Search results for "bacteri"

showing 10 items of 5466 documents

Abundance of narG , nirS , nirK , and nosZ Genes of Denitrifying Bacteria during Primary Successions of a Glacier Foreland

2006

ABSTRACT Quantitative PCR of denitrification genes encoding the nitrate, nitrite, and nitrous oxide reductases was used to study denitrifiers across a glacier foreland. Environmental samples collected at different distances from a receding glacier contained amounts of 16S rRNA target molecules ranging from 4.9 × 10 5 to 8.9 × 10 5 copies per nanogram of DNA but smaller amounts of narG , nirK , and nosZ target molecules. Thus, numbers of narG , nirK , nirS , and nosZ copies per nanogram of DNA ranged from 2.1 × 10 3 to 2.6 × 10 4 , 7.4 × 10 2 to 1.4 × 10 3 , 2.5 × 10 2 to 6.4 × 10 3 , and 1.2 × 10 3 to 5.5 × 10 3 , respectively. The densities of 16S rRNA genes per gram of soil increased with…

ALPINE DEVELOPMENTDNA BacterialglacierNitrite ReductasesDenitrificationNitrogenDenitrification pathwayDIVERSITYBiologyNitrate ReductasePolymerase Chain ReactionApplied Microbiology and BiotechnologyCOLONIZATIONMicrobial EcologyDenitrifying bacteriaRNA Ribosomal 16SBotanyIce CoverMICROBIAL COMMUNITIESGlacier forelandPoaPrimary successionEcosystemSoil Microbiology[SDV.EE]Life Sciences [q-bio]/Ecology environmentRhizosphereBacteriaBase SequenceEcologyRHIZOSPHEREQUANTIFICATIONNitrite reductaseSOILSRNA BacterialGenes BacterialAustriaOxidoreductasesSoil microbiologyFood ScienceBiotechnologyApplied and Environmental Microbiology
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Key features of bacterial communities in Southern Europe soils vulnerable to desertification

2019

Mediterranean soils are experiencing increasingly negative effects of climate change due to drought and extreme weather phenomena that negatively affect agriculture, already suffering social and economic constraints. The LIFE project DESERT-ADAPT - Preparing desertification areas for increased climate change - (http://www.desert-adapt.it/index.php/en/) focuses on agricultural adaptation measures aimed at reverting ongoing desertification trends, while improving the socioeconomic conditions of farmers. The soil microbiota is recognized as key player in both diversity and productivity of terrestrial ecosystems and it is mandatory to include its role in sustainable agronomic management. In ord…

ARISAbacterial diversitysoil microbiotaSettore BIO/19 - Microbiologia GeneraleDesertification
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Safety and immunomodulatory effects of three probiotic strains isolated from the feces of breast-fed infants in healthy adults: SETOPROB study.

2013

We previously described the isolation and characterization of three probiotic strains from the feces of exclusively breast-fed newborn infants: Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036. These strains were shown to adhere to intestinal mucus in vitro, to be sensitive to antibiotics and to resist biliary salts and low pH. In the present study, a multicenter, randomized, double-blind, placebo-controlled trial with 100 healthy volunteers in three Spanish cities was carried out to evaluate the tolerance, safety, gut colonization and immunomodulatory effects of these three probiotics. Volunteers underwent a 15-day washout perio…

