Search results for "ice"

showing 10 items of 26338 documents

Cohen Syndrome-Associated Cataract Is Explained by VPS13B Functions in Lens Homeostasis and Is Modified by Additional Genetic Factors

2020

International audience; Purpose: Cohen syndrome (CS) is a rare genetic disorder caused by variants of the VPS13B gene. CS patients are affected with a severe form of retinal dystrophy, and in several cases cataracts also develop. The purpose of this study was to investigate the mechanisms and risk factors for cataract in CS, as well as to report on cataract surgeries in CS patients.Methods: To understand how VPS13B is associated with visual impairments in CS, we generated the Vps13b∆Ex3/∆Ex3 mouse model. Mice from 1 to 3 months of age were followed by ophthalmoscopy and slit-lamp examinations. Phenotypes were investigated by histology, immunohistochemistry, and western blot. Literature anal…

0301 basic medicinegenetic structuresDevelopmental DisabilitiesVesicular Transport Proteins030105 genetics & hereditysurgerygenetic backgroundchemistry.chemical_compoundLensMyopiaHomeostasisMice KnockoutCohen syndrome[SDV.MHEP] Life Sciences [q-bio]/Human health and pathologymedicine.diagnostic_testRetinal DegenerationGenetic disorderinflamma- tionVPS13BcataractKnockout mouseMicrocephalyMuscle Hypotoniamedicine.medical_specialtymouse modelBlotting WesternRetinitisFingersOphthalmoscopy03 medical and health sciencesCataractsIntellectual DisabilityOphthalmologyVPS13BLens CrystallinemedicineAnimalsObesityCohen syndromebusiness.industryfibrosisRetinalgenetic modifiersmedicine.diseaseeye diseasesMice Inbred C57BLDisease Models Animalophthalmology030104 developmental biologyGene Expression RegulationchemistryinflammationRNAsense organsbusiness[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyInvestigative Ophthalmology & Visual Science
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Frequent Ultrafine Particle Formation and Growth in the Canadian Arctic Marine Environment

2017

Abstract. The source strength and capability of aerosol particles in the Arctic to act as cloud condensation nuclei have important implications for understanding the indirect aerosol-cloud effect within the polar climate system. It has been shown in several Arctic regions that ultrafine particle (UFP) formation and growth is a key contributor to aerosol number concentrations during the summer. This study uses aerosol number size distribution measurements from ship-board measurement expeditions aboard the research icebreaker CCGS Amundsen in the summers of 2014 and 2016 throughout the Canadian Arctic to gain a deeper understanding of the drivers of UFP formation and growth within this marine…

0301 basic medicinegeographygeography.geographical_feature_category010504 meteorology & atmospheric sciencesAtmospheric sciences01 natural sciencesSink (geography)LatitudeAerosol03 medical and health sciences030104 developmental biologyArctic13. Climate actionUltrafine particleSea iceCloud condensation nucleiEnvironmental science14. Life underwaterPolar climate0105 earth and related environmental sciences
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Immunization Status against Measles of Health-Care Workers Operating at Three Sicilian University Hospitals: An Observational Study

2019

Measles is a highly contagious vaccine-preventable disease (VPD) that also commonly affects health-care workers (HCWs). Measles immunization of HCWs was strongly recommended by international health authorities, in order to limit the spreading of the illness to susceptible patients and colleagues. An observational study, evaluating the immunization and vaccination status against measles of HCWs working at three Sicilian university hospitals, was conducted. All subjects not completely immune (vaccinated with only one dose in their lifetime), not immune (not vaccinated or not naturally immunized), and with an unknown immunization status were considered not immunized. Among HCWs operating in th…

