Search results for "cycle"

The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors

2010

Abstract ISWI is an evolutionarily conserved ATP-dependent chromatin remodeling factor playing central roles in DNA replication, RNA transcription, and chromosome organization. The variety of biological functions dependent on ISWI suggests that its activity could be highly regulated. Our group has previously isolated and characterized new cellular activities that positively regulate ISWI in Drosophila melanogaster. To identify factors that antagonize ISWI activity we developed a novel in vivo eye-based assay to screen for genetic suppressors of ISWI. Our screen revealed that ISWI interacts with an evolutionarily conserved network of cellular and nuclear factors that escaped previous genetic…

DNA adduct levels associated with p53 induction and delay of MCF-7 cells in S phase after exposure to benzo[g]chrysene dihydrodiol epoxide enantiomer…

1998

Optically active isomers of a mammary carcinogen, anti-benzo[g]chrysene 11, 12-dihydrodiol 13, 14-epoxide, react to different extents with DNA and generate DNA adducts that differ in their stereochemistry. In the study reported here, the effect of these two enantiomers on the progress of human breast carcinoma MCF-7 cells through the cell cycle was investigated. Each enantiomer caused the cells to accumulate in the S phase, but a higher dose of the benzo[g]chrysene 11S, 12R-dihydrodiol 13R, 14S-epoxide than of its enantiomer was required to induce this effect. Similarly, induction of p53 also required a higher dose of benzo[g]chrysene 11S, 12R-dihydrodiol 13R, 14S-epoxide. Postlabeling stud…

New benzothieno[3,2-d]-1,2,3-triazines with antiproliferative activity: synthesis, spectroscopic studies, and biological activity.

2014

New benzothieno[3,2-d]-1,2,3-triazines, together with precursors triazenylbenzo[b]thiophenes, were designed, synthesized and screened as anticancer agents. The structural features of these compounds prompted us to investigate their DNA binding capability through UV–vis absorption titrations, circular dichroism, and viscometry, pointing out the occurrence of groove-binding. The derivative 3-(4-methoxy-phenyl)benzothieno[3,2-d]-1,2,3-triazin-4(3H)-one showed the highest antiproliferative effect against HeLa cells and was also tested in cell cycle perturbation experiments. The obtained results assessed for the first time the anticancer activity of benzothieno[3,2-d]-1,2,3-triazine nucleus, and…

Lack of Association Between Tumor Oxygenation and Cell Cycle Distribution or Proliferation Kinetics in Experimental Sarcomas

2003

In tumor cells, pronounced hypoxia induces an arrest of cell cycle in the late G1phase1−3. Since hypoxia is a common phenomenon in experimental and human tumors the hypoxia-induced disturbance of the cell cycle may play a role in the reduced efficacy of non-surgical treatment modalities resulting in a reduced long-term prognosis and a higher rate of local recurrences in hypoxic tumors4,5. It has been shown that a cell cycle arrest reduces the efficacy of standard radiotherapy6,7 and may alter the cytotoxic effects of various chemotherapeutic agents such as cisplatin, alkylating agents, doxorubicin or taxols8−12 and of cytokines13. If tumor hypoxia plays a relevant role in affecting the cell…

Cytotoxicities of Polysubstituted Chlorodicarbonyl(cyclopentadienyl) and (Indenyl)ruthenium Complexes

2013

Polysubstituted cyclopentadienyl and indenyl complexes of ruthenium were synthesized and investigated to elucidate their potential cytotoxic activities. In particular, substituted (indenyl)ruthenium complexes inhibited the proliferation of a panel of human adherent cancer cells with comparable activity to reference agent cisplatin. One of the active compounds exerted a concentration dependent inhibition of cell cycle at G1–S transiton as evidenced by flow cytometry.

Specific interaction of citrate with bis(fluorophoric) bibrachial lariat aza-crown in comparison with the other components of the Krebs cycle

2006

Clares Garcia, M. Paz, M.Paz.Clares@uv.es ; Garcia-España Monsonis, Enrique, Enrique.Garcia-Es@uv.es ; Soriano Soto, Concepcion, Concepcion.Soriano@uv.es ; Tejero Toquero, Roberto, Roberto.Tejero@uv.es

Metabolomics of the effect of AMPK activation by AICAR on human umbilical vein endothelial cells

2011

AMP-activated protein kinase (AMPK) is a metabolic master switch expressed in a great number of cells and tissues. AMPK is thought to modulate the cellular response to different stresses that increase cellular AMP concentration. The adenosine analog, 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR) is an AMPK activator used in many studies to assess the effects of AMPK activation on cellular metabolism and function. However, the effect of AICAR on cell metabolism reaches many different pathways and metabolites, some of which do not seem to be fully related to AMPK activation. We have now for the first time used NMR metabolomics on human umbilical vein endothelial cells (HUVEC) fo…

Citric Acid Cycle

2011

Aerobic Metabolism: Benefits from an Oxygenated World

2010

In the preceding chapter, we have emphasized the dangers that the advent of dioxygen presented to the existing anaerobic organisms, and the ways they evolved to deal with the problems. However, this is only part of the story and were it to have ended here, we and the world we know would not exist. What happened instead was quite remarkable; for life seized upon an opportunity presented by the presence of free dioxygen to become many-fold more efficient in extracting energy from foodstuffs. As we shall see, this aerobic, oxidative metabolism opened in turn a multitude of new opportunities for growth and diversification.

Changes in Cerebral Amino Acid Transport During Development

1976

The transport of metabolites to and from the central nervous system is of considerable interest. To a greater extent than most other tissues, central nervous system tissue invitro takes up amino acids to well above their concentrations in the incubation medium. Presumably the transport systems responsible for this uptake and for efflux invitro are also those responsible for transport between brain cells in living animals2.