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showing 10 items of 8115 documents

Real-time polarimetric biosensing using macroporous alumina membranes

2013

We report the first demonstration of real-time biosensing in free standing macroporous alumina membranes. The membranes with their 200 nm diameter pores are ideal candidates for biosensing applications where fast response times for small sample volumes are needed as they allow analytes to flow through the pores close to the bioreceptors immobilized on the pores walls. A bulk refractive index sensitivity of 5.2x10 -6 refractive index units was obtained from signal responses to different concentrations of NaCl solutions flowing through the pores. Finally, after functionalizing the alumina pore surfaces with an epoxysilane and then spotting it with β-Lactoglobulin protein, the interactions bet…

AnalyteMaterials scienceNacl solutionsAlumina membranesAnalytical chemistryfood and beveragesSmall sampleRabbit (nuclear engineering)02 engineering and technology021001 nanoscience & nanotechnology01 natural sciences010309 opticsMembrane0103 physical sciences0210 nano-technologyBiosensorRefractive indexSPIE Proceedings
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Characterization of porous alumina membranes for efficient, real-time, flow through biosensing

2015

Abstract Nanofluidic sensing devices promise high performance by overcoming issues of mass transport of analyte molecules to the sensing surface, whilst micro-porous membranes promise high sensitivity due to a large surface for their capture. Anodic alumina (AAO) filter membranes allow the flow through of samples, and could be used as a convenient and readily available fluidic platform for the targeted delivering of analytes to bioreceptors immobilized on the pore walls. The relatively small pore dimensions, compared to fluidic diffusion lengths, promise highly efficient capture of analytes from the whole sample volume, enabling relatively fast sensing response times and the use of small sa…

AnalyteMaterials sciencegenetic structuresQuantum dotsDiffusionFiltration and SeparationNanotechnologyPorous aluminaPore size distributionBiochemistryCharacterization (materials science)AnodeMembraneGeneral Materials ScienceFluidicsPhysical and Theoretical ChemistryPorosityBiosensorOptical biosensing and sensorsProtein physisorption
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Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection

2019

In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized with capture DNA sequences complementary to the analyte (target) DNA. Upon incubation with the target sequence, it will bind on the respectively complementary functionalized particle spot. This binding changes the local refractive index, which is detected spectroscopically as the resulting changes of the localized surface plasmon resonance (LSPR) peak wavelength. In order to increase the signal, a small gold nanoparticle label is introduced. The binding ca…

AnalyteMaterials scienceta221Molecular bindingMetal NanoparticlesNanoparticleBioengineering02 engineering and technologybiosensorit01 natural sciencesSensor arrayLimit of DetectionNanosensorplasmonic arrayNanotechnologyLSPR sensingSurface plasmon resonanceDNA FungalInstrumentationPlasmonCandidaDNA detectionFluid Flow and Transfer ProcessesPlasmonic nanoparticlesBase Sequenceta114business.industryProcess Chemistry and Technology010401 analytical chemistryta1182Fourier-transform-imaging spectroscopynanobiotekniikkaDNASurface Plasmon Resonance021001 nanoscience & nanotechnologyplasmonic nanoparticles0104 chemical sciencesAspergillusOptoelectronicsnanohiukkasetGoldDNA Probes0210 nano-technologybusinessACS Sensors
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Noninvasive sampling of phenylalanine by reverse iontophoresis.

1999

While iontophoresis is typically associated with drug delivery across the skin, the symmetry of the technique permits its application to the essentially noninvasive withdrawal of biologically important analytes from the subcutaneous space to the body's surface. The identification of other substances which can be monitored by this procedure, and to its optimization and development as a more general clinical chemistry tool, is a long-term objective. In this paper, we describe a preliminary in vitro investigation into the feasibility of extracting and analyzing the amino acid, phenylalanine, with the ultimate aim to develop a diagnostic test for phenylketonuria, a potentially fatal metabolic d…

