Search results for "finger"

showing 10 items of 400 documents

Label-free piezoelectric biosensor for prognosis and diagnosis of Systemic Lupus Erythematosus

2017

[EN] An autoantigen piezoelectric sensor to quantify specific circulating autoantibodies in human serum is developed. The sensor consisted on a quartz crystal microbalance with dissipation monitoring (QCM-D) where TRIM21 and TROVE2 autoantigens were covalently immobilized, allowing the selective determination of autoantibodies for diagnosis and prognosis of Systemic Lupus Erythematosus (SLE). The sensitivity of the biosensor, measured as IC50 value, was 1.51 U/mL and 0.32 U/mL, for anti-TRIM21 and anti-TROVE2 circulating autoantibodies, respectively. The sensor is also able to establish a structural interaction fingerprint pattern or profile of circulating autoantibodies, what allows scorin…

Biomedical EngineeringBiophysicsEarly detectionBiosensing Techniques02 engineering and technologyImmunosensorDissipation monitoringAutoantigensSensitivity and SpecificitySystemic Lupus Erythematosus01 natural sciencesQuartz crystal microbalanceRNA Small CytoplasmicDiagnosisQUIMICA ANALITICAElectrochemistryHumansLupus Erythematosus SystemicMedicineMultiplexPiezoelectric biosensorAutoantibodiesLabel freeRibonucleoproteinbusiness.industry010401 analytical chemistryAutoantibodyGeneral MedicineQuartz crystal microbalancePrognosis021001 nanoscience & nanotechnology0104 chemical sciencesInteraction fingerprintRibonucleoproteinsImmunologyQuartz Crystal Microbalance Techniques0210 nano-technologybusinessBiosensorBiotechnologyBiosensors and Bioelectronics
researchProduct

Unusual Neuroendocrine Differentiation in a Small Round Cell Angiosarcoma: A Potential Histologic Mimicker of Superficial Ewing Sarcoma.

2018

Neuroendocrine differentiation or aberrant expression of neuroendocrine markers is very uncommon in angiosarcomas (AS) and creates a challenging differential diagnosis with other superficial or soft tissue tumors. Herein, we report a new case of superficial AS presenting as a tumor lesion on the little finger of the right hand of a 52-year-old man. The tumor displayed CD56, chromogranin-A, and synaptophysin immunoreactivity. Tumor cells were positive for vascular markers (CD31, FLI1, ERG, D2-40, VE-cadherin, VEGR1,2, and 3), CD99, and EMA, but were negative for S100, CK (AE1/AE3), CK20, polyomavirus, and myogenic (desmin and myogenin) and melanocyte markers (melan-A and HMB45). Ki67 immunos…

CD31MalePathologymedicine.medical_specialtySkin NeoplasmsBiopsyCD99HemangiosarcomaDermatologySarcoma EwingNeuroendocrine differentiationPathology and Forensic MedicineDiagnosis DifferentialFingers030207 dermatology & venereal diseases03 medical and health sciences0302 clinical medicinePredictive Value of TestsBiopsymedicineBiomarkers TumorHumansAngiosarcomaIn Situ Hybridization FluorescenceCell Proliferationbiologymedicine.diagnostic_testMerkel cell carcinomabusiness.industryCell DifferentiationGeneral MedicineMiddle Agedmedicine.diseaseImmunohistochemistryCarcinoma Neuroendocrine030220 oncology & carcinogenesisSarcoma Small CellSynaptophysinbiology.proteinSarcomabusinessThe American Journal of dermatopathology
researchProduct

Stronger proprioceptive BOLD-responses in the somatosensory cortices reflect worse sensorimotor function in adolescents with and without cerebral pal…