0301 basic medicinehealth care facilities manpower and servicesanimal diseases030106 microbiologyImmunologyeducationlcsh:Medicinechemical and pharmacologic phenomenaDiseaseSettore MED/42 - Igiene Generale E ApplicataMeaslesArticle03 medical and health sciences0302 clinical medicineEnvironmental healthDrug DiscoveryHealth careHealth-care personnel Vaccine hesitancy Vaccine-preventable diseasemedicinePharmacology (medical)vaccine-preventable disease030212 general & internal medicinePharmacologybusiness.industrylcsh:RInternational healthhealth-care personnelbiochemical phenomena metabolism and nutritionmedicine.diseaseUniversity hospitalVaccinationInfectious DiseasesImmunizationHealth-care personnel; Vaccine hesitancy; Vaccine-preventable diseasebacteriavaccine hesitancyObservational studybusinessVaccines
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Real life experiences in HCV management in 2018

2019

Introduction: Hepatitis C virus (HCV) infection is a major cause of chronic liver disease, with approximately 71 million chronically infected individuals worldwide. Treatment of chronic hepatitis C has considerably improved in the last few years thanks to the introduction of direct-acting antivirals able to achieve sustained virological response in more than 95% of patients. Successful anti-HCV treatment can halt liver disease progression and solve the HCV-related extra-hepatic manifestations, eventually reducing liver-related and overall mortality. Areas covered: With the aim to respond to unmet needs in patient’s identification, universal access to antiviral therapy and treatment optimiza…

0301 basic medicinehepatitis C virusSofosbuvirSustained Virologic ResponseAntiviral therapyAntiviral therapy; chronic liver disease; DAAs; HCV; hepatitis C virus; Microbiology; Microbiology (medical); Infectious Diseases; Virologymedicine.disease_causeChronic liver diseaseHealth Services Accessibility0302 clinical medicinedirect acting antiviralshepatitis C viruMass Screening030212 general & internal medicineChronicComputingMilieux_MISCELLANEOUSHepatitis CHepatitis BHepatitis CPibrentasvirAntiviral therapy; chronic liver disease; DAAs; HCV; hepatitis C virus; Antiviral Agents; Disease Progression; Health Services Accessibility; Hepatitis C Chronic; Humans; Italy; Mass Screening; Sustained Virologic ResponseInfectious DiseasesItalyHCVDisease ProgressionAntiviral therapy; chronic liver disease; DAAs; HCV; hepatitis C virus; Antiviral Agents; Disease Progression; Health Services Accessibility; Hepatitis C; Chronic; Humans; Italy; Mass Screening; Sustained Virologic Responsemedicine.drugHumanMicrobiology (medical)Settore MED/17 - Malattie InfettiveHepatitis C virus030106 microbiologyInfectious DiseaseAntiviral AgentsMicrobiology03 medical and health sciencesVirologymedicineHumansAntiviral therapy; DAAs; HCV; chronic liver disease; direct acting antivirals; hepatitis C virusMass screeningDAAHepatitis B virusAntiviral Agentbusiness.industrychronic liver diseaseDAAsHepatitis C Chronicmedicine.diseaseVirologybusiness
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Dlk1 dosage regulates hippocampal neurogenesis and cognition

2021

Significance Generation of new neurons occurs normally in the adult brain in two locations: the subventricular zone (SVZ) in the walls of the lateral ventricles and the subgranular zone (SGZ) in the dentate gyrus (DG) of the hippocampus. Neurogenesis in the adult hippocampus has been implicated in cognitive functions such as learning, memory, and recovery of stress response. Imprinted genes are highly prevalent in the brain and have adult and developmental important functions. Genetic deletion of the imprinted gene Dlk1 from either parental allele shows that DLK1 is a key mediator of quiescence in adult hippocampal NSCs. Additionally, Dlk1 is exquisitely dosage sensitive in the brain with p…

0301 basic medicinehippocampusHippocampusgene dosageBiologySubgranular zone03 medical and health sciencesMice0302 clinical medicineCognitionNeuroplasticitymedicineAnimalsEpigeneticsImprinting (psychology)AllelesMultidisciplinarybehaviorDentate gyrusNeurogenesisCalcium-Binding Proteinsneurogenesis genomic imprinting behavior gene dosage hippocampus424Biological Sciencesgenomic imprintingneurogenesis030104 developmental biologymedicine.anatomical_structurenervous systemGenomic imprintingNeuroscience030217 neurology & neurosurgeryNeuroscience
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Multicellular Interactions in 3D Engineered Myocardial Tissue