AnalytePathologymedicine.medical_specialtyNoninvasive samplingIontophoresisbusiness.industryPhenylalaninePharmaceutical SciencePhenylalanineHydrogen-Ion ConcentrationIontophoresisSodium ChlorideIn vivoDrug deliverymedicineHumansMetabolic diseasebusinessBiosensorEdetic AcidBiomedical engineeringJournal of controlled release : official journal of the Controlled Release Society
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"Un puente sobre el abismo", de Higinio Noja Ruiz (1932) : cultura libertaria y literatura pacifista de entreguerras

2021

El escritor, ensayista y militante anarquista Higinio Noja Ruiz publicó en 1932 la novela Un puente sobre el abismo en la Editorial Estudios de Valencia. En ella Noja abordó el tema de las guerras y la lucha contra ellas a través de su protagonista, Guillermo Arjona, un joven burgués mallorquín muy influido por su lectura de las novelas sobre la Primera Guerra Mundial y casi obsesionado con esta temática. Se analiza aquí en primer lugar la figura de Noja Ruiz (1994-1972), prolífico novelista social a la vez que ensayista y pensador libertario de las décadas de 1920 y 1930. La trayectoria de Noja es inseparable de la de la revista cultural y editorial libertaria valenciana Estudios (1928-193…

AnarquismoHistoryPacifismeEstudios. Revista eclécticapacifismoAnarchismAnti-war literatureHiginio Noja RuizPacifismModern history 1453-anarquismoD204-475Estudios. Revista EclécticaPrimera Guerra MundialPrimera guerra mundialliteratura antibélicaLiteratura antibèl·licaLiteratura antibélicaAnarquismeWorld war iPacifismo
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Allometries of maximum growth rate versus body mass at maximum growth indicate that non-avian dinosaurs had growth rates typical of fast growing ecto…

2014

We tested if growth rates of recent taxa are unequivocally separated between endotherms and ectotherms, and compared these to dinosaurian growth rates. We therefore performed linear regression analyses on the log-transformed maximum growth rate against log-transformed body mass at maximum growth for extant altricial birds, precocial birds, eutherians, marsupials, reptiles, fishes and dinosaurs. Regression models of precocial birds (and fishes) strongly differed from Case's study (1978), which is often used to compare dinosaurian growth rates to those of extant vertebrates. For all taxonomic groups, the slope of 0.75 expected from the Metabolic Theory of Ecology was statistically supported. …

Anatomy and PhysiologyMetabolic theory of ecologyVertebrate Paleontologylcsh:MedicineBiostatisticsBody Mass IndexDinosaursBirdsbiology.animalAnimalsGrowth rateStatistical Methodslcsh:ScienceBiologyEvolutionary BiologyMultidisciplinarybiologyEcologyEcologyFossilsApplied Mathematicslcsh:RStatisticsFishesVertebrateReptilesPaleontologyThermoregulationBiological EvolutionAltricialTaxonMarsupialiaEctothermEarth Scienceslcsh:QPrecocialPaleobiologyPhysiological ProcessesEnergy MetabolismMathematicsResearch ArticleDevelopmental BiologyPloS one
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Microsporidia and Its Relation to Crohn's Disease. A Retrospective Study

2013

Background: The cause of Crohn's Disease (CD) remains unknown. Recently a decrease in the global lymphocyte population in the peripheral blood of CD patients has been reported. This decrease was more evident in gamma delta T lymphocytes, especially gamma delta CD8+T subsets. Furthermore, a decrease of IL-7 was also observed in these patients. We propose the hypothesis that microsporidia, an obligate intracellular opportunistic parasite recently related to fungi, in CD patients can take advantage of the lymphocytes and IL-7 deficits to proliferate and to contribute to the pathophysiology of this disease. Methods and Findings: In this case-control study, serum samples were collected from 36 C…

