Search results for "bp"

Mass spectrometry for the study of protein-ligand interactions and supramolecular complexes: Application to an intrinsically disordered protein

2015

International audience

Numerical models contribute to expand the sweet taste chemical space

2021

Le réchauffement climatique diminue-t-il le risque de dégâts par le gel pour les cultures de climat tempéré ?

2014

Does global warming reduce the freezing injury risk to temperate climate crops? Winter crop response to a temperature increase is complex. Recent works point out, for cold climate vegetation, a paradoxical increase in freezing injury in a warming climate. Complementary works are needed to support these results for winter crops in temperate areas. To achieve this goal, five climatic records spanning a fifty years-long period for both daily minimum and maximum temperatures were analyzed with a model predicting frost hardiness and freezing damage for the winter pea crop. The methodological approach was based on three main steps: 1) the climate warming analysis of the 1987/1988 temperature shif…

Dual role of acyl-coa binding protein in the hypothalamus: regulator of astrocyte fatty acid metabolism and gliotransmitter targeting pomc neurons

2013

La liste des auteurs est incomplète.; absent

Decoding sweet taste from chemical structures

2019

International audience

New technologies getting informations on repartition, orientation and identification of biomolecules on SPR chip

2008

We present herein an original approach, consisting to lead in parallel a global analysis (SPR) and nanoscale characterizations (AFM, mass spectrometry, TOF-SIMS) of bio-molecules on the biochip surface. The questions we want to answer concern especially the organization, orientation and identification of immobilized and captured biomolecules. In order to characterize the sensing layer in terms of molecular structure and orientation, we engaged a SPR-TOF SIMS first set of experiments (collaboration: Pr Aoyagi, Japan). Our results demonstrated that analysis of orientation of the immobilized cytochrome b5 is possible (ref2&3) and help to optimize and develop our sensing layer. We also recently…

Technological innovation around protein and cell biochip for diagnosis: a translational research from nanoworld to patient

2009

International audience; A great challenge in biosensors and diagnosis devices relies on the way to reconstitute relevant biological mechanisms on surface of the biochips and which analytical tools are convenient to provide accurate and rapid information on the structures and function of molecules attached to this surface. A better control in the realization of biochips can be obtained in combining different complementary approaches while always keeping in mind the biological key point. Researches in CLIPP are focused towards this objective. Conception, realization and characterization of protein and cell chips are presented. We detail different strategies of materials engineering1,2,3, chem…

Cell imaging connected with protein mapping to investigate glyphosate induced toxicity on human keratinocytes

2009

During the skin ageing which is accelerated by environmental factors, the generated reactive oxygen species make unstable the "proliferation/differentiation/apoptosis" coupling of epidermal cells. A biochemical study, previously realized on human keratinocyte cultures treated by a pesticide, glyphosate, allowed the validation of an in vitro model of skin ageing. In this work, we propose to study the loss of HaCaT cell integrity appearing after this chemical induced-oxidative stress. An original approach, combining a micro- to nanoscale cell characterization and analysis of fluctuating protein expression, is proposed: a) the exploration of cell growth and morphology through fluorescence and …

Concept of dynamic DNA network dedicated to DNA-Protein interactions studies

2007

The approach lies on the development of an original DNA biosensor. This self-assembled DNA biochip is constituted of a tethered lipid membrane presenting « protein/DNA » complexes. This unique assembly, using a highly flexible bifunctional molecular spacer and a fluidic matrix, allows the control of oligonucleotides surface density and their mobility thus favouring hybridization of the DNA probes in liquid environment and under weak constraints. Recent Surface Plasmon Resonance experiments lead to building of protein/DNA dimers presenting two special DNA sequences recognized by regulation proteins. We demonstrated the capacity and relevance of this biosensor in the case of an estrogen recep…

Changes in properties of palm sugar syrup produced by an open pan and a vacuum evaporator during storage

2013

International audience; The aim of this study was to monitor the changes in the properties of palm sugar syrup produced by an open pan and a vacuum evaporator at 70°C and 80°C during storage under 4°C and room temperature (30°C) for 12 months at monthly intervals. During storage, Maillard reaction took place in samples stored under 4°C lower than those stored under 30°C. This was shown by lower a* values, intermediate browning products (IBP), browning intensity (BI) and HMF content, and higher L* values, fructose, glucose and free amino group contents during storage for 12 months. HMF, a possible mutagen formed by nonenzymatic browning during the heating and storage of sugar based products,…