Search results for "corpus"
showing 10 items of 698 documents
The Corpus of Advanced Learner Finnish (LAS2): Database and toolkit to study academic learner Finnish
2014
This paper introduces the Corpus of Advanced Learner Finnish (LAS2), one of the existing corpora of learner Finnish. The corpus was started at the University of Turku in 2007, and the initial motivation for its collection was to make it possible to deal with novel linguistic challenges posed by academic immigration and to contribute to corpus linguistics, Finnish linguistics and the study of second language acquisition. This paper describes the typological standpoint of the LAS2, its position with respect to other corpora of learner Finnish, the compilation criteria, the annotation applied and the workflow implemented. The corpus consists of three subcorpora of written academic texts of non…
La atenuación en aprendices francófonos de E/LE como manifestación de la competencia pragmática
2018
Esta investigación se centra en el estudio del fenómeno pragmático de la atenuación, fenómeno escasamente trabajado en la enseñanza/aprendizaje de E/LE, a pesar de ser fundamental en el desarrollo de la competencia comunicativa de los aprendices de lenguas. Se trata de un estudio empírico, realizado sobre el análisis de los datos obtenidos en un corpus de lengua oral de aprendices, de creación propia. El objetivo fundamental de esta investigación ha sido doble: estudiar la competencia pragmática atenuadora en la interlengua de aprendices de español y elaborar una propuesta de su mejora y desarrollo a partir de la reflexión metapragmática de sus propias producciones lingüísticas. Estos fines…
The genotypes and virulence attributes of C. albicans isolates from oral leukoplakia
2021
Background There is a debate as to whether some types of oral leucoplakias (OL) are caused by Candida species, and whether they contribute to the malignant transformation, associated with a minority of such lesions. As no detailed population analysis of yeast isolates from OL is available, we evaluated the virulence attributes, and genotypes of 35 C. albicans from OL, and compared their genotypes with 18 oral isolates from healthy individuals. Material and Methods The virulence traits evaluated were esterase, phospholipase, proteinase, haemolysin and coagulase production, and phenotypic switching activity, and yeast adherence and biofilm formation. DNA from OL and control yeasts were evalua…
Die Auswirkung sprachbiographischer und sprachideologischer Faktoren auf den Sprachgebrauch bilingualer Sprecher:innen
2023
Współcześnie w badaniach nad wielojęzycznością panuje zgoda co do tego, że mówcy bilingwalni nie są podwójnie jednojęzyczni, ale języki, którymi się posługują, wzajemnie na siebie wpływają. Zakres i forma tego oddziaływania są uwarunkowane różnymi czynnikami, m.in. biografiami i ideologiami językowymi. Artykuł odwołuje się do wyników badań prowadzonych w ramach polsko-niemieckiego projektu Pokoleniowe zróżnicowanie języka: zmiany morfosyntaktyczne wywołane przez polsko-niemiecki kontakt językowy w mowie osób dwujęzycznych, w którym przebadano dwie różniące się od siebie generacje – osoby z wczesną dwujęzycznością w Polsce (Generacja Polska/GP) oraz osoby z późną dwujęzycznością w Niemczech …
Anchorage of Candida albicans Ssr1 to the cell wall, and transcript profiling of the null mutant.
2005
Incorporation into the wall of Candida albicans Ssr1, a GPI-dependent protein, was investigated by construction of different truncated genes for which the three potential omega sites (S199, S215 and G216) and the corresponding omega+1 and omega+2 were eliminated or modified. Cells of the C. albicans ssr1Delta mutant were transformed with pADH-pl harboring the truncated versions of CaSSR1, pADH-DeltaCaSSR1t(217-234) (lacking a C-terminal hydrophobic stretch of 18 aa including the putative omega+2 and omega+1, omega+2 of S215 and G216) or pADH-DeltaCaSSR1t(199-201) (lacking three serine residues), and their walls were analyzed for the protein. Results suggested that the three serine residues …
Identification of glucan-mannoprotein complexes in the cell wall of Candida albicans using a monoclonal antibody that reacts with a (1,6)- -glucan ep…
1995
The use of a novel monoclonal antibody (mAb) that reacts with (1,6)-beta-glucan has permitted the study of the different covalent linkages between glucan and mannoproteins in the cell wall of Candida albicans. The mAb JRR1 was originally raised by immunization with Zymolyase extracts from C. albicans cell walls, but it soon became apparent that it reacted with a (1,6)-beta-glucan epitope. By using this antibody, we show the existence of glucan-mannoprotein complexes between the (1,6)-beta-glucan epitope recognized by the antibody and cell wall mannoproteins. The topology of the (1,6)-beta-glucan in the cell wall of C. albicans has also been studied.
Identification ofCandida albicansclinical isolates by PCR amplification of anEFB1gene fragment containing an intron-interrupted open reading frame
2000
The use of a single pair of primers, deduced from the intron and exon nucleotide sequences of the Candida albicans EFB1 gene, in polymerase chain reaction (PCR) assays performed with whole cells of both laboratory strains and clinical isolates of Candida species, resulted in the species-specific amplification of a 785 bp DNA fragment in C. albicans strains. Clinical C. albicans isolates were tested, and 85 out of 86 generated the expected PCR-amplified product; other Candida species, both laboratory strains and clinical isolates, as well as laboratory strains belonging to other fungal genera, including medically relevant taxa, failed to amplify any DNA fragment. In addition, unusual C. albi…
A method for taxonomic determination ofCandida albicans with DNA probes
1993
Determination of Candida species represents an important problem derived from the clinical implications of the species belonging to this genus. DNA probes have already been used for the epidemiology of Candida albicans, as well as for taxonomic analysis of Candida and other genera, although these probes are based on non-species-specific DNA sequences. In this work we carried out a 48-h assay, allowing the identification of C. albicans from clinical isolates, using DNA probes based on C. albicans LEU2 and URA3 genes. Another probe related to C. albicans SEC18 gene was shown not to be C. albicans specific.
Candida-associated denture stomatitis
2010
Candida albicans is a dimorphic yeast strongly gram positive able to live as normal commensal organism in the oral cavity of healthy people. It is the yeast more frequently isolated in the oral cavity. Under local and systemic factors related to the host conditions, it becomes virulent and responsible of oral diseases known as oral candidiasis. It has been shown that the presence of denture is a predisposing factor to the onset of pathologies related to C. albicans. Clinical studies have shown that C. albicans is not only able to adhere to the mucous surfaces, but also to stick to the acrylic resins of the dental prostheses. Both the plaque accumulated on the denture and the poor oral hygie…
The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshi…
2002
We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of exponentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. …