Search results for "illumina"

showing 10 items of 153 documents

Seagrass ecosystem response to long-term high CO2 in a Mediterranean volcanic vent

2014

We examined the long-term effect of naturally acidified water on a Cymodocea nodosa meadow growing at a shallow volcanic CO2 vent in Vulcano Island (Italy). Seagrass and adjacent unvegetated habitats growing at a low pH station (pH = 7.65 ± 0.02) were compared with corresponding habitats at a control station (pH = 8.01 ± 0.01). Density and biomass showed a clear decreasing trend at the low pH station and the below- to above-ground biomass ratio was more than 10 times lower compared to the control. C content and delta 13C of leaves and epiphytes were significantly lower at the low pH station. Photosynthetic activity of C. nodosa was stimulated by low pH as seen by the significant increase in…

Ocean Acidification International Coordination Centre (OA-ICC)TemperateSalinityChlorophyll ainorganicAlkalinityLight saturation point standard errorPhotosynthetic quantum efficiencyMediterranean Sea Acidification in a Changing Climate MedSeATemperature waterCarbon inorganic dissolvedCalculated using seacarb after Nisumaa et al 2010IrradianceRespiration rate carbonAragonite saturation stateBiomassAlkalinity totalIrradiance standard errortotalCO2 ventCymodocea nodosapHRespirationEpiphytes loadMaximum photochemical quantum yield of photosystem II standard errorNet community production of carbonTemperaturePartial pressure of carbon dioxide (water) at sea surface temperature (wet air) standard errordissolvedRespiration rate carbon standard errorCarbonate ionMaximum photochemical quantum yield of photosystem IIPartial pressure of carbon dioxide (water) at sea surface temperature (wet air)Net community production of carbon standard errorIlluminance standard errorSoft bottom communitystandard errorCarbon inorganic dissolved standard errorRespiration rateElectron transport rate standard errorEarth System Researchδ13CPhotosynthetic quantum efficiency standard errorField observationChlorophyll a standard errorGross primary production of carbonBiomass standard errorCalcium carbonatePotentiometric titrationCalcite saturation stateShoot densityPotentiometricwaterIlluminanceOxygen standard errorBenthosAlkalinity total standard errorMediterranean Sea Acidification in a Changing Climate (MedSeA)Electron transport rateLight saturation pointOcean Acidification International Coordination Centre OA ICCMediterranean SeaGross primary production of carbon standard errorBicarbonate ionSoft-bottom communityδ13C standard errorTemperature water standard errorCalculated using seacarb after Nisumaa et al. (2010)Primary production PhotosynthesisSpeciespH standard errorCarbonate system computation flagloadPrimary production/PhotosynthesisFugacity of carbon dioxide (water) at sea surface temperature (wet air)CarbonBiomass/Abundance/Elemental compositionTreatmentEpiphytes load standard errorOxygenPartial pressure of carbon dioxide water at sea surface temperature wet airEpiphytes loadCarbon dioxideCarbon standard errorEntire communityFugacity of carbon dioxide water at sea surface temperature wet airGroupBiomass Abundance Elemental compositionCoast and continental shelfEpiphytesShoot density standard errorCalcium carbonate standard error
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Improvement of two-dimensional structured illumination microscopy with an incoherent illumination pattern of tunable frequency.

2018

In two-dimensional structured illumination microscopy (2D-SIM), high-resolution images with optimal optical sectioning (OS) cannot be obtained simultaneously. This tradeoff can be overcome by using a tunable-frequency 2D-SIM system and a proper reconstruction method. The goal of this work is twofold. First, we present a computational approach to reconstruct optical-sectioned images with super-resolution enhancement (OS-SR) by using a tunable SIM system. Second, we propose an incoherent tunable-frequency 2D-SIM system based on a Fresnel biprism implementation. Integration of the proposed computational method with this tunable structured illumination (SI) system results in a new 2D-SIM system…

Optical sectioningComputer scienceImage qualitymedia_common.quotation_subjectMultispectral imageStructured illumination microscopy02 engineering and technologyLateral resolution01 natural scienceslaw.invention010309 opticsComputational photographyOpticslaw0103 physical sciencesContrast (vision)Electrical and Electronic EngineeringEngineering (miscellaneous)media_commonbusiness.industry021001 nanoscience & nanotechnologySuperresolutionAtomic and Molecular Physics and OpticsLens (optics)Noise (video)0210 nano-technologybusinessApplied optics
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Far-Field Optical Control of a Movable Subdiffraction Light Grid

2012

International audience; We demonstrate experimentally a subdiffraction light pattern, with a period down to 150 nm, at the surface of an optimized silicon nanostructured thin film. We show, using near-field and far-field characterization, that this subdiffraction pattern can be translated and rotated just by changing the illumination angle. The movable high frequency light pattern paves the way for subdiffraction resolution surface imaging microscopy without scanning near-field probes.

Optics and PhotonicsSiliconMaterials scienceLightSiliconPHASEPhase (waves)General Physics and Astronomychemistry.chemical_elementNear and far field02 engineering and technology01 natural sciences010309 opticsOptics0103 physical sciencesMicroscopyNanotechnologyScattering RadiationThin filmbusiness.industryResolution (electron density)Models Theoretical021001 nanoscience & nanotechnologyNanostructuresCharacterization (materials science)Microscopy FluorescenceIllumination anglechemistryRESOLUTION[SPI.OPTI]Engineering Sciences [physics]/Optics / PhotonicOptoelectronics[ SPI.OPTI ] Engineering Sciences [physics]/Optics / Photonic0210 nano-technologybusiness
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Optimal design of incoherent tunable-frequency structured illumination microscope scheme

2018

Structured illumination microscopy (SIM) improves resolution and optical sectioning capability compared to conventional widefield techniques. The main idea of this method is the illumination of the sample with a structured pattern of fixed spatial modulation frequency. Previously, a Fresnel biprism has been implemented in a structured illumination (SI) device providing tunable-frequency sinusoidal patterns. However, the use of this SI system introduces a tradeoff between the visibility and field of view of the illumination fringes. In this contribution, we analyze theoretically this tradeoff and propose the optimal design for the Fresnel biprism-based SIM system.

