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showing 10 items of 2099 documents

Enhanced levels of S-linalool by metabolic engineering of the terpenoid pathway in spike lavender leaves

2013

Transgenic Lavandula latifolia plants overexpressing the linalool synthase (LIS) gene from Clarkia breweri, encoding the LIS enzyme that catalyzes the synthesis of linalool were generated. Most of these plants increased significantly their linalool content as compared to controls, especially in the youngest leaves, where a linalool increase up to a 1000% was observed. The phenotype of increased linalool content observed in young leaves was maintained in those T1 progenies that inherit the LIS transgene, although this phenotype was less evident in the flower essential oil. Cross-pollination of transgenic spike lavender plants allowed the generation of double transgenic plants containing the …

LavenderAcyclic MonoterpenesLavandulaTransgeneLavandula latifoliaBioengineeringGenetically modified cropsClarkiaBiologyApplied Microbiology and Biotechnologylaw.inventionchemistry.chemical_compoundLinaloollawBotanyTransgenesHydro-LyasesEssential oilPlant ProteinsPlants Genetically Modifiedbiology.organism_classificationTerpenoidPlant LeavesErythritolLavandulachemistryMonoterpenesSugar PhosphatesBiotechnologyMetabolic Engineering
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The Eyjafjallajokull volcanic summit eruption: evidences from ash-leachates analysis and ground deposition fluxes

2012

The Eyjafjallajokull 2010 eruption was an extraordinary event in that it led to widespread over Europe. Volcanic processes which lead to eruptions can be investigated by monitoring a variety of parameters, including the composition of ash leachates. Fine-grained tephra erupted from active vents, and transported through volcanic plumes, can adsorbs, and therefore rapidly scavenge, volatile elements such as S, halogens, and metal species in the form of soluble salts adhering to ash surfaces. Analysis of such water-soluble phases is a suitable complement for the remote sensing of volcanic gases at inaccessible volcanoes, like Eyjafjallajokull. The 2010 Eyjafjallajokull eruption developed in fo…

Leachate ash Eyjafjallajokull 2010 eruptionSettore GEO/08 - Geochimica E Vulcanologia
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Raman spectroscopic study of some lead phosphate glasses with tungsten ions

2006

Abstract The structure of x WO 3  · (100 −  x )[2P 2 O 5  · PbO] glass system with 0 ⩽  x  ⩽ 50 mol% was investigated by Raman spectroscopy. The characteristic bands of these glasses due to the stretching and bending vibrations were identified and analyzed by the increasing of WO 3 content. This fact allowed us to identify the specific structural units which appear in these glasses and thus to point out the network modifier role of tungsten oxide for low concentrations and its former role at high concentrations.

Lead phosphateChemistryInorganic chemistryAnalytical chemistrychemistry.chemical_elementTungstenCondensed Matter PhysicsElectronic Optical and Magnetic MaterialsIonPhosphate glasssymbols.namesakeChemical bondMolecular vibrationMaterials ChemistryCeramics and CompositessymbolsRaman spectroscopyChemical compositionJournal of Non-Crystalline Solids
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A new N6 hexadentate ligand and a novel heptacoordinated N6O-type Fe(III) compounds: Synthesis, characterization and structure of [Fe(dimpyen)(OH)](A…

2011

Abstract In this contribution, we report the syntheses of a novel N6 donor set ligand, dimpyen = N1,N2-di[(1-methyl-1H-2-imidazolyl) methyl]-N1,N2-di(2-pyridilmethyl)-1,2-ethanediamine. This type of ligand was designed to modulate the properties of the metal ions bound to it. The reaction with Fe(II) gives off a new heptacoordinated iron(III) complex. We study the spectroscopic (UV–Vis), magnetic and electrochemical behavior and also made the structural determination with X-ray diffraction at 134 K and a heptacoordinated N6O-type derivative of Fe(III) is reported as well. This complex crystallize as perchlorate or hexafluorophosphate and the formula of these derivatives are [Fe(dimpyen)(OH)…

LigandMetal ions in aqueous solutionInorganic chemistryCrystal structureElectrochemistryInorganic Chemistrychemistry.chemical_compoundPerchlorateCrystallographyPentagonal bipyramidal molecular geometrychemistryOctahedronHexafluorophosphateMaterials ChemistryPhysical and Theoretical ChemistryInorganica Chimica Acta
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The minimal model of Hahn for the Calvin cycle.