ARN Bacterianoved/biology.organism_classification_rank.speciesPhysiologylcsh:Medicine:Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Environment::Ecosystem::Biodiversity::Biota::Microbiota [Medical Subject Headings]law.inventionFecesProbioticAntibioticslawLactobacillus:Phenomena and Processes::Physiological Phenomena::Nutritional Physiological Phenomena::Child Nutritional Physiological Phenomena::Infant Nutritional Physiological Phenomena::Breast Feeding [Medical Subject Headings]lcsh:Science:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::RNA::RNA Bacterial [Medical Subject Headings]BifidobacteriumMultidisciplinaryBifidobacterium brevebiologyLacticaseibacillus rhamnosusMicrobiotaHibridación in SituInterleukin-10:Organisms::Bacteria::Gram-Positive Bacteria::Lactobacillales::Lactobacillaceae::Lactobacillus [Medical Subject Headings]Breast FeedingBloodCytokinesFemaleResearch ArticleAdult:Anatomy::Fluids and Secretions::Feces [Medical Subject Headings]Lactobacillus paracasei:Organisms::Bacteria::Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Bacteria::Gram-Positive Endospore-Forming Rods::Clostridium::Clostridium difficile [Medical Subject Headings]Microbiology:Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::Nucleic Acid Probes::Oligonucleotide Probes [Medical Subject Headings]Double-Blind MethodLactobacillus rhamnosusHumansImmunologic Factors:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Histocytological Preparation Techniques::Staining and Labeling::In Situ Hybridization [Medical Subject Headings]FecesSafety studiesved/biologyProbioticslcsh:RClostridium difficile:Organisms::Bacteria::Gram-Positive Bacteria::Actinobacteria::Bifidobacterium [Medical Subject Headings]biology.organism_classificationImmunoglobulin ALactobacilluslcsh:QInterleukin-4BifidobacteriumBreast feedingSondas de OligonucleótidosPLoS ONE
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Genome and phenotype microarray analyses of rhodococcus sp. BCP1 and rhodococcus opacus R7: Genetic determinants and metabolic abilities with environ…

2015

In this paper comparative genome and phenotype microarray analyses of Rhodococcus sp. BCP1 and Rhodococcus opacus R7 were performed. Rhodococcus sp. BCP1 was selected for its ability to grow on short-chain n-alkanes and R. opacus R7 was isolated for its ability to grow on naphthalene and on o-xylene. Results of genome comparison, includ- ing BCP1, R7, along with other Rhodococcus reference strains, showed that at least 30% of the genome of each strain presented unique sequences and only 50% of the predicted proteome was shared. To associate genomic features with metabolic capabilities of BCP1 and R7 strains, hundreds of different growth conditions were tested through Phenotype Microarray, b…

AROMATIC-COMPOUNDS; GENUS RHODOCOCCUS; HIGH-THROUGHPUT; PATHWAY; DEGRADATION; BIODEGRADATION; EQUI; PERFORMANCE; CATABOLISMGenomics RhodococcusGene predictionBacterial Proteinlcsh:MedicineBiologyGenomeXenobioticsRhodococcus opacusBacterial ProteinsRhodococcuslcsh:ScienceGenePhylogenyGeneticsComparative genomicsMultidisciplinarylcsh:RMetabolic Networks and PathwayPhenotype microarrayHigh-Throughput Nucleotide SequencingRhodococcus sp. BCP1 Rhodococcus opacus R7Genome analysisGene Expression Regulation BacterialGenomicsSequence Analysis DNAbiology.organism_classificationBIO/19 - MICROBIOLOGIA GENERALEBiodegradation EnvironmentalPhenotypeProteomeGenomiclcsh:QPhenotype MicroarrayRhodococcusMetabolic Networks and PathwaysRhodococcuhydrocarbon degradationResearch Article
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Inducible ASABF-Type Antimicrobial Peptide from the Sponge Suberites domuncula: Microbicidal and Hemolytic Activity in Vitro and Toxic Effect on Moll…

2011

Since sponges, as typical filter-feeders, are exposed to a high load of attacking prokaryotic and eukaryotic organisms, they are armed with a wide arsenal of antimicrobial/cytostatic low-molecular-weight, non-proteinaceous bioactive compounds. Here we present the first sponge agent belonging to the group of ASABF-type antimicrobial peptides. The ASABF gene was identified and cloned from the demospongeSuberites domuncula. The mature peptide, with a length of 64 aa residues has a predicted pI of 9.24, and comprises the characteristic CSαβ structural motif. Consequently, the S. domuncula ASABF shares high similarity with the nematode ASABFs ; it is distantly related to the defensins. The recom…

