Search results for "corpus"

The Candida albicans UBI3 gene encoding a hybrid ubiquitin fusion protein involved in ribosome biogenesis is essential for growth.

2003

We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes a ubiquitin fusion protein involved in ribosome biogenesis. A one-step gene disruption procedure, using the plasmid pCaDis, was designed to place the second copy of the UBI3 gene under the control of the tightly regulated MET3 promoter in a C. albicans heterozygous strain (UBI3/Deltaubi3::hisG), previously isolated in the first step of the ura-blaster protocol. Analysis of the conditional null mutant in repressing and inducing conditions indicates that UBI3 is an essential gene whose expression is required for growth of C. albicans.

IDENTIFICATION OF A NOVEL COMPOUND HETEROZYGOUS MUTATION OF SPG11 IN A PATIENT AFFECTED BY HSPWITH THIN CORPUS CALLOSUM

2010

Role of Pir1 in the construction of the Candida albicans cell wall

2004

Searches in a Candida albicans database (http://genolist.pasteur.fr/CandidaDB/) identified two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences showed 42 % and 45 % homology with Saccharomyces cerevisiae Pir4. The two DNA sequences are alleles of the same gene (CaPIR1) but IPF 19968 has a deletion of 117 bases. IPF 19968 encodes a putative polypeptide of 364 aa, which is highly O-glycosylated and has an N-mannosylated chain, four cysteine residues and seven repeats. Both alleles are expressed under different growth conditions and during wall construction by regenerating protoplasts. The heterozygous mutant cells are elongated, form clumps of several cells an…

Depletion of polyubiquitin encoded by the UBI4 gene confers pleiotropic phenotype to Candida albicans cells.

2003

We have studied the roles of polyubiquitin in Candida albicans physiology. Heterologous expression of the C. albicans polyubiquitin (UBI4) gene in a ubi4 Saccharomyces cerevisiae strain suppressed the mutant phenotype (hypersensitivity to heat shock). A heterozygous strain UBI4/Deltaubi4::hisG, obtained following the ura-blaster procedure, was used to construct a conditional mutant using a pCaDis derivative plasmid. By serendipity we isolated the UBI4 conditional mutant as well as a UBI4 mutant containing a non-functional MET3 promoter. Depletion of polyubiquitin conferred pleiotropic effects to mutant cells: (i) a limited increased sensitivity to mild heat shock; (ii) increased formation o…

In vitro assessment of the antifungal effects of neem powder added to polymethyl methacrylate denture base material

2018

Background Denture with antimicrobial activities is desirable to prevent Candida albican adhesion subsequently decreasing the susceptibility of denture stomatitis incidence. Azadirachta Indica, commonly known as Neem powder has antimicrobial effect but the effect of its addition to acrylic denture base on C. albicans adhesion has not been investigated. The aim of this study was determine whether adding neem powder to acrylic denture base materials could reduce Candida albicansadhesion. Material and methods One hundred and twenty acrylic resin denture specimens were fabricated and divided into heat-polymerized (n=60) and auto-polymerized (n=60) groups. Each group was further divided into 6 g…

San Juan de Ribera "de puertas adentro". Interiores domésticos-Interiores litúrgicos. Una aproximación hacia su amueblamiento y ornato (1562-1615)

2017

El presente trabajo ha tratado de recomponer las imágenes que nos trasladan los interiores litúrgicos y domésticos a los que estuvo vinculado San Juan de Ribera, desde su primera residencia y casa propia cuando fue nombrado obispo de Badajoz (1562), hasta sus residencias en territorio valenciano: el Palacio Arzobispal, la casa de la Huerta, extramuros de Valencia, el Castillo de Burjasot y el Real Colegio Seminario de Corpus Christi, conocido como el Colegio del Patriarca. Para llegar a hacer posible la recreación de esos espacios, se toma como hilo conductor las piezas de mobiliario que se conservan todavía en el Colegio de Patriarca y que en su día estudiamos cuando realizamos varias inte…

Naming MINERALITY of French white wines : a contrastive study on the emergence of a " new " wine descriptor

2016

International audience; [Context] MINERAL / MINERALITY has emerged in wine prescriptive and descriptive discourses for about 20 years, especially to characterize special kinds of French white wines like Chablis in Northern Burgundy. Despite its very popular use by both experts and consumers, it still lacks a terminological definition that would be accepted by all wine professionals. For this reason, a research program was conducted in the last two years by an interdisciplinary team at the University of Burgundy and the Wine School of Changins (Switzerland) in order to cross semantic and sensory data enabling the establishment of a prototypical definition.[Aims] Building on the results of th…

Restituye Lucrecio, el sermon, y refierele brevemente Cornelio, el resto de las Fiestas

L'Oracion panegyrica amb portadella pròpia Oracion panegyrico-festiva, que en la solemnidad de la dedicación de la nueva Iglesia del Convento Hospital de Corpus Christi de religiosos de San Juan de Dios, de la ciudad de Orihuela / decia el Doctor Don Luys Santa-Cruz y Perez ..., p. [2], 22 p. Coloquio II : Restituye Lucrecio, el sermon, y refierele brevemente Cornelio, el resto de las Fiestas, p. 23-27 Caplletres ornades, frisos, bandes Port. i portadella amb orla tip Sign.: [ ]4, *2, A-G4? Text a 1 i a 2 col. - Postil·les. - Reclams

Candida biotypes in patients with oral leukoplakia and lichen planus. Candida biotypes in leukoplakia and lichen planus.

1996

Prevalence of yeasts in 35 leukoplakia and 34 oral lichen planus patients was compared with that observed in persons without oral diseases. Serotype and morphotype were determined on Candida albicans isolates. Yeasts were isolated from the oral cavity specimens of 43.7% of the patients. C. albicans (serotype A) was the predominant species (76% in leukoplakia, 88.2% in lichen planus and 60.8% in healthy persons). Sixteen morphotypes were encountered on malt extract agar, being 732, 733, 734, 753 and 754 the most frequently found. Morphotypes SP1N and SP1Y were the most common on Sabouraud-trypheniltetrazolium agar (68.4% of the isolates from leukoplakia and 73.3% from lichen planus, but only…

Antigenic cell wall mannoproteins in Candida albicans isolates and in other Candida species.

1991

Polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs), raised against mannoprotein components from Candida albicans ATCC 26555 (serotype A) blastoconidia and mycelial cell walls, were used to investigate antigenic similarities among wall mannoproteins from other C. albicans serotype A and B strains, and from C. tropicalis and C. guilliermondii. Radioactively labelled walls isolated from cells grown at either 28 degrees C or 37 degrees C were digested with a beta-glucanase complex (Zymolyase 20T) to release cell-wall-bound mannoproteins. Numerous molecular species with different electrophoretic mobilities were released from the various isolates. Differences appeared to be related to …