or home-made (HM) were collected. An enzyme-based colorimetric method revealed a total biogenic amines (BAs) concentration in the range 41.4–251.8 ppm for six (three BL and three HM) sourdoughs characterised by unpleasant odours. Eight BAs generally investigated in foods were identified and quantified from these six samples by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS). Only one HM sample contained almost all analysed BAs. Tryptamine was exclusively detected in HM sourdoughs (0.71–24.1 ppm). Putrescine, tryptamine, …

Approccio multiplo per l’indagine del microbiota dominante negli impasti acidi tradizionali siciliani

2011

Microbial resources and sparkling wine differentiation : state of the arts

2022

Consumers’ increasing interest in sparkling wine has enhanced the global market’s demand. The pro-technological yeasts strains selected for the formulation of microbial starter cultures are a fundamental parameter for exalting the quality and safety of the final product. Nowadays, the management of the employed microbial resource is highly requested by stakeholders, because of the increasing economic importance of this oenological sector. Here, we report an overview of the production processes of sparkling wine and the main characterisation criteria to select Saccharomyces and non-Saccharomyces strains appropriate for the preparation of commercial starter cultures dedicated to the primary a…

Sex and sexual ornamentation associated with survival of the cyprinid fish, Rutilus rutilus, under disease stress

2013

By conveying information of disease resistance, sexual signals may be used as cues for adaptive mate choice. Here we report observations on survival of laboratory-maintained, wild-collected, sexually mature, ready-to-spawn cyprinid fish, Rutilus rutilus (roach), under accidental epidemic attributed to Flavobacterium psychrophilum, diagnosed using species-specific PCR. The fish were maintained in a single tank. During the 27 days observation period, both the proportion of fish surviving the infection as well as the length-adjusted mean survival time of the fish that died was the highest among the high-ornamented males with large breeding tubercles, intermediary among the low-ornamented males…

Latgales reģionā cirkulējošo Mycobacterium tuberculosis genotipu raksturojums

2019

Tuberkuloze ir pasaulē izplatīta infekcijas slimība, kuras izraisītājas ir M. tuberculosis kompleksā ietilpstošās mikobaktērijas. Darbs tika veikts, lai noteiktu un raksturotu Latgales reģionā cirkulējošo M. tuberculosis genotipus. Darbā ir izmantotas molekulāras genotipēšanas metodes: starpfragmentu oligonukleotīdu genotipēšanas metode, kas ir balstīta uz polimerāzes ķēdes reakciju un tiešā atkārtojuma (DR) reģiona amplificēšanu; restrikcijas fragmentu garuma polimorfisma analīzes metode, kur M. tuberculosis genotips tiek noteikts pēc insercijas sekvenču IS6110 kopiju skaita un izvietojuma atšķirībām hromosomā. Rezultātos tiek atspoguļots, kādas līnijas un apakšlīnijas atrastas Latgales re…

Concentración mínima inhibitoria (CMI) de vancomicina para Staphylococcus aureus y riesgo de bacteriemia complicada

2019

Staphylococcus aureus (S. aureus) se encuentra ampliamente distribuido por todo el mundo, comportándose tanto como patógeno como comensal. Se estima que entre un 25-50% de personas sanas puede colonizarse de manera persistente o transitoria (Longo, 2012). Este microorganismo constituye una de las principales causas de infecciones nosocomiales, siendo una de las primeras causas de bacteriemias primarias (Rigby and DeLeo, 2012) y encabezando la lista de los productores de infecciones de piel y tejidos blandos, heridas quirúrgicas, endocarditis y sepsis relacionada con catéter (Seidl et al., 2011). La bacteriemia se define como la presencia de bacterias viables en sangre; ésta puede o no estar…