Search results for "complement"

La diversité de consommation de fromages dans la petite enfance : un facteur protecteur des maladies allergiques ?

2015

Introduction Une diversité alimentaire importante et précoce a été montrée protectrice vis à vis des maladies allergiques (Roduit C. et al, JACI 2012 Jul;130(1):130-6). L'effet de la diversité de consommation de fromages, riches en microorganismes, n'a pas été étudié. Or l'exposition aux microorganismes est suspectée pour son rôle protecteur sur la survenue de maladies allergiques. Le but de notre étude était d'évaluer l'effet protecteur de la diversité de consommation de fromages vis-à-vis de maladies allergiques (MA) : allergie alimentaire (AA), dermatite atopique (DA), asthme (AS), rhinite allergique (RA) et bronchite (BR). Méthodes L'étude a porté sur 941 enfants de la cohorte PATURE (P…

Complement Receptor Analogous Factors in Human Serum: I. Isolation of a Molecule Inhibitory for Complement Dependent Rosette Formation, its Identific…

1979

Abstract A glycoprotein was isolated from human plasma which partially inhibited C3 carrying erythrocytes from binding to complement receptor cells (CR + C). Based on its physicochemical characteristics and its antigenicity this glycoprotein was identified as aI-antitrypsin (α 1 -AT). The activity of α 1 -AT towards-C3 and its fragments was unaffected by heating but it was destroyed by periodic acid. The isolated carbohydrate moiety of α 1 -AT showed the same effect as the intact molecule. Using F(ab) 2 of IgG-anti-α 1 -AT, α 1 -AT could be demonstrated on Raji cells and human erythrocytes. Treatment of these CR + C with IgG-anti-α 1 -AT resulted in a blockade of their C3 receptor activity.…

Chloroplastic glutamine synthetase from Brassica napus.

1993

The second component of human complement: Detection of two hemolytic forms in plasma by pH Variation

1988

The second component of human complement (C2) in pseudoglobulin prepared from normal plasma eluted as a single peak at high conductivity (30 mS) and pH 4.5 from the cationic exchangers S-Sepharose or Mono S in the Fast Protein Liquid Chromatography (FPLC) System. The C2 was stable at pH 4.5 and 0 degrees C if enzyme inhibitors were used and the pH was raised to 6.0 after elution from the columns. After rechromatography on Mono S in the FPLC System at the median isoelectric point of 5.5 or pH 6.0, the C2 eluted as two distinct hemolytic forms: the first peaked at 16 mS, the second at 30 mS. The two forms of C2 did not correlate with the allotypic variant of C2 in individual, normal human pla…

Characterisation of Diarylheptanoid- and Flavonoid-type Phenolics in Corylus avellana L. Leaves and Bark by HPLC/DAD-ESI/MS

2013

Introduction The leaves of Corylus avellana L. (common hazel, Betulaceae), a plant with a wide distribution in Europe, have been used in folk medicine for various diseases, but phytochemical exploration of C. avellana is still incomplete. To the best of our knowledge there is no previous report concerning diarylheptanoids in C. avellana, although these compounds show a frequent occurrence among Betulaceae plants. Objective To improve existing online chromatographic methods for the investigation of the phenolic compounds in C. avellana leaves and bark, focusing on diarylheptanoid-type molecules. Methods Dried and powdered leaves and bark of C. avellana were extracted with increasing polarity…

Putative multiadhesive protein from the marine spongeGeodia cydonium: Cloning of the cDNA encoding a fibronectin-, an SRCR-, and a complement control…

1998

Sponges (Porifera) representing the simplest metazoan phylum so far have been thought to possess no basal lamina tissue structures. One major extracellular matrix protein that is also a constitutive glycoprotein of the basal lamina is fibronectin. It was the aim of the present study to identify the native protein from the marine sponge Geodia cydonium and to isolate the corresponding cDNA. In crude extracts from this sponge protein(s) of Mr of Ý230 and Ý210 kDa could be visualized by Western blotting using an anti-fibronectin [human] antibody. By PCR cloning from a cDNA library of G. cydonium we isolated a cDNA comprising one element of fibronectin, the type-III (FN3) module. The cDNA (2.3 …

Description of a Simple, Specific, and Sensitive Test for the Detection of Detergent-Solubilized C3b Receptor

1981

Abstract The C3b receptor was isolated from detergent-solubilized human erythrocyte membranes by a previously described technique (1). The receptor glycoprotein was shown to enhance EAC14oxy23b rosette formation with Raji lymphoblastoid cells. This provided a specific and sensitive test to detect the solubilized C3b receptor either in crude or highly purified form. The property of the C3b receptor tested by this assay appears to be analogous to properties of β1H.

Glutamine synthetase from roots of Brassica napus. Nucleotide sequence of a cytosolic isoform.

1994

Expression and Expressional Control of Nitric Oxide Synthases in Various Cell Types

1995

Publisher Summary Nitric oxide (NO) can produce posttranslational modifications of proteins (via ADP ribosylation) and is capable of destroying parasites and tumor cells by inhibiting iron-containing enzymes or directly interacting with the DNA of these cells. In view of this multitude of functions of NO, it is important to understand how cells accomplish and regulate their NO production. Three isozymes of NOS have been identified, and their protein, cDNA, and genomic DNA structures have been elucidated. In humans NOS I, II, and III are encoded by three different genes, located on chromosomes 12, 17, and 7 respectively. The cDNAs for these enzymes have been isolated. All NOS isozymes oxidiz…

The Role of Lectins in Finfish: A Review

2019

The immune system of vertebrates involves both innate and acquired immune responses. The innate immunity is more generalized with robust response whereas the other has a highly specific response to infectious pathogens. Because of the lack of specialized lymphatic organs, innate immunity is an important mode of defense in fishes. The less specific innate immune system acts mainly through complement pathway which depends on pattern-based recognition of “self” and “non-self” targets by host lectins and associated proteins. This ultimately results in the clearance of target cells. Lectins are glycoproteins which possess at least one carbohydrate recognition domain (CRD) that specifically and r…