2020

Graphical abstract

CP-oireyhtymäCHILDRENSM1PASSIVE FINGERDP diplegic3124 Neurology and psychiatryEVOKED-POTENTIALSBRAINChildMOTOR CORTEXPassive movementTE echo timeEM expectation maximizationliikeaistiBOLD Blood-Oxygen-Level-Dependent signalRegular ArticleMagnetic Resonance ImagingTD typically-developedTR repetition timeSIIGMFCS Gross Motor Function Classification SystemMANCOVA Multivariate analysis of covarianceEPI echo planar imagingHP hemiplegicfMRI functional magnetic resonance imagingFemaleTACTILE STIMULATIONhalvausAGE-RELATED DIFFERENCESAdolescentComputer applications to medicine. Medical informaticsR858-859.7HemiplegiaORGANIZATIONDiplegiatuntoaistiMOVEMENTSIPT Sensory Integration and Praxis TestsROI regions of interestHumansSISII cortex secondary somatosensory cortexCP cerebral palsyRC346-429ComputingMethodologies_COMPUTERGRAPHICSGLM General Linear ModelCerebral Palsy3112 NeurosciencesSPM Statistical Parametric MappingSomatosensory CortexHandProprioceptionSI cortex primary somatosensory cortexGABA CONCENTRATIONKinesthesiaNeurology. Diseases of the nervous systemPSC percent signal change
researchProduct

Chemometrics as a Tool of Origin Determination of Polish Monofloral and Multifloral Honeys

2014

The aim of this study was to evaluate the application of chemometrics studies to determine the botanical origin of Polish monofloral honeys using NMR spectroscopy. Aqueous extracts of six kinds of honeys, namely, heather (Calluna vulgaris L.), buckwheat (Fagopyrum esculentum L), lime (Tilia L), rape (Brassica napus L. var. napus), acacia (Acacia Mill.), and multifloral ones, were analyzed. Multivariate chemometric data analysis was performed using principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). Chemometric analysis supported by pollen analysis revealed the incorrect classification of acacia honeys by the producers. Characteristic moti…

CallunaMagnetic Resonance Spectroscopychemical profilefloral markersAcaciaFlowersmedicine.disease_causechemical fingerprintingChemometricsTiliaPollenBotanymedicine1H NMR spectroscopyorigin of honeyPrincipal Component AnalysisPCAbiologyChemistryDiscriminant AnalysisHoneyGeneral Chemistrychemometricsbiology.organism_classificationOPLS-DAPrincipal component analysisPolandGeneral Agricultural and Biological SciencesChemical fingerprintingFagopyrumJournal of Agricultural and Food Chemistry
researchProduct

A new pericentric inv(8) in acute nonlymphocytic leukemia.

1993

Abstract Chromosome studies were carried out on unstimulated bone marrow cells from a patient with a diagnosis of acute nonlymphocytic leukemia (FAB M6 ANLL). Cytogenetic analysis revealed a mosaic chromosome pattern 46,XX46,XX,inv(8)(p21q24). This pericentric inversion has not been previously described in ANLL. Because, fragile sites, zinc finger gene loci, and the MYC protooncogene have been localized to band 8q24, a putative role for these sites and genes could be considered.

Cancer ResearchBiologychromosome Acute nonlymphocytic leukemia cytogeneticshemic and lymphatic diseasesGeneticsmedicineHumansMolecular BiologyGeneChromosomal inversionZinc fingerGeneticsChromosomal fragile siteChromosomeMiddle Agedmedicine.diseaseMolecular biologySettore BIO/18 - GeneticaLeukemiaLeukemia Myeloid Acutemedicine.anatomical_structureKaryotypingChromosome InversionFemaleBone marrowChromosomes Human Pair 8Cancer genetics and cytogenetics
researchProduct

The Muscleblind family of proteins: an emerging class of regulators of developmentally programmed alternative splicing.

2006

Alternative splicing is widely used to generate protein diversity and to control gene expression in many biological processes, including cell fate determination and apoptosis. In this review, we focus on the Muscleblind family of tissue-specific alternative splicing regulators. Muscleblind proteins bind pre-mRNA through an evolutionarily conserved tandem CCCH zinc finger domain. Human Muscleblind homologs MBNL1, MBNL2 and MBNL3 promote inclusion or exclusion of specific exons on different pre-mRNAs by antagonizing the activity of CUG-BP and ETR-3-like factors (CELF proteins) bound to distinct intronic sites. The relative activities of Muscleblind and CELF proteins control a key developmenta…

Cancer ResearchCellular differentiationMolecular Sequence DataRNA-binding proteinCell fate determinationBiologychemistry.chemical_compoundExonMiceMBNL1AnimalsHumansMyotonic DystrophyAmino Acid SequenceMolecular BiologyGeneticsZinc fingerAlternative splicingGene Expression Regulation DevelopmentalRNA-Binding ProteinsCell DifferentiationZinc FingersCell BiologyAlternative SplicingchemistryRNA splicingDevelopmental BiologyDifferentiation; research in biological diversity
researchProduct

3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

2013

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).