2018

There are many models of the Calvin cycle of photosynthesis in the literature. When investigating the dynamics of these models one strategy is to look at the simplest possible models in order to get the most detailed insights. We investigate a minimal model of the Calvin cycle introduced by Hahn while he was pursuing this strategy. In a variant of the model not including photorespiration it is shown that there exists exactly one positive steady state and that this steady state is unstable. For generic initial data either all concentrations tend to infinity at lates times or all concentrations tend to zero at late times. In a variant including photorespiration it is shown that for suitable v…

LightExistential quantificationMolecular Networks (q-bio.MN)02 engineering and technologyDynamical Systems (math.DS)Mathematical proofBiochemistryModels BiologicalMinimal modelsymbols.namesakeAdenosine Triphosphate0502 economics and business0202 electrical engineering electronic engineering information engineeringFOS: MathematicsApplied mathematicsQuantitative Biology - Molecular NetworksMathematics - Dynamical SystemsPhotosynthesisMathematicsCompactification (physics)Applied Mathematics05 social sciencesGeneral MedicineCarbon DioxideOxygenComputational MathematicsKineticsGlucoseModeling and SimulationFOS: Biological sciencesPoincaré conjecturesymbols020201 artificial intelligence & image processingGeneral Agricultural and Biological Sciences92C40 34C60050203 business & managementAlgorithmsMathematical biosciences and engineering : MBE
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Photoaffinity Labeling and Photoaffinity Cross-Linking of Phosphofructokinase-1 from Saccharomyces cerevisiae by 8-Azidoadeninenucleotides

2001

Phosphofructokinase-1 from Saccharomyces cerevisiae is composed of four alpha- and four beta-subunits, each of them carrying catalytic and regulatory bindings sites for MgATP. In this paper, various photoaffinity labels, such as 8-azidoadenosine 5'-triphosphate, 8-azido-1,N6-ethenoadenosine 5'-triphosphate, and 8-N3-3'(2')-O-biotinyl-8-azidoadenosine 5'-triphosphate have been used to study their interaction with the enzyme in the dark and during irradiation. All nucleotidetriphosphates function as phosphate donor forming fructose 1,6-bisphosphate from fructose 6-phosphate. However, the kinetic analysis revealed distinctly differences between them. Photolabeling causes a decrease in enzyme a…

LightPhosphofructokinase-1Blotting WesternSaccharomyces cerevisiaeBiophysicsPhotoaffinity LabelsSaccharomyces cerevisiaePhotoaffinity LabelsBiochemistryAdenosine TriphosphateFructosediphosphatesChymotrypsinMagnesiumPhosphofructokinase 1Molecular Biologychemistry.chemical_classificationGel electrophoresisBinding SitesAffinity labelingbiologyPhotoaffinity labelingFructosephosphatesDarknessbiology.organism_classificationEnzyme assayKineticsProtein SubunitsCross-Linking ReagentsEnzymechemistryBiochemistrybiology.proteinElectrophoresis Polyacrylamide GelArchives of Biochemistry and Biophysics
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Energy status and its control on embryogenesis of legumes: ATP distribution within Vicia faba embryos is developmentally regulated and correlated wit…

2003

To analyse the energy status of Vicia faba embryos in relation to differentiation processes, we measured ATP concentrations directly in cryosections using a quantitative bioluminescence-based imaging technique. This method provides a quantitative picture of the ATP distribution close to the in vivo situation. ATP concentrations were always highest within the axis. In pre-storage cotyledons, the level was low, but it increased strongly in the course of further development, starting from the abaxial region of cotyledons and moving towards the interior. Greening pattern, chlorophyll distribution and photosynthetic O2 production within embryos temporally and spatially corresponded to the ATP di…