ASABFAntimicrobial peptidesGastropodaMolecular Sequence DataPharmaceutical SciencePeptideMicrobial Sensitivity TestsGram-Positive BacteriaReal-Time Polymerase Chain ReactionArticleMicrobiology03 medical and health sciencesantimicrobial peptidesAnti-Infective AgentsSequence Analysis ProteinDrug DiscoveryAnimalsBittium sp.Structural motiflcsh:QH301-705.5Pharmacology Toxicology and Pharmaceutics (miscellaneous)spongesPhylogeny030304 developmental biologychemistry.chemical_classification0303 health sciencesbiology030306 microbiologyEffectorHemolytic AgentsapoptosisGeologyBittium spsponges; <em>Suberites domuncula</em>; ASABF; antimicrobial peptides; apoptosis; <em>Bittium</em> sp.biology.organism_classificationSuberites domunculasponges ; Suberites domuncula ; ASABF ; antimicrobial peptides ; apoptosis ; Bittium sp.Recombinant ProteinsSuberites domunculaSpongeEnzymelcsh:Biology (General)chemistryMolluscaSuberitesSuberitesAntimicrobial Cationic PeptidesMarine Drugs
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CitA/CitB Two-Component System Regulating Citrate Fermentation in Escherichia coli and Its Relation to the DcuS/DcuR System In Vivo

2011

ABSTRACT Citrate fermentation by Escherichia coli requires the function of the citrate/succinate antiporter CitT ( citT gene) and of citrate lyase ( citCDEFXG genes). Earlier experiments suggested that the two-component system CitA/CitB, consisting of the membrane-bound sensor kinase CitA and the response regulator CitB, stimulates the expression of the genes in the presence of citrate, similarly to CitA/CitB of Klebsiella pneumoniae . In this study, the expression of a chromosomal citC-lacZ gene fusion was shown to depend on CitA/CitB and citrate. CitA/CitB is related to the DcuS/DcuR two-component system which induces the expression of genes for fumarate respiration in response to C 4 -di…

ATP citrate lyaseOperonBiologymedicine.disease_causeMicrobiologyCitric AcidFusion geneGene clusterEscherichia colimedicinePromoter Regions GeneticMolecular BiologyEscherichia coliEscherichia coli ProteinsPromoterGene Expression Regulation BacterialArticlesMolecular biologyTwo-component regulatory systemDNA-Binding ProteinsResponse regulatorBiochemistryFermentationProtein KinasesProtein BindingTranscription FactorsJournal of Bacteriology
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Genetic organization of the citCDEF locus and identification of mae and clyR genes from Leuconostoc mesenteroides.

1999

ABSTRACT In this paper, we describe two open reading frames coding for a NAD-dependent malic enzyme ( mae ) and a putative regulatory protein ( clyR ) found in the upstream region of citCDEFG of Leuconostoc mesenteroides subsp. cremoris 195. The transcriptional analysis of the citrate lyase locus revealed one polycistronic mRNA covering the mae and citCDEF genes. This transcript was detected only on RNA prepared from cells grown in the presence of citrate. Primer extension experiments suggest that clyR and the citrate lyase operon are expressed from a bidirectional A-T-rich promoter region located between mae and clyR.

ATP citrate lyaseTranscription GeneticOperonMolecular Sequence DataMalatesLocus (genetics)Genetics and Molecular BiologyBiologyMicrobiologyPrimer extensionCitric AcidOpen Reading FramesBacterial ProteinsMalate DehydrogenaseMultienzyme ComplexesLeuconostocAmino Acid SequenceMolecular BiologyGeneGeneticsBase SequenceOxo-Acid-LyasesPromoterGene Expression Regulation BacterialSequence Analysis DNAbiology.organism_classificationMolecular biologyLeuconostoc mesenteroidesGenes BacterialSequence AlignmentLeuconostocJournal of bacteriology
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Acid sensitivity of neomycin-resistant mutants ofOenococcus oeni: a relationship between reduction of ATPase activity and lack of malolactic activity