2018

Cardiovascular disease is a leading cause of death in the US and many countries worldwide. Current cell-based clinical trials to restore cardiomyocyte (CM) health by local delivery of cells have shown only moderate benefit in improving cardiac pumping capacity. CMs have highly organized physiological structure and interact dynamically with non-CM populations, including endothelial cells and fibroblasts. Within engineered myocardial tissue, non-CM populations play an important role in CM survival and function, in part by secreting paracrine factors and cell-cell interactions. In this review, we will summarize the progress of engineering myocardial tissue with pre-formed physiological multice…

0301 basic medicinelcsh:Diseases of the circulatory (Cardiovascular) systemMini Reviewcardiomyocyte02 engineering and technologyDiseaseCardiovascular MedicineBiologyengineered myocardiumfibroblast03 medical and health sciencesParacrine signallingcardiovascular tissue engineeringMyocardial tissueTranslation (biology)021001 nanoscience & nanotechnologyco-culture3. Good healthCell biologystem cellEndothelial stem cellMulticellular organism030104 developmental biologylcsh:RC666-701endothelial cellStem cell0210 nano-technologyCardiology and Cardiovascular MedicineFunction (biology)Frontiers in Cardiovascular Medicine
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RNase H2 Loss in Murine Astrocytes Results in Cellular Defects Reminiscent of Nucleic Acid-Mediated Autoinflammation

2018

Aicardi-Goutières syndrome (AGS) is a rare early onset childhood encephalopathy caused by persistent neuroinflammation of autoimmune origin. AGS is a genetic disorder and >50% of affected individuals bear hypomorphic mutations in ribonuclease H2 (RNase H2). All available RNase H2 mouse models so far fail to mimic the prominent CNS involvement seen in AGS. To establish a mouse model recapitulating the human disease, we deleted RNase H2 specifically in the brain, the most severely affected organ in AGS. Although RNase H2δGFAPmice lacked the nuclease in astrocytes and a majority of neurons, no disease signs were apparent in these animals. We additionally confirmed these results…

0301 basic medicinelcsh:Immunologic diseases. AllergyMaleEncephalomyelitis Autoimmune ExperimentalAicardi–Goutières syndromeRNase PDNA damageImmunologyRibonuclease HFluorescent Antibody TechniqueAicardi-goutières Syndrome ; Cellular Senescence ; Dna Damage ; Interferon Signature ; Rnase H2BiologyNervous System MalformationsAutoimmune Diseases03 medical and health sciencesMiceAutoimmune Diseases of the Nervous SystemNucleic AcidsmedicineImmunology and Allergycellular senescenceAnimalsRibonucleaseNeuroinflammationCells CulturedOriginal ResearchInflammationMice KnockoutInnate immune systemBrainmedicine.diseaseMolecular biologyImmunohistochemistryDisease Models Animal030104 developmental biologymedicine.anatomical_structurePhenotypeinterferon signatureAstrocytesKnockout mousebiology.proteinAicardi–Goutières syndromeDNA damageFemalelcsh:RC581-607RNase H2BiomarkersAstrocyteFrontiers in Immunology
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Gamma-Delta CAR-T Cells Show CAR-Directed and Independent Activity Against Leukemia

2020

Autologous T cells engineered to express a chimeric antigen receptor (CAR) against the CD19 antigen are in the frontline of contemporary hemato-oncology therapies, leading to high remission rates in B-cell malignancies. Although effective, major obstacles involve the complex and costly individualized manufacturing process, and CD19 target antigen loss or modulation leading to resistant and relapse following CAR therapy. A potential solution for these limitations is the use of donor-derived γδT cells as a CAR backbone. γδT cells lack allogenecity and are safely used in haploidentical transplants. Moreover, γδT cells are known to mediate natural anti-tumor responses. Here, we describe a 14-da…

