Search results for "fluor"
showing 10 items of 7252 documents
NANO-EMETTITORI NIR A BASE DI SILICE PER APPLICAZIONI IN-VIVO E RELATIVO PROCESSO DI PRODUZIONE
2010
Realizzazione di nano-sonde paramagnetiche e fosforescenti, ottenibili a partire da particelle nanometriche di silice mediante procedure di sintesi e arricchimento di O2 a seguito di opportuni trattamenti termici in ambiente controllato. Le nano-sonde così ottenute presentano emissioni nel vicino infrarosso (NIR), sono eccitabili nel visibile e nel NIR, e sono caratterizzate da un tempo di vita nell’ordine del secondo. Tali nano-sonde presentano un notevole potenziale scientifico e commerciale nel mercato della spettroscopia confocale per bio-imaging e nel mercato delle applicazioni medico-farmaceutiche di drug-labelling e drug-delivery.
Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine
2010
Jonna Nykky, Jenni E Tuusa, Sanna Kirjavainen, Matti Vuento, Leona GilbertNanoscience Center and Department of Biological and Environmental Science, University of Jyväskylä, FinlandAbstract: Viruses have great potential as nanotools in medicine for gene transfer, targeted gene delivery, and oncolytic cancer virotherapy. Here we have studied cell death mechanisms of canine parvovirus (CPV) to increase the knowledge on the CPV life cycle in order to facilitate the development of better parvovirus vectors. Morphological studies of CPV-infected Norden laboratory feline kidney (NLFK) cells and canine fibroma cells (A72) displayed characteristic apoptotic events. Apoptosis was f…
SENSING OF AMYLOID AGGREGATION USING A NOVEL BIS-THIOPHENE FLUORESCENT DYE
2012
Diels–Alder reaction of β-fluoro-β-nitrostyrenes with cyclic dienes
2021
The Diels–Alder reaction of β-fluoro-β-nitrostyrenes with cyclic 1,3-dienes was investigated. A series of novel monofluorinated norbornenes was prepared in up to 97% yield. The reaction with 1,3-cyclohexadiene permits the preparation of monofluorinated bicyclo[2.2.2]oct-2-enes. The kinetic data of the reactions with 1,3-cyclopentadiene and 1,3-cyclohexadiene were used to calculate activation parameters. Furthermore, the synthetic utility of the cycloadducts obtained was demonstrated.
Diurnal Cycle Relationships between Passive Fluorescence, PRI and NPQ of Vegetation in a Controlled Stress Experiment
2017
In order to estimate vegetation photosynthesis from remote sensing observations; some critical parameters need to be quantified. From all absorbed light; the plant needs to release any excess that is not used for photosynthesis; by non-photochemical quenching; by fluorescence emission and unregulated thermal dissipation. Non-photochemical quenching (NPQ) processes are controlled photoprotective mechanisms which; once activated; strongly control the dynamics of photochemical efficiency. With illumination conditions increasing and decreasing during a diurnal cycle; photoprotection mechanisms needs to change accordingly. The goal of this work is to quantify dynamic NPQ; measured from active fl…
Development of functionalized SYBR green II related cyanine dyes for viral RNA detection
2020
Fluorescent probes for sensing nucleic acids have found widespread use in the field of cell and molecular biology. However, probes combined with potential for post-synthetic conjugation, e.g. for intra-endosomal measurements of RNA, are unavailable. Herein we developed cyanine dyes that can be conjugated to viral capsid or other targets. First, we solved the crystal structure of SYBR Green II. The structural elucidation of this commonly used RNA probe provided the basis for synthesizing similar molecules with much desired function for post-synthetic conjugation. To address this need, cyanine dyes were prepared using an alternative synthesis protocol. All studied compounds showed considerabl…
Evaluación de las técnicas de obtención de muestra percutánea transtorácica de lesiones pulmonares y mediastínicas guiadas por Tomografía Computariza…
2023
Las técnicas empleadas para la obtención de muestras de lesiones torácicas, mediante Punción Aspiración con Aguja Fina (PAAF) y Biopsia con Aguja Gruesa (BAG), a partir de punciones percutáneas guiadas por exploraciones radiológicas (con Tomografía Computarizada (TC), fluoroscopia-TC o ecografía), tienen un papel destacado en tres escenarios clínicos concretos: 1) Manejo y diagnóstico del nódulo pulmonar: • Diferenciación benignidad vs malignidad, caracterización anatomopatológica +/- microbiológica. 2) Diagnóstico y estadificación del cáncer de pulmón: • Tipificación anatomopatológica e inmunohistoquímica, realización de estudios moleculares (basales y de resistencia a los tratamientos imp…
Pulsējošā elektromagnētiskā un gaismas starojuma ietekme uz baktēriju dzīvotspēju
2020
Baktēriju augšanu ir iespējams ietekmēt ar elektromagnētisko starojumu. Šī darba mērķis bija pētīt Escherichia coli un Staphylococus aureus kultūru dzīvotspēju pulsējošā elektromagnētiskā un gaismas starojuma ietekmē, vadoties pēc R. R. Raifa izstrādātās mikroorganismu nāvējošās oscilācijas hipotēzes. Šim nolūkam tika replicēta R.R. Raifa elektromagnētiskā starojuma ierīce, kas apstaroja kultūras režīmos no 600 Hz līdz 1000 Hz laika periodā 10 sekundes. Dzīvotspēja tika noteikta, mikroskopējot un skaitot šūnas, kas tika iekrāsotas ar ThermoFisher LIVE/DEAD BacLight Bacterial Viability Kit. Tika noteiktas divas atšķirīgas baktericīdas frekvences – 800 Hz (E.coli) un 727 Hz (S.aureus), ar kur…
Substrate specificity screening of oat (Avena sativa) seeds aminopeptidase demonstrate unusually broad tolerance in S1 pocket.
2012
Aminopeptidases are proteolytic enzymes that remove one amino acid at a time from N-terminus of peptidic substrates. In plants, inhibitors of aminopeptidases can find potential applications in agriculture as herbicides. In this report we have used a library of fluorogenic derivatives of natural and unnatural amino acids for substrate specificity profiling of oat (Avena sativa) aminopeptidase. Interestingly, we have found that this enzyme recognizes effectively among the natural amino acids basic residues like Arg and Lys, hydrophobic Phe, Leu and Met, but also to some extent acidic residues Asp and Glu. In the case of unnatural amino acids hydrophobic residues (hPhe and hCha) and basic hArg…
Solvatochromic behaviour of new donor–acceptor oligothiophenes
2021
Oligothiophene derivatives play a central role in the formulation of materials used in devices in the field of organic electronics. In this work, we report the results of the study of UV-vis absorption and fluorescence spectra, in several solvents, of a series of oligothiophenes recently synthesized in our laboratory. The studied oligothiophenes present different structures due to several factors: the donor– acceptor (D–A) or acceptor–donor–acceptor (A–D–A) architecture, the number of thiophene rings in the backbone (ranging from three to eight), the number and position of solubilizing octyl chains in the backbone, and the type of acceptor moieties (from Knoevenagel condensation either with…