Chemical imagingmedicine.medical_specialtyMaterials scienceInfrared spectroscopyBiochemistryFourier transform spectroscopyPhysics::GeophysicsMicesymbols.namesakeImaging Three-DimensionalOpticsSpectroscopy Fourier Transform InfraredMicroscopyImage Processing Computer-AssistedmedicineAnimalsHumansFourier transform infrared spectroscopyMolecular BiologyEmbryonic Stem Cellsbusiness.industryX-Ray MicrotomographyCell BiologyWoodSpectral imagingPopulusFourier transformsymbolsbusinessChemical fingerprintingSynchrotronsHairBiotechnologyNature Methods
researchProduct

Fingerprints from fingerprints

2003

Besides ‘‘classical’’ biological materials such as blood and sperm, epithelial cells from latent fingerprints are targeted in forensic sciences. In addition to studies using latent fingerprints applied to beer glasses [1], T-shirts left on crime scenes [2] and various other objects [3], we report the detection of STR profiles from latent fingerprints deposited on ordinary sheets of paper. In contrast to the relatively high number of epithelial cells from saliva or from excessively pressured fingerprints during strangulation [4,5], the experiments with latent fingerprints are expected to generate only a very small number of epithelial cells. Moreover, cells remaining on objects touched only …

Chemistrybusiness.industryFingerprint (computing)Pattern recognitionGeneral MedicineArtificial intelligencebusinessBiological materialsInternational Congress Series
researchProduct

Comparative analysis of short tandem repeats and single nucleotide polymorphisms on the Y-chromosome in Germans, Chinese and Thais.

2003

We have typed genomic DNA samples from 95 individuals from Western Germany, 78 individuals from Bangkok/Thailand and 56 individuals from Chengdu/China for 11 Y-chromosomal diallelic polymorphisms and eight short tandem repeat (STR) systems. For single nucleotide polymorphism (SNP) analysis, a rapid method was applied using the single base extension technology (minisequencing) in combination with capillary electrophoresis. PCR products for SRY-8299, Tat, SRY2627, 92R7, SRY1532, M9, M13, M17/M19 and M20 were pooled and used as templates for the commercially available SNaPshot kit. In addition to these ten SNPs we also tested the Y-chromosomal diallelic Alu repeat insertion DYS287 (YAP) by aga…

ChinaSTR multiplex systemPopulationSingle-nucleotide polymorphismBiologyPolymerase Chain ReactionHaplogroupPathology and Forensic MedicineGene FrequencyGermanyEthnicityHumanseducationGeneticsElectrophoresis Agar Geleducation.field_of_studyChromosomes Human YPolymorphism GeneticHaplotypeElectrophoresis Capillarysocial sciencesSingle-base extensionThailandDNA Fingerprintingeye diseaseshumanitiesIssues ethics and legal aspectsSTR analysisHaplotypesTandem Repeat SequencesMicrosatellitegeographic locationsLegal medicine (Tokyo, Japan)
researchProduct

Botanical Origin Authentication of Polish Phacelia Honey Using the Combination of Volatile Fraction Profiling by HS-SPME and Lipophilic Fraction Prof…

2019

Eleven samples of Polish Phacelia tanacetifolia Benth., three Brassica napus and one Salix spp. honeys were characterized by melissopalynology and analysis of the compositions of their volatile fractions. Headspace solid-phase microextraction coupled with gas chromatography mass spectrometry (HS-SPME/GC–MS) using PDMS/CAR/DVB fiber was used for the isolation of low-molecular weight compounds which create a volatile fraction. To differentiate and indicate the most representative unifloral samples, chemometric techniques such as principal component analysis (PCA) and hierarchical-tree clustering (HTC) were applied to the dataset of the chromatographic fingerprints. Based on the obtained resul…

Chromatographybiology010405 organic chemistryChemistry010401 analytical chemistryOrganic ChemistryClinical BiochemistryFingerprintHoneyMass spectrometrybiology.organism_classification01 natural sciencesBiochemistryHoney samples0104 chemical sciencesAnalytical ChemistryHPTLCPhacelia tanacetifoliaMelissopalynologyPhaceliaPrincipal component analysisHS-SPMEGas chromatography–mass spectrometryChemical compositionFood qualityChromatographia
researchProduct