Search results for "comet"

Genotoxicity and Epigenotoxicity of Carbazole-Derived Molecules on MCF-7 Breast Cancer Cells

2021

The carbazole compounds PK9320 (1-(9-ethyl-7-(furan-2-yl)-9H-carbazol-3-yl)-N-methylmethanamine) and PK9323 (1-(9-ethyl-7-(thiazol-4-yl)-9H-carbazol-3-yl)-N-methylmethanamine), second-generation analogues of PK083 (1-(9-ethyl-9H-carbazol-3-yl)-N-methylmethanamine), restore p53 signaling in Y220C p53-mutated cancer cells by binding to a mutation-induced surface crevice and acting as molecular chaperones. In the present paper, these three molecules have been tested for mutant p53-independent genotoxic and epigenomic effects on wild-type p53 MCF-7 breast adenocarcinoma cells, employing a combination of Western blot for phospho-γH2AX histone, Comet assay and methylation-sensitive arbitrarily pr…

Molecular Signatures Associated with Treatment of Triple-Negative MDA-MB231 Breast Cancer Cells with Histone Deacetylase Inhibitors JAHA and SAHA

2017

Jay Amin Hydroxamic Acid (JAHA; N8-ferrocenylN1-hydroxy-octanediamide) is a ferrocene-containing analogue of the histone deacetylase inhibitor (HDACi) suberoylanilide hydroxamic acid (SAHA). JAHA’s cytotoxic activity on MDA-MB231 triple negative breast cancer (TNBC) cells at 72 h has been previously demonstrated with an IC50 of 8.45 M. JAHA’s lethal effect was found linked to perturbations of cell cycle, mitochondrial activity, signal transduction and autophagy mechanisms. In order to glean novel insights on how MDA-MB231 breast cancer cells respond to the cytotoxic effect induced by JAHA, and to compare the biological effect with the related compound SAHA, we have employed a combination of…

2015

Head and neck squamous cell cancer (HNSCC) is the 7th most common cancer worldwide. Despite the development of new therapeutic agents such as monoclonal antibodies, prognosis did not change for the last decades. Cold atmospheric plasma (CAP) presents the most promising new technology in cancer treatment. In this study the efficacy of a surface micro discharging (SMD) plasma device against two head and neck cancer cell lines was proved. Effects on the cell viability, DNA fragmentation and apoptosis induction were evaluated with the MTT assay, alkaline microgel electrophoresis (comet assay) and Annexin-V/PI staining. MTT assay revealed that the CAP treatment markedly decreases the cell viabil…

A computational approach for the assessment of executive functions in patients with obsessive-compulsive disorder

2019

Previous studies on obsessive–compulsive disorder (OCD) showed impairments in executive domains, particularly in cognitive inhibition. In this perspective, the use of virtual reality showed huge potential in the assessment of executive functions; however, unfortunately, to date, no study on the assessment of these patients took advantage of the use of virtual environments. One of the main problems faced within assessment protocols is the use of a limited number of variables and tools when tailoring a personalized program. The main aim of this study was to provide a heuristic decision tree for the future development of tailored assessment protocols. To this purpose, we conducted a study that…

Attachment Styles and Suicide-Related Behaviors in Adolescence: The Mediating Role of Self-Criticism and Dependency

2017

Insecure attachment and the personality dimensions of self-criticism and dependency have been proposed as risk factors for suicide in adolescents. The present study examines whether self-criticism and dependency mediate the relationship between insecure attachment styles and suicidality. A sample of 340 high-school students (73.2% females), ranging in age from 13 to 20 years (M = 16.47, SD = 1.52), completed the Depressive Experiences Questionnaire for Adolescents, the Depressive Experiences Questionnaire for Adolescents, the Attachment Style Questionnaire, and the Suicidal Behaviors Questionnaire-Revised. The results partially support the expected mediation effects. Self-criticism, but not…

Kinetics of Cometabolic Biodegradation of 4-Chlorophenol and Phenol by Stenotrophomonas Maltophilia KB2

2018

The cometabolic biodegradation of 4-Chlorophenol (4-CP) by the Stenotrophomonas maltophilia KB2 strain in the presence of phenol (P) was studied. In order to determine the kinetics of biodegradation of both substrates, present alone and in cometabolic systems, a series of tests was carried out in a batch reactor changing, in a wide range, the initial concentration of both substrates. The growth of the tested strain on phenol alone was described by Haldane kinetic model (mm = 0:9 1/h, Ksg = 48:97 gg/m3, KIg = 256:12 gg/m3, Yxg = 0:5715). The rate of 4-CP transformation by resting cells of KB2 strain was also described by Haldane equation and the estimated parameters of the model were: kc = 0…

Kinetics of cometabolic biodegradation of 4-chlorophenol and phenol by Stenotrophomonas maltophilia KB2

2018

The cometabolic biodegradation of 4-Chlorophenol (4-CP) by the Stenotrophomonas maltophilia KB2 strain in the presence of phenol (P) was studied. In order to determine the kinetics of biodegradation of both substrates, present alone and in cometabolic systems, a series of tests was carried out in a batch reactor changing, in a wide range, the initial concentration of both substrates. The growth of the tested strain on phenol alone was described by Haldane kinetic model (mm=0:9 1/h,Ksg=48:97 gg/m3,KIg=256:12 gg/m3, Yxg=0:5715). The rate of 4-CP transformation by resting cells of KB2 strain was also described by Haldane equation and the estimated parameters of the model were:kc=0:229 gcc =gxh…

The Philae lander mission and science overview.

2017

The Philae lander accomplished the first soft landing and the first scientific experiments of a human-made spacecraft on the surface of a comet. Planned, expected and unexpected activities and events happened during the descent, the touch-downs, the hopping across and the stay and operations on the surface. The key results were obtained during 12–14 November 2014, at 3 AU from the Sun, during the 63 h long period of the descent and of the first science sequence on the surface. Thereafter, Philae went into hibernation, waking up again in late April 2015 with subsequent communication periods with Earth (via the orbiter), too short to enable new scientific activities. The science return of the…

Sublimation of icy aggregates in the coma of comet 67P/Churyumov–Gerasimenko detected with the OSIRIS cameras on board Rosetta

2016

Beginning in 2014 March, the OSIRIS (Optical, Spectroscopic, and Infrared Remote Imaging System) cameras began capturing images of the nucleus and coma (gas and dust) of comet 67P/Churyumov¿Gerasimenko using both the wide angle camera (WAC) and the narrow angle camera (NAC). The many observations taken since July of 2014 have been used to study the morphology, location, and temporal variation of the comet's dust jets. We analysed the dust monitoring observations shortly after the southern vernal equinox on 2015 May 30 and 31 with the WAC at the heliocentric distance Rh = 1.53 AU, where it is possible to observe that the jet rotates with the nucleus. We found that the decline of brightness a…

Avaluació psicomètrica de l'estimació en numeració i càlcul a l'educació primària

2006

L'estimació en numeració i càlcul ha pres rellevància en les dues darreres dècades per la seva importància i necessitat social i, per aquest motiu, ha estat progressivament inclosa en els currículums de matemàtiques de l'educació obligatòria. Tanmateix, el treball de l'estimació a l'aula es veu reduït freqüentment a les tècniques d'arrodoniment i els alumnes no es mostren hàbils a l'hora de desenvolupar estratègies d'estimació o de decidir quan un càlcul estimat és adient per a una situació problemàtica. En aquest article es presenta un test adreçat a estudiar els processos utilitzats en tasques d'estimació en numeració i càlcul per infants que han acabat l'educació primària. S'explica el m…