0301 basic medicinelcsh:Immunologic diseases. Allergymedicine.medical_treatmentImmunologyCell Culture TechniquesPriming (immunology)Mice SCIDImmunotherapy AdoptiveCD1903 medical and health sciencesMice0302 clinical medicineAntigenMice Inbred NODTransduction GeneticmedicineAnimalsHumansImmunology and Allergyimmuno oncologyB cell malignanciesOriginal ResearchLeukemiaReceptors Chimeric Antigenbiologychimeric antigen receptorChemistrygamma-delta T cellsReceptors Antigen T-Cell gamma-deltamedicine.diseaseXenograft Model Antitumor AssaysChimeric antigen receptorLeukemia030104 developmental biologyCytokinemedicine.anatomical_structureCell cultureCancer researchbiology.proteinBone marrowlcsh:RC581-607Genetic Engineering030215 immunologyFrontiers in Immunology
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The Unfolded Protein Response Plays a Predominant Homeostatic Role in Response to Mitochondrial Stress in Pancreatic Stellate Cells.

2016

Activated pancreatic stellate cells (PaSC) are key participants in the stroma of pancreatic cancer, secreting extracellular matrix proteins and inflammatory mediators. Tumors are poorly vascularized, creating metabolic stress conditions in cancer and stromal cells that necessitate adaptive homeostatic cellular programs. Activation of autophagy and the endoplasmic reticulum unfolded protein response (UPR) have been described in hepatic stellate cells, but the role of these processes in PaSC responses to metabolic stress is unknown. We reported that the PI3K/mTOR pathway, which AMPK can regulate through multiple inputs, modulates PaSC activation and fibrogenic potential. Here, using primary a…

0301 basic medicinelcsh:MedicineApoptosisMitochondrionAMP-Activated Protein KinasesEndoplasmic ReticulumBiochemistrychemistry.chemical_compoundMiceeIF-2 KinasePhosphatidylinositol 3-Kinases0302 clinical medicineFluorescence MicroscopyCell SignalingTumor Microenvironment2.1 Biological and endogenous factorsSmall interfering RNAsAetiologylcsh:ScienceEnergy-Producing OrganellesCancerMice KnockoutMicroscopyMultidisciplinarySecretory PathwayCell DeathTOR Serine-Threonine KinasesLight MicroscopySignaling CascadesCell biologyMitochondriaNeoplasm ProteinsUp-RegulationNucleic acidsCell Processes030220 oncology & carcinogenesisCellular Structures and OrganellesResearch ArticleSignal TransductionProgrammed cell deathCell PhysiologyGeneral Science & TechnologyAutophagic Cell DeathKnockoutBiologyBioenergeticsResearch and Analysis MethodsStress Signaling Cascade03 medical and health sciencesGeneticsAutophagyAnimalsNon-coding RNAPancreasPI3K/AKT/mTOR pathwaylcsh:RAutophagyAMPKBiology and Life SciencesCell BiologyCell MetabolismGene regulationPancreatic NeoplasmsEnzyme Activation030104 developmental biologychemistryHepatic stellate cellUnfolded protein responseUnfolded Protein ResponseRNAlcsh:QGene expressionInterleukin-4Digestive DiseasesRottlerinTranscription Factor CHOP
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8.2 ka event North Sea hydrography determined by bivalve shell stable isotope geochemistry.

2019

AbstractThe abrupt 8.2 ka cold event has been widely described from Greenland and North Atlantic records. However, its expression in shelf seas is poorly documented, and the temporal resolution of most marine records is inadequate to precisely determine the chronology of major events. A robust hydrographical reconstruction can provide an insight on climatic reaction times to perturbations to the Atlantic Meridional Overturning Circulation. Here we present an annually-resolved temperature and water column stratification reconstruction based on stable isotope geochemistry of Arctica islandica shells from the Fladen Ground (northern North Sea) temporally coherent with Greenland ice core record…

0301 basic medicinelcsh:MedicineStratification (water)PalaeoclimateArticle03 medical and health sciences0302 clinical medicineIce corePalaeoceanography14. Life underwaterlcsh:ScienceBivalve shellNorth seaArctica islandicaMultidisciplinarybiologylcsh:Rbiology.organism_classification030104 developmental biologyOceanography13. Climate actionIsotope geochemistrylcsh:QHydrography030217 neurology & neurosurgeryGeologyChronologyScientific reports
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