Optimal designScheme (programming language)MicroscopeOptical sectioningbusiness.industryComputer scienceResolution (electron density)Visibility (geometry)ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISIONStructured illumination microscopyField of viewSample (graphics)law.inventionOpticslawbusinesscomputercomputer.programming_language2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018)
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Modeling of light pipes for the optimal disposition in buildings

2019

A light pipe is an excellent solution to transport and distribute daylight into environments without or with little lighting, guaranteeing comfort inside the rooms. As stated in the literature, the evaluation of the performances of light pipes presents numerous complexities, making the work very difficult for technicians and designers. This study is aimed to present a methodology that is able to identify the potential of light pipes using indices such as daylight autonomy (DA), continuous daylight autonomy (DAc), and useful daylight illuminance (UDI). This paper presents an analysis of daylight obtained by several configurations of simple models of light pipes installed into a 5 &times

OptimizationControl and Optimizationdaylighting comfort020209 energy0211 other engineering and technologiesEnergy Engineering and Power Technology02 engineering and technologyDaylight indexdaylight autonomyLight pipelight pipe021105 building & constructionDaylighting comfort0202 electrical engineering electronic engineering information engineeringDaylightDAYSIMElectrical and Electronic EngineeringEngineering (miscellaneous)Daylight illuminanceDaylight autonomySettore ING-IND/11 - Fisica Tecnica AmbientaleRenewable Energy Sustainability and the EnvironmentIlluminanceAspect ratio (image)daylight indexLight pipesHorizontal position representationRadianceEnvironmental scienceVariable numberoptimizationEnergy (miscellaneous)Marine engineering
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In vitro evaluation of shear bond strength of orthodontic stainless steel brackets using transillumination

2018

Background The objective of this study was to compare the effect of transillumination techniques to conventional light curing on shear bond strength (SBS) and adhesive remnant index (ARI) of orthodontic stainless steel brackets. Material and Methods 240 extracted human maxillary incisors, canines and premolars were randomly separated into four control and four experimental groups, based on tooth type. Labio-lingual thickness was measured. Control groups were light cured from buccal surface and experimental groups from lingual surface (transillumination) from four directions (mesial-distal, incisal-direct, direct, mesial-distal-incisal). SBS was measured using an Instron machine and ARI eval…

OrthodonticsMaterials scienceResearchBracketOrthodonticsTransillumination:CIENCIAS MÉDICAS [UNESCO]Shear bondLight curingstomatognathic diseasesstomatognathic systemBuccal SurfaceAdhesive remnant indexUNESCO::CIENCIAS MÉDICASLight cureMaxillary central incisorGeneral DentistryJournal of Clinical and Experimental Dentistry
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PALERMO SOTTO UNA “NUOVA LUCE” Sulla valorizzazione del patrimonio architettonico

2014

Today there is a greater interest in spreading the knowledge of the values of cultural heritage and the transmission of these values to a wide audience. Effective artificial lighting projects in architectural landmarks of the city of Palermo should be aimed at enhancing the cultural heritage while respecting the identity of the places, mainly thanks to technological innovations that have expanded a lot of expressive possibilities. Through the light is possible the vision of a historic building, or an ancient context, conveying the same sense of past events that have seen the construction and the events of which he was a witness.

Palermo cultura valorizzazione illuminazioneSettore ICAR/12 - Tecnologia Dell'Architettura
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Structured-light imaging through scattering

2016

We present a structured illumination technique to image objects hidden beneath scattering. The sample is computationally retrieved from a known ensemble of light patterns codified onto a digital micromirror device and photocurrent fluctuations provided by a detector with no spatial resolution. Results of laboratory experiments will be shown. Article not available.

PhotocurrentPhysicsScatteringbusiness.industryDetectorPhysics::OpticsStructured illuminationSample (graphics)Digital micromirror devicelaw.inventionOpticslawbusinessImage resolutionStructured lightImaging and Applied Optics 2016
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Tunable structured illumination system based on a Wollaston prism

2018

Experimental verification of a simple illumination system to generate a 1D structured pattern with tunable modulation frequency is shown based on a Wollaston prism illuminated by the diffracted field of an incoherent linear source.

PhysicsDiffractionbusiness.industryStructured illumination microscopyPhysics::OpticsWollaston prismStructured illuminationlaw.inventionLED lampOpticslawSpatial frequencybusinessFrequency modulationImaging and Applied Optics 2018 (3D, AO, AIO, COSI, DH, IS, LACSEA, LS&C, MATH, pcAOP)
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Whither the species? Phytophthora taxa, MOTUs and barcodes in the world of metagenomics

2015

The study of ecosystem diversity is being transformed by high throughput sequencing technology that allows an unparalleled depth of sampling of DNA barcode sequences. The opportunities are great at this fascinating interface of pathology, ecology, taxonomy and molecular biology but there are pitfalls. It is important to consider the potential for bias at all steps in the process from sampling through to data analysis. Increasingly Phytophthora diversity is being examined by metabarcoding of the PCR amplified rDNA ITS regions from soil, water or plant sample DNA. We have applied baiting and isolation, cloning and Sanger sequencing and Illumina MiSeq analysis to a time-series of filtered wate…

Phytophthora spp. metabarcoding MiSeq Illumina water samples
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