Anatomy and PhysiologyNon-Clinical MedicineLymphocytePopulationlcsh:MedicineDiseaseGastroenterology and HepatologyBiologyReal-Time Polymerase Chain ReactionBiochemistryAntibodiesCrohn DiseaseT-Lymphocyte SubsetsImmune PhysiologyMicrosporidiosismedicineParasitic DiseasesCytotoxic T cellHumanslcsh:ScienceeducationBiologyRetrospective Studieseducation.field_of_studyCrohn's diseaseMultidisciplinaryHealth Care Policylcsh:RInflammatory Bowel DiseaseCase-control studyFungal DiseasesHealth Risk AnalysisEncephalitozoonImmunoglobulin Emedicine.diseaseVirologyPathophysiologymedicine.anatomical_structureInfectious DiseasesCase-Control StudiesImmunologyBlood ChemistryMicrosporidiaMedicinelcsh:QCD8Research Article
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Data from: Parallel diversifications of Cremastosperma and Mosannona (Annonaceae), tropical rainforest trees tracking Neogene upheaval of South Ameri…

2017

Much of the immense present day biological diversity of Neotropical rainforests originated from the Miocene onwards, a period of geological and ecological upheaval in South America. We assess the impact of the Andean orogeny, drainage of lake Pebas, and closure of the Panama Isthmus on two clades of tropical trees (Cremastosperma, c. 31 spp.; and Mosannona, c. 14 spp.; both Annonaceae). Phylogenetic inference revealed similar patterns of geographically restricted clades and molecular dating showed diversifications in the different areas occurred in parallel, with timing consistent with Andean vicariance and Central American geodispersal. Ecological niche modelling approaches show phylogenet…

Andean orogenyAnnonaceaePE&RCLife sciencesMalmeoideaeBiosystematiekmedicine and health careMosannonaMedicineBiosystematicsCremastospermaniche modellingNeogeneEPSLaboratory of Nematologymolecular datingLaboratorium voor NematologieNiche ModellingPebas system
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Chiral Monofluorobenzyl Carbanions: Synthesis of Enantiopure β‐Fluorinated β‐Phenylethylamines

2011

The preparation of a stabilized monofluorobenzyl carbanion by means of a remote homochiral sulfinyl group and its completely stereoselective reactions with N-p-tolylsulfinylimines are described. The use of these reactions followed by the simultaneous removal of both chiral auxiliaries with tBuLi, which occurs without epimerization at the benzylic position, provides the quickest entry to enantiomerically pure β-fluorinated β-phenylethylamines.

AnionsHalogenationStereochemistryOrganic ChemistryMolecular ConformationEnantioselective synthesisStereoisomerismGeneral ChemistryCatalysischemistry.chemical_compoundEnantiopure drugchemistryBenzyl CompoundsPhenethylaminesIonic liquidStereoselectivityEpimerIminesCarbanionChemistry – A European Journal
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A highly enantioselective abiotic receptor for malate dianion in aqueous solution

2006

The highly enantioselective molecular recognition of the malate dianion by a synthetic receptor in aqueous solution has been studied by potentiometric titrations, mass spectrometry (ESI-MS), diffusion measurements (PGSE NMR) and molecular modeling. Garcia-España Monsonis, Enrique, Enrique.Garcia-Es@uv.es

AnionsModels MolecularMalate dianionSpectrometry Mass Electrospray IonizationMagnetic Resonance SpectroscopyMolecular modelUNESCO::QUÍMICADiffusionPotentiometric titrationMalatesMolecular modelingAbiotic receptorMass spectrometry:QUÍMICA [UNESCO]CatalysisSubstrate SpecificityMolecular recognitionComputational chemistryMaterials ChemistryOrganic chemistryAqueous solutionReceptorAbiotic componentEnantioselectiveAqueous solutionMolecular StructureChemistrySpectrometryMeasurementsMetals and AlloysEnantioselective synthesisWaterHydrogen BondingStereoisomerismGeneral ChemistryGeneral MedicineUNESCO::QUÍMICA::Química macromolecularCombinatorial chemistrySurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsSolutionsCeramics and CompositesPotentiometryEnantioselective ; Abiotic receptor ; Malate dianion ; Aqueous solution ; Spectrometry ; Measurements ; Molecular modeling:QUÍMICA::Química macromolecular [UNESCO]
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