Search results for "blas"
showing 10 items of 2217 documents
Cloning and characterization of CSP37, a novel gene encoding a putative membrane protein of Candida albicans.
1997
In the course of an analysis of the functions and assembly of the cell wall of Candida albicans, we have cloned and characterized a gene, which we designated CSP37 (cell surface protein), encoding a 37-kDa polypeptide which is a membrane-associated protein. The gene was isolated by immunological screening of a DNA library constructed from mycelial cells with a polyclonal serum raised against cell walls of this morphology. Analysis of the nucleotide sequence of a corresponding genomic DNA fragment revealed a single open reading frame which encodes a predicted protein of 321 amino acids with no significant homology to others in the databases. Disruption of the CSP37 gene by the method describ…
Vietējo pašvaldību problēmas zilo pludmaļu piesārņojošo atkritumu situācijas uzlabošanā
2019
Jūras piesārņojošo atkritumu (JPA) galvenais cēlonis ir cilvēka saimnieciskas vai nesaimnieciskas darbības sekas. Lai mazinātu atkritumu nonākšanu pasaules okeānā, sabiedrības ieradumus, attiecībā uz atkritumiem, ir nepieciešams mainīt uz aprites ekonomikas balstītiem pamaprincipiem. Lai piemērotu efektīvas rīcības JPA samazināšanai, būtiski ir aptvert JPA avotus, cēloņus, ietekmes un tendences nākotnē. Bakalaura darba mērķis ir apzināt pašvaldībām iespējamos JPA rašanās riskus un avotus, iegūtos rezultātus analizēt, lai piemērotu efektīvas rīcības, kas mazina draudus atkritumiem rasties un nonākt jūrās. Lai gan Zilā karoga pašvaldībām jau ir labas vides pārvaldības risinājumi, tomēr kampaņ…
Molecular docking and pharmacogenomics of vinca alkaloids and their monomeric precursors, vindoline and catharanthine.
2011
International audience; Vinblastine and vincristine are dimeric indole alkaloids derived from (formerly: ). Their monomeric precursor molecules are vindoline and catharanthine. While vinblastine and vincristine are well-known mitotic spindle poisons, not much is known about vindoline and catharanthine. Vindoline and catharanthine showed weak cytotoxicity, while vinblastine, vincristine, and the semisynthetic vindesine and vinorelbine revealed high cytotoxicity towards cancer cells. This may reflect a general biological principle of poisonous plants. Highly toxic compounds are not only active towards predators, but also towards plant tissues. Hence, plants need mechanisms to protect themselv…
Adénocarcinome de la glande lacrymale dans un rétinoblastome bilatéral traité avec radiothérapie externe : à propos d’un cas
2011
This clinical case report describes the clinical findings and diagnosis of lacrimal gland adenocarcinoma that developed 20 years after external beam radiotherapy in the treatment of bilateral retinoblastoma. Visual acuity, slit lamp biomicroscopy, fundus color photography, nuclear magnetic resonance, lateral orbitotomy and histological analysis are described.
Recepción wagneriana en Valencia: 1874-1914
2012
Esta tesis estudia la recepción wagneriana en la ciudad de Valencia en el periodo 1874-1914. No se recoge la influencia de la obra wagneriana sobre otras creaciones, sino la presencia de la obra de Wagner y las referencias explícitas a la misma. Las condiciones económicas, políticas y culturales de la ciudad así como las características multiformes que en otros lugares tiene la recepción wagneriana de la época, son expuestas de manera introductoria. La naturaleza de la recepción valenciana lleva a estructurar la información positiva en dos secciones bien distintas: los acontecimientos escénicos en la ciudad y las obras literarias que se escriben sobre Wagner o con importantes referencias a …
PETECHIAL BRAIN HAEMORRHAGES IN ACUTE LYMPHOBLASTIC LEUKAEMIA.
2013
A 37-year-old office worker was referred to our hospital with severe weakness, malaise, headache and altered mental status. He was mildly febrile (37.5°C) with reported episodes of agitation. A blood count revealed 709 720 white blood cells (WBC) / μl with 33 000 platelets/μl. The clinical, imaging and laboratory workup led to a diagnosis of acute lymphoblastic leukaemia (ALL) …
Wee1 inhibition potentiates Wip1-dependent p53-negative tumor cell death during chemotherapy
2016
AbstractInactivation of p53 found in more than half of human cancers is often associated with increased tumor resistance to anti-cancer therapy. We have previously shown that overexpression of the phosphatase Wip1 in p53-negative tumors sensitizes them to chemotherapeutic agents, while protecting normal tissues from the side effects of anti-cancer treatment. In this study, we decided to search for kinases that prevent Wip1-mediated sensitization of cancer cells, thereby interfering with efficacy of genotoxic anti-cancer drugs. To this end, we performed a flow cytometry-based screening in order to identify kinases that regulated the levels of γH2AX, which were used as readout. Another criter…
Oleuropein multicompartment nanovesicles enriched with collagen as a natural strategy for the treatment of skin wounds connected with oxidative stres…
2021
Aim: Collagen-enriched transfersomes, glycerosomes and glytransfersomes were specifically tailored for skin delivery of oleuropein. Methods: Vesicles were prepared by direct sonication and their main physicochemical and technological properties were measured. Biocompatibility, protective effect and promotion of the healing of a wounded cell monolayer were tested in vitro using fibroblasts. Results: Vesicles were mainly multicompartment, small (∼108 nm), slightly polydispersed (approximately 0.27) and negatively charged (~-49 mV). Oleuropein was incorporated in high amounts (approximately 87%) and vesicles were stable during four months of storage. In vitro studies confirmed the low toxicit…
Experimental change of cytoplasmic composition can convert determination of blastomeres inPlatynereis dumerilii (Annelida, Polychaeta)
1991
Early development ofPlatynereis dumerilii is characterized by an extremely constant cleavage pattern in which the volumes and cytoplasmic contents of the blastomeres show remarkably little variability (Dorresteijn 1990). In order to test the necessity of a precise partitioning of the cytoplasm, we have stratified the ooplasm by mild centrifugation (10 min at 300 g) after completion of meiosis but before first cleavage. The cytoplasm of the zygote stratifies randomly with respect to the pre-existing animal-vegetal axis, but first cleavage follows the animal-vegetal axis dividing the plasm before it has rearranged to its normal distribution. As usual, first cleavage is unequal in the majority…
Stilbenes and resveratrol metabolites improve mitochondrial fatty acid oxidation defects in human fibroblasts
2014
International audience; Background: Inborn enzyme defects of mitochondrial fatty acid beta-oxidation (FAO) form a large group of genetic disorders associated to variable clinical presentations ranging from life-threatening pediatric manifestations up to milder late onset phenotypes, including myopathy. Very few candidate drugs have been identified in this group of disorders. Resveratrol (RSV) is a natural polyphenol with anti-oxidant and anti-inflammatory effects, recently shown to have beneficial metabolic properties in mice models. Our study explores its possible effects on FAO and mitochondrial energy metabolism in human cells, which are still very little documented.Methods: Using cells …