LightPhotoperiodPlant ScienceBiologyPhotosynthesischemistry.chemical_compoundAdenosine TriphosphateGreeningBotanyGeneticsStorage proteinPhotosynthesischemistry.chemical_classificationfood and beveragesEmbryoCell BiologyDarknessPhotosynthetic capacityVicia fabaVicia fabaCell biologyOxygenchemistryChlorophyllSteady state (chemistry)Energy MetabolismThe Plant Journal
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Color change of the bleached enamel treated with calcium silicate- sodium phosphate-sodium monofluorophosphate-based system

2018

Background The increasing demand of tooth bleaching has also increased the need of researches focusing on the durability of the resultant color. The aim of this study was to evaluate the effect of a system based on calcium silicate, sodium phosphate and sodium monofluorophosphate (Regenerate™) on color maintenance of bleached enamel considering two waiting times for the contact with a cola drink. Material and Methods This in vitrostudy was performed on bovine enamel specimens (n = 100), bleached with 35% H2O2 and treated with: G1 Distilled water; G2 Artificial saliva; G3 RegenerateTM Serum and Toothpaste; G4 RegenerateTM Toothpaste; G5 RegenerateTM Serum. The groups G3, G4 and G5 received o…

LightnessToothpastebusiness.product_categoryEnamel paintgenetic structuresResearchSodiumchemistry.chemical_elementEsthetic DentistrySodium monofluorophosphate:CIENCIAS MÉDICAS [UNESCO]chemistry.chemical_compoundchemistryDistilled watervisual_artCalcium silicateUNESCO::CIENCIAS MÉDICASvisual_art.visual_art_mediumFood scienceHydrogen peroxidebusinessGeneral Dentistry
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The use of cultured hepatocytes to investigate the metabolism of drugs and mechanisms of drug hepatotoxicity.

2001

Hepatotoxins can be classified as intrinsic when they exert their effects on all individuals in a dose-dependent manner, and as idiosyncratic when their effects are the consequence of an abnormal metabolism of the drug by susceptible individuals (metabolic idiosyncrasy) or of an immune-mediated injury to hepatocytes (allergic hepatitis). Some xenobiotics are electrophilic, and others are biotransformed by the liver into highly reactive metabolites that are usually more toxic than the parent compound. This activation process is the key to many hepatotoxic phenomena. Mitochondria are a frequent target of hepatotoxic drugs, and the alteration of their function has immediate effects on the ene…

Lipid PeroxidesDiclofenacDNA repairTetrazolium SaltsMitochondrionPharmacologyIn Vitro TechniquesToxicologymedicine.disease_causeGeneral Biochemistry Genetics and Molecular BiologyXenobioticsLipid peroxidationchemistry.chemical_compoundAdenosine TriphosphateDetoxificationToxicity TestsmedicineAnimalsHumansBiotransformationFormazansAnti-Inflammatory Agents Non-SteroidalGeneral MedicineGlutathioneGlutathioneRatsMedical Laboratory TechnologychemistryToxicityHepatocytesXenobioticOxidative stress
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Effects of Escherichia coli hemolysin on human monocytes. Cytocidal action and stimulation of interleukin 1 release.

1990

Abstract This study reports on the potent cytocidal and interleukin-1 releasing properties of Escherichia coli hemolysin (ECH) on human monocytes. Nanomolar concentrations of purified ECH (250-2,000 ng/ml) caused rapid and irreversible depletion of cellular ATP to levels below 20% of controls within 60 min. Subcytocidal doses (10-200 ng/ml) of ECH induced rapid release within 60-120 min of large amounts of interleukin 1 beta (IL-1 beta) from cultured monocytes. IL-1 beta release occurred in the presence of actinomycin D and cycloheximide, and was thus probably due to processing and export of intracellular IL-1 beta precursor. Incubation of toxin-producing E. coli at ratios of only 0.3-3 col…

LipopolysaccharidesCell SurvivalStimulationIn Vitro TechniquesBiologyCycloheximidemedicine.disease_causeHemolysin ProteinsMonocytesMicrobiologyHemolysin Proteinschemistry.chemical_compoundAdenosine TriphosphateEscherichia colimedicineHumansEscherichia coliCells CulturedToxinMonokinesMonocyteInterleukinDrug SynergismGeneral Medicinemedicine.anatomical_structurechemistrySecretory RateIntracellularResearch ArticleInterleukin-1Journal of Clinical Investigation
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