Search results for "focal"
showing 10 items of 803 documents
Comparison of growth & function of endothelial progenitor cells cultured on deproteinized bovine bone modified with covalently bound fibronectin …
2016
Objectives The objective of this study was to assess and compare the growth and function of Endothelial Progenitor Cells (EPCs) cultured on covalently bonded Vascular Endothelial Growth Factor (VEGF) and covalently bonded Fibronectin (FN) coating on deproteinized bovine bone (DBB) (test samples), compared to non-modified DBB blocks (control sample). Materials and methods The test samples were prepared by plasma polymerization of allylamine onto DBB blocks. Group1 of test samples were prepared with VEGF coating (VEGF-DBB) where as the Group2 test samples were coated with FN (FN-DBB). Non-modified DBB blocks served as a Control. EPCs were isolated and cultivated from buffy coats of peripheral…
Molecular imaging of VEGF in gastrointestinal cancer in vivo using confocal laser endomicroscopy
2010
Vascular endothelial growth factor (VEGF) is a therapeutic target in gastrointestinal cancer (GiC). However, its in vivo visualisation could not be achieved to date with endoscopic techniques. Confocal laser endomicroscopy (CLE) is a novel imaging technique for gastrointestinal endoscopy providing in vivo microscopy at subcellular resolution. The aim of the study was to evaluate CLE for in vivo molecular imaging of VEGF in GiC.Molecular imaging of tumours in APCmin mice, in xenograft models and in surgical specimens of patients with colorectal cancer (CRC) was achieved after application of labelled antibodies. The tumour sites were scanned with the probe for the strongest specific fluoresce…
Agonist and antagonist-dependent internalization of the human vasopressin V2 receptor.
1998
Abstract In this report we demonstrate that in HEK293 cells stably expressing the human V2vasopressin receptor, ligand-induced internalization of the hormone receptor occurs via the clathrin-dependent pathway. Studies of receptor trafficking either by direct visualization of the V2receptor by confocal microscopy or binding experiments show a rapid internalization (half-time 6–7 min). Blocking of the clathrin-dependent pathway by hypertonic sucrose increased vasopressin-induced cellular cAMP production and decreased the desensitization of the V2receptor–adenylyl cyclase system. Thus, internalization appears to be a major regulatory mechanism terminating vasopressin action in HEK293 cells. Tw…
The Assessment of Diffuse Disease
2016
A significant drop in pressure can be observed also in the absence of focal lesions. The presence of hemodynamically relevant, diffuse atherosclerosis is actually very frequent and it may affect as many as 50 % of those patients who have at least one focal lesion in another vessel. FFR in this setting may clarify the possible reason for residual angina after successful stenting.
Visual performance with multifocal intraocular lenses
2004
Abstract Objective To evaluate distance and near visual performance under bright (photopic) and dim (mesopic) conditions in patients who had undergone uncomplicated cataract extraction with multifocal or monofocal intraocular lens (IOL) implantation. Design Prospective, nonrandomized, masked, comparative, observational case series. Participants Thirty-two eyes of 32 patients after zonal-progressive multifocal IOL implantation (Allergan Medical Optics Array SA-40N) and 32 eyes of 32 age-matched patients after monofocal IOL implantation (Allergan Medical Optics SI-40NB). Intervention All eyes underwent phacoemulsification and IOL implantation. Main outcome measures At 18 months after surgery,…
Objective and subjective evaluation of photic phenomena after monofocal and multifocal intraocular lens implantation11The authors have no commercial …
1999
Abstract Objective To objectively measure and compare halo, flicker, and glare disability in pseudophakic eyes with monofocal (MONO) and multifocal (MULTI) intraocular lenses (IOLs) with respect to the influence of corneal surface quality, astigmatism, and age. Design Prospective case series. Participants This clinical trial involved 28 eyes of 28 patients after small-incision cataract surgery with a MONO silicone IOL and 28 eyes of 28 patients with zonal-progressive silicone IOL. Intervention A computer program objectively determined halo, glare, and flicker. Corneal surface quality and astigmatism were measured using computerized videokeratography. Ray-tracing analysis was performed based…
In vitro optical performance of nonrotational symmetric and refractive-diffractive aspheric multifocal intraocular lenses: impact of tilt and decentr…
2012
Purpose To evaluate the optical quality of Acrysof Restor SN6AD1 refractive–diffractive +3.00 D and Lentis Mplus LS-312 nonrotational symmetric +3.00 D aspheric multifocal intraocular lenses (IOLs) and analyze the effect of tilt and decentration. Setting University of Valencia, Valencia, and University of Murcia, Murcia, Spain. Design Experimental study. Methods An artificial model eye was used to measure wavefront aberrations of both IOL models. The modulation transfer function (MTF) and point-spread function (PSF) were calculated for a 5.0 mm pupil and under 5 situations: centered, 0.2 mm and 0.4 mm decentered, and 2 degrees and 4 degrees tilted. Results The refractive–diffractive IOL had…
Thin plastic foil X-ray optics with spiral geometry
2007
Winding a plastic foil ribbon into spiral cylinder or spiral cones we can design and build single or multiple reflection X-ray grazing incidence focusing optics with potential applications in Astronomy as well as experimental physics. The use of thin plastic foils from common industrial applications and of a mounting technique which does not require the construction of mandrels make these optics very cost effective. A spiral geometry focusing optic produces an annular image of a point source with the angular size of the annulus depending mainly on the pitch of the winding and the focal length. We use a ray-tracing code to evaluate the performances of cylindrical, and double conical spiral g…
Increased electron donor and electron acceptor characters enhance the adhesion between oil droplets and cells of Yarrowia lipolytica as evaluated by …
2003
The adhesion of methyl ricinoleate droplets to cells of the yeast Yarrowia lipolytica was investigated. A new cytometric method, relying on the double staining of fatty globules with Nile Red and of cells with Calcofluor, enabled us to quantify methyl ricinoleate droplet adhesion to cells precultured on a hydrophilic or on a hydrophobic carbon source. In this last case, droplet adsorption was enhanced and a MATS (microbial adhesion to solvents) test revealed that this increase was due to Lewis acid-base interactions and not to an increase in the hydrophobic properties of the cell surface. These preliminary results demonstrate that the developed cytometric method is promising for various app…
Influence of fluorine on the fiber performance studied through the NBOHC-related 1.9 eV microluminescence
2012
International audience; The distribution of Non Bridging Oxygen Hole Centers (NBOHC) in Fluorine doped optical fibers was investigated by confocal microluminescence spectroscopy monitoring the characteristic 1.9 eV luminescence band. The results show that these defects are generated by the fiber drawing and their concentration further increases after γ irradiation. The NBOHC profile along the fiber is anticorrelated to the fluorine content. This finding agrees with the role of fluorine in the fiber toughness and is discussed from the microscopic point of view on the basis of previous works.