Search results for "biotin"
showing 10 items of 127 documents
Ligand-receptor interactions directly measured with the surface forces apparatus
1991
Ligand-receptor interactions give rise to very strong bonds due to perfect geometrical fit. Using the Surface Forces Apparatus we have studied the interactions between membrane-bound biotin ligands and streptavidin receptors. We find an unusually strong short-range binding force associated with equally specific molecular rearrangements-both qualitatively and quantitatively unlike anything previously measured.
Protein-Membrane Interaction
2012
In the following chapter I present a nanosized and addressable protein sensor platform based on membrane coated plasmonic particles. I show unequivocally the covering of gold nanorods with lipid bilayers and thereafter the subsequent detection of streptavidin binding to biotin moities in the lipids. The binding of the streptavidin on the membrane covered gold nanorods is detected by monitoring the spectral shift by fast single particle spectroscopy (fastSPS) on many particles in parallel.
Functionalized lipid tubules as tools for helical crystallization of proteins
1997
The development of functional supramolecular devices built by self-assembly of elementary molecules and with bioactive properties arouses considerable interest in the field of nanotechnology and new materials. We report here the formation of a new class of lipid tubules exhibiting both properties of molecular recognition and crystal formation for the protein streptavidin. These lipid tubules, made of biotin-containing dioctadecylamine molecules, are straight hollow cylinders with a constant diameter of 27 nm and variable length up to several micrometers. They are unilamellar with an inner diameter of about 16 nm, as shown by cryoelectron microscopy. Streptavidin binds to the biotinylated tu…
Preservation of vitamins content in Cuccìa using an innovative method of processing
2019
Cuccìa is a traditional Sicilian food prepared by boiling whole durum wheat kernels, in water, for many hours. This process destroys the vitamins E and B contents of crude kernels. It was rated a method to prepare the Cuccìa, preserving the vitamin content. Four varieties of durum wheat were processed comparing the traditional cooking method (TR-boiling for 5/6 hours), and an innovative one (IN-grains scarification, germination, and cooking at 50 °C for 2 hours). On soups obtained the content of biotin, niacin and α-amylase activity were determined. ANOVA showed the cooking method influences biotin and niacin content having values from 0.56 and 0.72 ng ml−1 (raw grain) and values close to 0…
Promotion of osteogenic cell response using quasicovalent immobilized fibronectin on titanium surfaces: introduction of a novel biomimetic layer syst…
2012
Purpose Despite the undeniable potential of cell adhesion molecules such as fibronectin to support osteogenic cell responses and consecutive dental implant healing, the most beneficial mode of application onto titanium implant surfaces still requires investigation. Unspecific fibronectin adsorption on titanium dioxide (TiO2) surfaces can result in low-loading, high-desorption rates and protein–metal interactions with impaired biologic activity. The aim of the present study was to monitor the osteogenic cell responses (cell adhesion, proliferation, and differentiation) specifically to fibronectin biofunctionalized TiO2. Materials and Methods An innovative biomimetic streptavidin-biotin layer…
Effects of pulsed electric fields on water-soluble vitamins and ACE inhibitory peptides added to a mixed orange juice and milk beverage
2007
Abstract The effects of pulsed electric fields technology (15–40 kV/cm; 0–700 μs) and thermal processing (84 °C and 95 °C, 15–120 s) were studied on an orange juice and milk mixed beverage fortified with water-soluble vitamins (biotin, folic acid, pantothenic acid and riboflavin) and angiotensin-I-converting enzyme (ACE) inhibitory peptides. The evaluation of the technologies was carried out from two points of view: effect of treatments and effect of storage (4 °C, 81 days). The results confirmed the stability of the vitamins and the ACE inhibitory activity after the PEF treatment and during storage.
Protein S-nitrosylation: specificity and identification strategies in plants
2015
SPE Pôle IPM UB; International audience; The role of nitric oxide (NO) as a major regulator of plant physiological functions has become increasingly evident. To further improve our understanding of its role, within the last few years plant biologists have begun to embrace the exciting opportunity of investigating protein S-nitrosylation, a major reversible NO-dependent post-translational modification (PTM) targeting specific Cys residues and widely studied in animals. Thanks to the development of dedicated proteomic approaches, in particular the use of the biotin switch technique (BST) combined with mass spectrometry, hundreds of plant protein candidates for S-nitrosylation have been identi…
Identification and functional characterization of S-nitrosated proteins from Klebsormidium nitens, a model alga to study plant adaptation to land
2022
The small gaseous molecule nitric oxide (NO) is well established as a major ubiquitous component of cell signalling. A key signalling mechanism mediating NO effects is S-nitrosation, a post-translational modification by which NO can impact the target protein activities, subcellular localizations, and capacities to form protein complexes. The identification of proteins targeted by NO is of major interest in order to elucidate NO functions. Interestingly, land plants lack NO synthase (NOS), which is the main enzyme for NO synthesis in metazoans, while a few algal species possess it, thus raising many interrogations. Therefore, we focused on the identification of S-nitrosated proteins during s…
Identification and functional characterization of S-nitrosated proteins from Klebsormidium nitens in response to salt stress
2023
The small gaseous molecule nitric oxide (NO) is well established as a major ubiquitouscomponent of cell signalling. A key signalling mechanism mediating NO effects is Snitrosation, a post-translational modification by which NO can impact the target proteinactivities, subcellular localizations, and capacities to form protein complexes. The identificationof proteins targeted by NO is of major interest in order to elucidate NO functions. Interestingly,land plants lack NO synthase (NOS), which is the main enzyme for NO synthesis in metazoans,while a few algal species possess it, thus raising many interrogations. Therefore, we focused onthe identification of S-nitrosated proteins during salt str…
A simple strategy based on photobiotin irradiation for the photoelectrochemical immobilization of proteins on electrode surfaces
2006
Abstract A photoactivable organic polymer was prepared first by electrogeneration of a conductive biotinylated polypyrrole film in acetonitrile electrolyte. The successive anchoring of avidin and photobiotin led to a multilayer configuration. The latter was illuminated with light (wavelength 370–400 nm) in the presence of proteins adsorbed onto its surface. The irradiation allowed the covalent linking of the proteins to the modified electrode. As a result of the photochemical reaction, a monolayer of enzyme (glucose oxidase, GOX or alkaline phosphatase, AP) was covalently bound to the photobiotin-modified surface with retention of their catalytic activities. The surfacic activities were 34 …