Search results for "hydrogenase"
showing 10 items of 575 documents
Plastidial Glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase Is an Important Determinant in the Carbon and Nitrogen Metabolism of Heterotrophic Cel…
2015
This study functionally characterizes the Arabidopsis (Arabidopsis thaliana) plastidial glycolytic isoforms of glyceraldehyde-3-phosphate dehydrogenase (GAPCp) in photosynthetic and heterotrophic cells. We expressed the enzyme in gapcp double mutants (gapcp1gapcp2) under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 had no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affected Arabidopsis development by increasing the number of later…
Formation of l(-)malate by Saccharomyces cerevisiae during fermentation
1988
When grown in a synthetic medium most of the 51 strains of the genera Saccharomyces, Saccharomycodes, Zygosaccharomyces and Schizosaccharomyces investigated formed l-malate during fermentation. The quantity varied between 0.1 and 2.6 g malate per liter. Two strains of Saccharomyces cerevisiae synthesized malate at a rate of about 1.5 g/l. Malate was liberated during the growth phase and not metabolized during the stationary phase. Optimum malate formation was observed at a sugar concentration of about 20% (w/v), at pH 5 and at suboptimal nitrogen concentrations of less than 300 mg N/liter. Of the amino acids aspartate and glutamate were most favourable. If ammonium salts were used as the ni…
The Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase activity increases in response to starvation and temperature upshi…
2002
We have determined the effect of environmental factors (mild thermal upshift and starvation) on the Candida albicans cell wall-associated glyceraldehyde-3-phosphate dehydrogenase (cwGAPDH) activity. Temperature upshift (from 28 to 37 degrees C) and/ or starvation (at 28 or 37 degrees C in water) of exponentially growing yeast cells caused an increase in cwGAPDH activity (3 to 5-, and 7 to 8-fold, respectively). This increase in activity did not correlate with an increase in the amount of cwGAPDH protein present, as determined by flow cytometry, immunoelectron microscopy and Western-blotting. These results indicate that thermal upshift and starvation cause an activation of the cwGAPDH in C. …
Enzym-histochemische Studien am cervicalen Dr�senfeld des Uterus
1972
Durch enzym-histochemische Untersuchungen an Cervices soll Einblick in den Stoffwechsel des cervicalen Drusenfeldes gewonnen werden. Hierfur wurden insbesondere die Enzyme des Glucosestoffwechsels, der Fettsaureoxydation, des Pentosephosphatstoffwechsels, des Citronensaurecyclus sowie des NADH- und NADPH-Diaphorese-Systems und des Aminosaurestoffwechsels untersucht. Die einheitliche Nachweistechnik erlaubt in Form eines Art Screening-Tests eine Information uber die vorhandenen Aktivitaten der einzelnen Enzyme, wobei — abgesehen von einem Enzym des Glucosestoffwechsels, namlich der Alkoholdehydrogenase und des Aminosaurestoffwechsels, der Glutamatdehydrogenase — alle Fermente nachgewiesen we…
Nitroglycerin Hits the Nerve
2008
Organic nitrates are still widely used for the treatment of acute and chronic angina and congestive heart failure. When given acutely, their effectiveness is indisputable; in contrast, their long-term efficacy is limited because of serious side effects such as the development of tolerance and
The Structure and Metabolism of Carbohydrates
1994
Compared with the variety of carbohydrates in plants, relatively few sugars or sugar derivatives are regularly found in animals either free or as components of more complex compounds. However, it is possible that sugars originating from plants in the diet are transiently retained in animals and distort the normal sugar spectrum. Approximately a dozen sugars and sugar derivatives are regularly found in animals: the pentoses d-ribose and 2-deoxy-d-ribose; the hexoses d-glucose, d-galactose, d-mannose, d-fructose and l-fucose; the uronic acids d-glucuronic acid and l-iduronic acid; and the hexosamines d-glucosamine and d-galactosamine. In addition, d-erythrose, d-ribulose, d-xylulose and d-sed…
Interaction of the mitochondrial membrane D-3-hydroxybutyrate dehydrogenase with fluorescent phospholipids
1993
Norbert Latruffe l ,I, Boubker Nasser ‘, Claude Morpain 3, Jiirgen Zirkel 4, Michael Seiter 4, Bernard Laude 3 and Wolfgang Trommer 4 ’ Laboratoire de Biobgie Mol&laire et Cellulaire, Universite’ de Bourgogne, Fact& des Sciences Mirande, BP 138, 21004 D@on Cedex (France) 2 Laboratoire de Biochimie &A CNRS 531) and 3 Laboratoire de Chimie Organique, Universite’ de Franche-Comte 25030 Besaqon Cedex (France) 4 Fachbereich Chemie, Universitiit Kaiserslautem, D 6750 Kaiserslautem (Germany)
ChemInform Abstract: Synthesis and Pharmacological Evaluation of 1-Methyl-5- [substituted-4(3H)-oxo-1,2,3-benzotriazin-3-yl]-1H-pyrazole-4-acetic Aci…
2010
Abstract Several new 1-methyl-5-[substituted-4-oxo-1,2,3-benzotriazin-3-yl]-1H-pyrazole-4-acetic acids and their ethyl ester derivatives were prepared. The compounds were tested for analgesic and antiinflammatory activities, acute toxicity, ulcerogenic effect, and as in vitro inhibitors of 3α-hydroxysteroid dehydrogenase (3α-HSD), since it is claimed that the inhibition of such an enzyme predicts in vivo antiinflammatory activity. Some compounds were more active than phenylbutazone in the phenylbenzoquinone and acetic acid peritonitis tests, and equiactive to the same drug in the carrageenin paw edema test. All the compounds inhibited the 3α-HSD, but no correlation was observed with the paw…
Messung der gesamtaktivität von glutamat-oxalacetat-transaminase in leberhomogenaten
1971
Abstract A method is described, suitable for routine measurements of the total glutamicoxaloacetic transaminase activity in a large number of liver samples by treatment of homogenates with the detergent Triton X-100. Comparison with other methods and with results of other investigators shows that this method is superior. Neither sonic treatment nor freezing and thawing leads to a complete liberation of the mitochondrial transaminase activity. A number of other enzyme activities can be assayed in Triton-treated homogenates, such as glutamic-pyruvic transaminase, glutamate dehydrogenase, NADP-isocitric dehydrogenase, lactate dehydrogenase, and malate dehy drogenase, β-Hydroxybutyrate dehydrog…
Interactions of the mitochondrial membrane rat liver d-3-hydroxybutyrate dehydrogenase with glass beads during adsorption chromatography
1991
D-3-Hydroxybutyrate dehydrogenase (BDH) is an NAD(+)-dependent dehydrogenase of the mitochondrial inner membrane involved in the energetic balance between the liver and peripheral organs in mammals. It allows the conversion of ketone bodies (acetoacetate and D-3-hydroxybutyrate) and it is one of the best documented lipid-requiring enzymes with a dependence on lecithins. After release of proteins from the membrane by phospholipase A2 treatment of salt-treated mitochondria, the rat liver enzyme is absorbed on controlled-pore glass beads. After batch washing, the enzyme, devoid of lipids (apoBDH), is specifically eluted at pH 8.05-8.15 with a 0.1 M Tris-1 M LiBr buffer under reducing condition…