Search results for "hmC"
showing 7 items of 7 documents
Association of immunoglobulin GM allotypes with longevity in long-living individuals from Southern Italy
2018
Abstract Background The aim of this study was to analyse the role of GM allotypes, i.e. the hereditary antigenic determinants expressed on immunoglobulin polypeptide chains, in the attainment of longevity. The role played by immunoglobulin allotypes in the control of immune responses is well known as well as the role of an efficient immune response in longevity achievement. So, it is conceivable that particular GM allotypes may contribute to the generation of an efficient immune response that supports successful ageing, hence longevity. Methods In order to show if GM allotypes play a role in the achievement of longevity, we typed the DNA of 95 Long-living individuals (LLIs) and 96 young con…
GADD45a physically and functionally interacts with TET1
2015
AbstractDNA demethylation plays a central role during development and in adult physiology. Different mechanisms of active DNA demethylation have been established. For example, Growth Arrest and DNA Damage 45-(GADD45) and Ten-Eleven-Translocation (TET) proteins act in active DNA demethylation but their functional relationship is unresolved. Here we show that GADD45a physically interacts – and functionally cooperates with TET1 in methylcytosine (mC) processing. In reporter demethylation GADD45a requires endogenous TET1 and conversely TET1 requires GADD45a. On GADD45a target genes TET1 hyperinduces 5-hydroxymethylcytosine (hmC) in the presence of GADD45a, while 5-formyl-(fC) and 5-carboxylcyto…
Redox regulation of genome stability by effects on gene expression, epigenetic pathways and DNA damage/repair
2015
Reactive oxygen and nitrogen species (e.g. H2O2, nitric oxide) confer redox regulation of essential cellular signaling pathways such as cell differentiation, proliferation, migration and apoptosis. In addition, classical regulation of gene expression or activity, including gene transcription to RNA followed by translation to the protein level, by transcription factors (e.g. NF-κB, HIF-1α) and mRNA binding proteins (e.g. GAPDH, HuR) is subject to redox regulation. This review will give an update of recent discoveries in this field, and specifically highlight the impact of reactive oxygen and nitrogen species on DNA repair systems that contribute to genomic stability. Emphasis will be placed …
Resource or waste? A perspective of plastics degradation in soil with a focus on end-of-life options.
2018
‘Capable-of-being-shaped’ synthetic compounds are prevailing today over horn, bone, leather, wood, stone, metal, glass, or ceramic in products that were previously left to natural materials. Plastic is, in fact, economical, simple, adaptable, and waterproof. Also, it is durable and resilient to natural degradation (although microbial species capable of degrading plastics do exist). In becoming a waste, plastic accumulation adversely affects ecosystems. The majority of plastic debris pollutes waters, accumulating in oceans. And, the behaviour and the quantity of plastic, which has become waste, are rather well documented in the water, in fact. This review collects existing information on pla…
Dynamical twisted mass fermions with light quarks
2007
We present results of dynamical simulations with 2 flavours of degenerate Wilson twisted mass quarks at maximal twist in the range of pseudo scalar masses from 300 to 550 MeV. The simulations are performed at one value of the lattice spacing a \lesssim 0.1 fm. In order to have O(a) improvement and aiming at small residual cutoff effects, the theory is tuned to maximal twist by requiring the vanishing of the untwisted quark mass. Precise results for the pseudo scalar decay constant and the pseudo scalar mass are confronted with chiral perturbation theory predictions and the low energy constants F, \bar{l}_3 and \bar{l}_4 are evaluated with small statistical errors.
Role of TET3 and 5hmC in the small intestinal epithelium homeostasis
2022
The Ten-eleven translocation (TET) enzymes oxidize the DNA base 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). This DNA hydroxymethylation process generates a stable epigenetic mark, the 5hmC, that is able to regulate gene transcription. In fact, TET proteins and the 5hmC modification are relevant during embryonic development and cellular differentiation. Specifically, in the case of the small intestinal epithelium, the highest DNA hydroxymethylation levels are found at the villus, where 5hmC is preferentially deposited at genes that define mature enterocytes. However, the specific function of each of the TET enzymes in this tissue still needs to be investigated. In this context,…
Screening for Low Energy Availability in Male Athletes: Attempted Validation of LEAM-Q
2022
A questionnaire-based screening tool for male athletes at risk of low energy availability (LEA) could facilitate both research and clinical practice. The present options rely on proxies for LEA such screening tools for disordered eating, exercise dependence, or those validated in female athlete populations. in which the female-specific sections are excluded. To overcome these limitations and support progress in understanding LEA in males, centres in Australia, Norway, Denmark, and Sweden collaborated to develop a screening tool (LEAM-Q) based on clinical investigations of elite and sub-elite male athletes from multiple countries and ethnicities, and a variety of endurance and weight-sensiti…