Search results for "elements"
showing 10 items of 799 documents
The distribution of Rare Earth Elements discriminates the growth substrate of Vitis vinifera L.
2021
Sustainable agricultural, food-related strategies and geographic traceability require understanding of the plant physiological response to stress potentially generated by contaminated soils. Here, we have investigated the effect of contaminated substrate on growth of Vitis vinifera L. plants analysing the distribution of full Rare Earth Elements (REE) spectra in different parts of the plant. Experiments were carried out using pristine plants growing in a handmade substrate (blank experiment) and in REE artificially-enriched soil (spiked experiment). Our results show that both plant mass and REE amount in leaves are not influenced by the substrate enrichment while roots are by one-order of m…
Retrotranspozonu struktūra parastās priedes (Pinus sylvestris L.) genomā un to ekspresija
2014
ANOTĀCIJA Promocijas darbs izstrādāts Latvijas Valsts mežzinātnes institūtā „Silava”. Pētījuma mērķis bija identificēt aktīvos retrotranspozonus parastās priedes (Pinus sylvestris L.) genomā un raksturot to transkripcijas līmeni dažādu stresa veidu ietekmē kontrolētos apstākļos. Pētījumā izmantoti nespecifiskie retrotranspozonu marķieri balstīti uz augsti konservatīvām tRNS piesaistes vietām. Viena klona priežu rametus pakļāva dabā izplatītam karstuma stresam, kukaiņu invāzijai (priežu hermess (Pineus pini L.), kā arī mākslīgi izraisītai augu hormonu (abscīzskābes un salicilskābes) ietekmei. Darba rezultātā identificēti pārsvarā I klases mobīlo elementu pārstāvji, kā arī tos saturošie himēr…
Enhancerless Cytomegalovirus Is Capable of Establishing a Low-Level Maintenance Infection in Severely Immunodeficient Host Tissues but Fails in Expon…
2010
ABSTRACT Major immediate-early transcriptional enhancers are genetic control elements that act, through docking with host transcription factors, as a decisive regulatory unit for efficient initiation of the productive virus cycle. Animal models are required for studying the function of enhancers paradigmatically in host organs. Here, we have sought to quantitatively assess the establishment, maintenance, and level of in vivo growth of enhancerless mutants of murine cytomegalovirus in comparison with those of an enhancer-bearing counterpart in models of the immunocompromised or immunologically immature host. Evidence is presented showing that enhancerless viruses are capable of forming restr…
Elemental composition of seasoning products
2007
The elemental composition of seasoning products, like spices garlic and onion, has been reviewed paying special attention to methods proposed in the literature to determine specific elements or as much as possible components of these products which are widespread used in food preservation or food flavouring and can contribute to the intake of both, essential and toxic elements. Sample treatment and metal speciation aspects have been critically revised concerning onion, garlic and pepper (the most commonly studied products).
Retrotransposon activation by distressed mitochondria in neurons
2020
Retrotransposon activation occurs in a variety of neurological disorders including multiple sclerosis and Alzheimer's Disease. While the origins of disease-related retrotransposon activation have remained mostly unidentified, this phenomenon may well contribute to disease progression by inducing inflammation, disrupting transcription and, potentially, genomic insertion. Here, we report that the inhibition of mitochondrial respiratory chain complex I by pharmacological agents widely used to model Parkinson's disease leads to a significant increase in expression of the ORF1 protein of the long interspersed nucleotide element 1 (LINE1) retrotransposon in human dopaminergic LUHMES cells. These …
Humusvielu izcelsmes – īpašību izpēte izmantojot to multiparametru raksturojumu
2019
Humusvielu izcelsmes-īpašību izpēte izmantojot to multiparametru raksturojumu. Upska K., zinātniskais vadītājs prof., Dr. habil. chem. Kļaviņš M. prof. Dr.chem. Vīksna A. un, Kursa darbs, 62 lappuses, 22 attēli, 11 tabulas, 37 literatūras avoti, 2 pielikumi. Latviešu valodā. Bakalaura darbā tika apkopota zinātniskā literatūra par humusvielām, to izcelsmi, funkcijām biosfērā, ražošanas, izdalīšanas un pētīšanas metodēm. Tika noteikts humusvielu organiskais un neorganiskais elementsastāvs, kas sniedz informāciju par rūpnieciski ražotu humusvielas produktu kvalitāti. Izmantojot UV-Vis un FTIR molekulārās absorbcijas metodi tika uzņemti un analizēti humusvielu absorbcijas spektri. Izmantojot fl…
Potential Functional Significance of Brain-Type and Muscle-Type Nitric Oxide Synthase I Expressed in Adventitia and Media of Rat Aorta
1999
Abstract —Skeletal muscle and myocardium express μNOS I, an elongated splice variant of neuronal-type nitric oxide (NO) synthase (NOS I), and NOS III, endothelial-type NO synthase, respectively. This study was designed to elucidate whether vascular smooth muscle also contains a constitutively expressed NO synthase isoform. In the rat, μNOS I contains an insert of 102 nucleotides after nucleotide 2865 of the cDNA, yielding a protein of 164 kd. Reverse transcription-polymerase chain reaction with primers flanking this insert and with insert-specific primers indicated that endothelium-denuded rat aorta expresses both brain-type NOS I and μNOS I. RNase protection analyses with an antisense RNA…
Whole-epigenome analysis in multiple myeloma reveals DNA hypermethylation of B cell-specific enhancers
2015
Abstract Analyzing the DNA methylome of multiple myeloma (MM), a plasma cell neoplasm, by whole-genome bisulfite sequencing and high-density arrays, we observed regional DNA hypermethylation embedded in extensive global hypomethylation. In contrast to the widely reported DNA hypermethylation of promoter-associated CpG islands (CGIs) in cancer, hypermethylated sites in MM as compared to normal plasma cells were located outside CpG islands and were unexpectedly associated with intronic enhancer regions active in normal B cells. Both RNA-seq and in vitro reporter assays indicated that enhancer hypermethylation is globally associated with downregulation of its host genes. ChIP-seq and DNAseI-se…
A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells.
2017
Principal epididymal cells have one of the largest and more developed Golgi complex of mammalian cells. In the present study, we have used this cell as model for the study of the three-dimensional architecture of the Golgi complex of highly secretory and endocytic cells. Electron tomography demonstrated the presence in this cell type of some unknown or very unusual Golgi structures such as branched cisternae, pocket-like cisternal invaginations or tubular connections. In addition, we have used this methodology and immunoelectron microscopy to analyze the close relationship between this organelle and both the endoplasmic reticulum and microtubules, and to describe in detail how these element…
Comparative Analysis of Four New Alternative Types of Roundabouts: “Turbo”, “Flower”, “Target” and “Four-Flyover” Roundabou
2016
Analysis of literature shows that “modern roundabouts” nowadays exist in all European countries, as well as in more than 60 countries elsewhere in the world. Nowadays, a growing number of studies, presented in scientific and professional literature, point out a poor traffic safety characteristics of “standard” two-lane roundabouts and lower capacity then was expected. These problems are resolved in more ways in different countries; however the solution, whereby the number of conflict spots is diminished has proven to be the most successful. Lower number of conflict spots is one of characteristics of the alternative types of roundabouts. The alternative types of roundabouts are usually more …