Search results for "epitope"
showing 10 items of 455 documents
Hierarchical Imprinting Using Crude Solid Phase Peptide Synthesis Products as Templates
2003
The crude products resulting from solid-phase peptide synthesis can be used as epitope templates to generate surface-confined sites for the template and larger peptides containing the template motif. This offers a facile route to robust affinity stationary phases for the chromatographic separation of peptides.
Deletion and insertion mutants of HBsAg particles
1992
We have found previously that hybrid 22-nm HBsAg particles can be created by insertion of short antigenic sequences into the HBV major envelope protein [1]. We have now performed a detailed deletion mutagenesis of the S gene of HBV encoding HBsAg. Deletion of the 51 C-terminal amino acids including most of the third and all of the fourth hydrophobic domain of the S protein did not affect particle assembly and secretion. However, secretion of 22-nm particles was abolished by minor deletions in the N-terminal region. Insertion and deletion/substitution mutants carrying a poliovirus epitope at the N-terminus and the preSl region at the C-terminus have been characterized.
Glycoconjugate expression and cartilage development of the cranial skeleton.
1998
Only few detailed investigations have focused on the glycobiology of cranial development. The functional elements in most inductive and morphogenetic processes are not individual cells, but rather collectives of interacting populations and extracellular matrix components that give rise to specific tissues and organs. Experimental evidence strongly suggests that sugar chains not only confer morphological characteristics. Complex carbohydrate molecules and their corresponding receptors are involved in recognition processes decoding biological information during cranial morphogenesis. The distribution patterns of glycoconjugates are highly dynamic and show a clear correlation with characterist…
Structural dissection of the multidomain kininogens. Fine mapping of the target epitopes of antibodies interfering with their functional properties.
1993
Kininogens, the large precursor molecules of the vasoactive kinin peptides, are prototypic multidomain proteins serving numerous functions. To investigate their structure-function relationships, we have raised a panel of monoclonal antibodies against human H-kininogen and L-kininogen and fragments thereof and characterized them with respect to their target epitopes. Of 35 antibodies, 12 were directed to the amino-terminal domains (D1 to D3) of cystatin-like structure, 3 recognized domain D4 bearing the kinin segment, 17 bound to the carboxyl-terminal domains of H-kininogen (D5H and D6H), and 3 bound to the carboxyl-terminal domain D5L of L-kininogen. At least 14 distinct epitopes spread ove…
Occurrence of glycine in the core oligosaccharides of Hafnia alvei lipopolysaccharides--identification of disubstituted glycoform.
2015
Endotoxins (lipopolysaccharides, LPS) are the main surface antigens and virulence factors of Gram-negative bacteria involved for example in the development of nosocomial infections and sepsis. They consist of three main regions: O-specific polysaccharide, core oligosaccharide, and lipid A. Bacteria modify LPS structure to escape the immune defence, but also to adapt to environmental conditions. LPS's structures are highly diversified in the O-specific polysaccharide region to evade bactericidal factors of immune system, but retain some common epitopes that are potential candidates for therapeutic strategies against bacterial infections. Common occurrence of glycine within the structure of L…
[1] Neoglycoproteins from synthetic glycopeptides
1994
Publisher Summary Saccharide side chains of glycoproteins influence the physicochemical properties of the biomacromolecules and their stability against proteolytic degradation. Saccharide side chains of glycoproteins also play important roles as ligands in biological recognition and in the organized distribution of these compounds within multicellular organisms. Carbohydrate-lectin interactions are important, for example, in viral infections and for the recruitment and invasion of leukocytes into injured tissues. Although in a number of processes carbohydrates were revealed to be decisive recognition labels, in other biological selections peptide sequences proved to be the recognized areas.…
Towards Adoptive Immunotherapy Using High Affinity T Cell Receptors
2003
The hdm2 oncoprotein is frequently overexpressed in a variety of human malignancies, including acute myeloid and lymphoblastic leukemia. Synthetic peptides representative of hdm2 sequences were tested for their binding to A2.1. HLA-A2.1. (/Kb)-Tg mice were immunized with A2.1-binding hdm2 peptides in order to induce A2.1-restricted and hdm2-specific CTL. A2.1-restricted CTL lines obtained from both A2.1 and HuCD8 × A2.1/Kb-Tg mice were able to recognize a synthetic 8-mer peptide representative of a N-terminal hdm2 sequence. These CTL were capable of recognizing and lysing Saos-2 cells transfected with the hdm2 gene as opposed to the parental cell line that lacks any detectable hdm2 expressi…
Characterization of HLA-DR- and TCR-binding residues of an immunodominant and genetically permissive peptide of the 16-kDa protein of Mycobacterium t…
2004
The 16-kDa protein of Mycobacterium tuberculosis represents an important antigenic target during bacillary latency and, consequently, should be considered as candidate subunit vaccine component. In this study, we have used CD4 T cell clones that recognize the peptide p91-110, an immunodominant and genetically permissive epitope, in the context of five different HLA-DR molecules and truncated and substituted variants of this peptide, to identify the minimal binding sequence (HLA-DR-binding core) and the minimal stimulatory sequence (TCR-binding core), as well as the residues that contact HLA-DR molecules and the TCR. We have found a common 9-mer sequence, spanning amino acids 93-101, as the …
Mapping antigenic epitopes of potato virus Y with antibodies affinity-purified by using overlapping synthetic peptides
1994
Synthetic, overlapping peptides representing the entire amino acid sequence of potato virus Y (PVY) coat protein were used to affinity-purify antibodies from polyclonal antisera to PVY. In testing the binding of the purified antibodies to PVY particles, antigenic epitopes were identified. The N-terminal and C-terminal regions of the PVY coat protein were found to contain most of the antigenic epitopes. The results will facilitate the development of detection methods for PVY based on synthetic peptides.
Pathways and Mechanisms of Human T Cell Activation
1985
The antigen receptor of T lymphocytes was recently identified as a complex consisting of a 90 KD disulfide linked heterodimer, termed Ti which is functionally and structurally associated with three additional molecular components, termed T3 (1). Whereas the former contains clonally unique epitopes and displays peptide variability among T cell clones of distinct specificities, no variability could be detected within any of the known three subunits of T3 (2,3). Monoclonal antibodies to T3 and Ti, respectively, in soluble form were capable of blocking antigen specific clonal T cell responses (4,5). Perhaps more importantly, when coupled to the surface of a solid support these antibodies produc…