Search results for "insertion"
showing 10 items of 393 documents
Perlecan Maintains the Integrity of Cartilage and Some Basement Membranes
1999
Perlecan is a heparan sulfate proteoglycan that is expressed in all basement membranes (BMs), in cartilage, and several other mesenchymal tissues during development. Perlecan binds growth factors and interacts with various extracellular matrix proteins and cell adhesion molecules. Homozygous mice with a null mutation in the perlecan gene exhibit normal formation of BMs. However, BMs deteriorate in regions with increased mechanical stress such as the contracting myocardium and the expanding brain vesicles showing that perlecan is crucial for maintaining BM integrity. As a consequence, small clefts are formed in the cardiac muscle leading to blood leakage into the pericardial cavity and an ar…
Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system.
1998
ABSTRACT In Escherichia coli the genes encoding the anaerobic fumarate respiratory system are transcriptionally regulated by C 4 -dicarboxylates. The regulation is effected by a two-component regulatory system, DcuSR, consisting of a sensory histidine kinase (DcuS) and a response regulator (DcuR). DcuS and DcuR are encoded by the dcuSR genes (previously yjdHG ) at 93.7 min on the calculated E. coli map. Inactivation of the dcuR and dcuS genes caused the loss of C 4 -dicarboxylate-stimulated synthesis of fumarate reductase ( frdABCD genes) and of the anaerobic fumarate-succinate antiporter DcuB ( dcuB gene). DcuS is predicted to contain a large periplasmic domain as the supposed site for C 4…
Optimized recombinant dense bodies of human cytomegalovirus efficiently prime virus specific lymphocytes and neutralizing antibodies without the addi…
2010
Control of human cytomegalovirus (HCMV) infection correlates with the reconstitution of antiviral T lymphocytes in haematopoietic stem cell transplant recipients. A vaccine to foster this reconstitution and to ameliorate the severe consequences of HCMV reactivation is yet unavailable. This work focused on providing a rationale for the amendment of the yields and the antigenic composition of a vaccine, based on subviral dense bodies (DB) of HCMV. Modified DB were generated that contained the HLA-A2 presented IE1 model peptide TMYGGISLL, integrated at different positions in the major DB protein pp65. Insertion at position W175 of pp65 allowed efficient formation of recDB in the cytoplasm of i…
N-protonated 2-pyridylnickel(II) complexes insertion of isocyanides into the nickel2-pyridyl bond
1987
The reaction of the binuclear complex [NiCl(μ-2-py)(PPh3)]2 (μ-2-py = μ-C5H4N-C2,N) with the phosphines L (L = PPh3, PMePh2, PMe2Ph or PEt3) or dppe (= 1,2-bis(diphenylphosphino)ethane) in the presence of HClO4 yields the N-protonated 2-pyridyl derivatives trans-[NiCl(2-pyH)(L)2]ClO4 or [NiCl(2-pyH)(dppe)] ClO4 (2-pyH = C5H5N-C2) with a square-planar coordination around the nickel(II) center. These products are largely associated through hydrogen bonding between the NH group and the perchlorate anion, both in the solid and in chlorinated solvents. The configuration in solution has been studied by 1H and 31P NMR spectroscopy. In the complex trans-[NiCl(2-pyH)(PMe2Ph)2]ClO4, the planar 2-pyH…
Genetic rearrangement of the atzAB atrazine-degrading gene cassette from pADP1::Tn5 to the chromosome of Variovorax sp. MD1 and MD2
2007
International audience; We report the characterization of the rearrangement phenomena responsible for the movement of the atrazine-degrading atzA and B genes from pADP1::Tn5 to the chromosome of Variovorax sp. MD1 and MD2. Long PCRs and Southern blot analyses revealed that the two genes forming a gene cassette moved in a unique rearrangement event. It also revealed that the boundaries of the plasmid sequence inserted in the chromosome correspond to IS1071or to sequences close to IS1071. It suggests that this genetic rearrangement could result from the transposition of the composite transposon delimited by IS1071 insertion sequences and containing atzA and atzB genes. In addition, for MD1 an…
Characterization of a new, nonpathogenic mutant of Botrytis cinerea with impaired plant colonization capacity.
2006
International audience; Botrytis cinerea is a necrotrophic pathogen that attacks more than 200 plant species.Here, the nonpathogenic mutant A336, obtained via insertional mutagenesis, was characterized.Mutant A336 was nonpathogenic on leaves and fruits, on intact and wounded tissue, while still able to penetrate the host plant. It grew normally in vitro on rich media but its conidiation pattern was altered. The mutant did not produce oxalic acid and exhibited a modified regulation of the production of some secreted proteins (acid protease 1 and endopolygalacturonase 1). Culture filtrates of the mutant triggered an important oxidative burst in grapevine ( Vitis vinifera ) suspension cells, a…
III. Darstellung und molekülstruktur von triazoboracyclooctanen
1976
Abstract The aminoboration of phenylisocyanate or phenylisothiocyanate with 2-phenyl-1,3,2-diazabora-cyclohexane yields under ring expansion the eight-membered BNC-heterocycles 2,3-diphenyl-1,3,5-triaza-2-bora-cyclooctane-4-one or 2,3-diphenyl-1,3,5-triaza-2-bora-cyclooctane-4-thione, respectively. Isomeric structures, which can appear in the insertion reaction, are discussed on the basis of spectroscopic data.
Darstellung eines neuen achtgliedrigen BNC-heteroringes
1975
Abstract 2-Phenyl-1,3,2-diazaboracyclohexane reacts with phenylisocyanate by an insertion reaction leading to an eight-membered BNC-heterocycle.
L'entrée des jeunes dans la vie active en Bourgogne debut 2008
2009
Pour connaitre le devenir des jeunes, le ministère de l'Éducation nationale réalise chaque année une enquête avec l'appui des établissements scolaires et des centres de formation publics et privés. Cette note présente les résultats de la dernière interrogation menée en février 2008, et donne un aperçu de la diversité des premiers pas des anciens élèves et apprentis sur le marché du travail. En Bourgogne, 12500 jeunes agés de 21 ans en moyenne, ont été interrogés sur leur situation professionnelle à la sortie des formations professionnelles ou technologiques, de lycée ou de centre de formation d'apprentis.
Des compétences artistiques aux compétences professionnelles
2010
Les écoles supérieures d'art certifient des qualifications complexes et aux devenirs imprédictibles. Les études sur l'insertion professionnelle des diplômés des écoles d'art (DNSEP) menées jusqu'ici se sont attachées à décrire la qualité de leur insertion. Dans cette recherche, la réflexion porte sur la valorisation des compétences acquises en formation dans le contexte d'exercice professionnel. Les diplômés des promotion 2003 et 2005 occupant un emploi artistique au moment de l'enquête sont ainsi réinterrogés 4 à 6 ans après leur sortie de formation.