1999

Mutants of Oenococcus oeni were isolated as spontaneous neomycin-resistant mutants. Three of these mutants harbored a significantly reduced ATPase activity that represented 50% of that of the wild-type strain. Their growth rates were also impaired at pH 5.3 (46-86% of the wild-type level). However, the profiles of sugar consumption appeared identical to those of the parental strain. At pH 3.2, all the mutant strains failed to grow and a drastic decrease in viability was observed after an acid shock. Surprisingly, all the isolated mutants were devoid of malolactic activity. These results suggest that the ATPase and malolactic activities of O. oeni are linked to each other and play a crucial …

ATPaseMutantMalatesMicrobiologyMicrobiologyGeneticsmedicineMalolactic fermentationLactic AcidMolecular BiologyHeat-Shock ProteinsOenococcus oeniAdenosine Triphosphataseschemistry.chemical_classificationbiologyStrain (chemistry)Drug Resistance MicrobialNeomycinNeomycinHydrogen-Ion Concentrationbiology.organism_classificationAnti-Bacterial AgentsGram-Positive CocciEnzymeBiochemistrychemistrybiology.proteinHeat-Shock ResponseLeuconostocBacteriamedicine.drugFEMS Microbiology Letters
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Intestinal tuberculosis in a child living in a country with a low incidence of tuberculosis : a case report

2014

Background: Relatively common in adults, intestinal tuberculosis is considered rare in children and adolescents. The protean manifestations of intestinal tuberculosis mean that the diagnosis is often delayed (sometimes even for years), thus leading to increased mortality and unnecessary surgery. The main diagnostic dilemma is to differentiate intestinal tuberculosis and Crohn’s disease because a misdiagnosis can have dramatic consequences. Case presentation: A 13-year-old Caucasian, Italian female adolescent attended the Emergency Department complaining of abdominal pain, a fever of up to 38°C, night sweats, diarrhea with blood in stool, and a weight loss of about three kilograms over the p…

Abdominal painBiopsymedicine.medical_treatmentAntitubercular AgentsCase ReportInflammatory bowel diseaseGastroenterologySettore MED/38 - Pediatria Generale E SpecialisticaCrohn DiseaseLaparotomyWhole Body ImagingMedicine(all)biologymedicine.diagnostic_testIleal DiseasesIncidenceGeneral MedicineEmerging infectionsTreatment OutcomeItalyIntestinal tuberculosisAbdominal ultrasonographyDrug Therapy CombinationFemalemedicine.symptommedicine.medical_specialtyMiliary tuberculosisTuberculosisAdolescentGeneral Biochemistry Genetics and Molecular BiologyDiagnosis DifferentialMycobacterium tuberculosisPredictive Value of TestsInternal medicineGastrointestinal infectionsmedicineHumansTuberculosisDiagnostic ErrorsEmerging infections Gastrointestinal infections Intestinal tuberculosis Mycobacterium tuberculosis TuberculosisTuberculosis MiliaryBiochemistry Genetics and Molecular Biology(all)business.industryMycobacterium tuberculosisAbdominal distensionmedicine.diseasebiology.organism_classificationSurgeryTuberculosis GastrointestinalTomography X-Ray Computedbusiness
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Bacterial community response to changes in a tri-trophic cascade during a whole-lake fish manipulation

2015

Microbial communities play a key role in biogeochemical processes by degrading organic material and recycling nutrients, but can also be important food sources for upper trophic levels. Trophic cascades might modify microbial communities either directly via grazing or indirectly by inducing changes in other biotic or in abiotic factors (e.g., nutrients). We studied the effects of a tri-trophic cascade on microbial communities during a whole-lake manipulation in which European perch (Perca fluviatilis) were added to a naturally fishless lake divided experimentally into two basins. We measured environmental parameters (oxygen, temperature, and nutrients) and zooplankton biomass and studied th…

Abiotic componentnext generation sequencingBiomass (ecology)anaerobic microbesEcologyfungiboreal lakesBiologyZooplanktonFood chainbacterial community compositionNutrientMicrobial population biologytrophic cascadesTrophic cascadetop-down vs. bottom-up controlEcology Evolution Behavior and SystematicsTrophic level
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