Search results for "liquid"
showing 10 items of 4351 documents
Synthesis of recombinant atrial natriuretic peptide (rANP) using hybrid fusion protein-phage fr coat/ANP (CP/ANP).
1997
Abstract Baumanis, V., I. Jansone, A. Skangals, I. Mandrika and V. Berzins. Synthesis of recombinant atrial natriuretic peptide (rANP) using hybrid fusion protein-phage fr coat/ANP (CP/ANP). Peptides 18(8) 1229–1235, 1997.—Recombinant atrial natriuretic peptide (rANP) was expressed in and isolated from E. coli. rANP was purified using HPLC. Amino acid analysis, partial sequencing, and molecular mass were determined. Fused protein was used to rise polyclonal antibodies and to develop of immunoenzymatic assays of rANP and CP/ANP. Experiments were designed to study rANP effects on isolated rabbit aortic strips and to examine hypotensive, diuretic, and natriuretic activity, as well as renal cre…
ATP distribution and localization of mitochondria in Suberites domuncula (Olivi 1792) tissue
2011
SUMMARY The metabolic energy state of sponge tissue in vivo is largely unknown. Quantitative bioluminescence-based imaging was used to analyze the ATP distribution of Suberites domuncula (Olivi 1792) tissue, in relation to differences between the cortex and the medulla. This method provides a quantitative picture of the ATP distribution closely reflecting the in vivo situation. The obtained data suggest that the highest ATP content occurs around channels in the sponge medulla. HPLC reverse-phase C-18, used for measurement of ATP content, established a value of 1.62 μmol ATP g–1 dry mass in sponge medulla, as opposed to 0.04 μmol ATP g–1 dry mass in the cortex, thus indicating a specific and…
Detection of a plant enzyme exhibiting chlorogenate-dependant caffeoyltransferase activity in methanolic extracts of arbuscular mycorrhizal tomato ro…
2012
When Glomus intraradices-colonised tomato roots were extracted in methanol at 6 degrees C, chlorogenic acid (5-caffeoylquinic acid), naturally present in the extract, was slowly converted by transesterification into methyl caffeate. The progress of the reaction could be monitored by HPLC. The reaction only occurred when the ground roots were left in contact with the hydro-alcoholic extract and required the presence of 15-35% water in the mixture. When the roots were extracted in ethanol, chlorogenic acid was transformed to ethyl caffeate in the same conditions. The reaction was also detected in Glomus mosseae-colonised tomato root extracts. It was also detectable in non-mycorrhizal root ext…
Modulation of the Biological Activity of a Tobacco LTP1 by Lipid Complexation
2004
Plant lipid transfer proteins (LTPs) are small, cysteine-rich proteins secreted into the extracellular space. They belong to the pathogenesis-related proteins (PR-14) family and are believed to be involved in several physiological processes including plant disease resistance, although their precise biological function is still unknown. Here, we show that a recombinant tobacco LTP1 is able to load fatty acids and jasmonic acid. This LTP1 binds to specific plasma membrane sites, previously characterized as elicitin receptors, and is shown to be involved in the activation of plant defense. The biological properties of this LTP1 were compared with those of LTP1-linolenic and LTP1-jasmonic acid…
Solid Contact Potentiometric Sensors Based on a New Class of Ionic Liquids on Thiacalixarene Platform
2018
New solid-contact potentiometric sensors have been developed for hydrogen phosphate recognition on the basis of ionic liquids containing tetrasubstituted derivatives of thiacalix[4]arene in cone and 1,3-alternate conformations with trimethyl- and triethylammonium fragments at the lower rim substituents. The recognition of selected anions including carbonate, hydrogen phosphate, perchlorate, oxalate, picrate, and EDTA was conducted using electrochemical impedance spectroscopy with ferricyanide redox probe. For the potentiometric sensor assembling, the ionic liquids were stabilized by multiwalled carbon nanotubes and carbon black deposited on the glassy carbon electrode. The influence of supp…
Identification et individualisation des biens revendiqués. Le sertissage de pierres et leur incorporation dans des bijoux rendant impossible toute in…
2010
International audience; (Com. 20 oct. 2009, pourvoi n° 08-20.381, arrêt n° 928-F-D, Sté Bosman c/ Sté Saro)
Revendication. « Contrat de confiés » de pierres précieuses. Revendication des pierres ayant été « ouvrées ». Usages de la profession. Preuve de la s…
2000
International audience; (Com. 6 juill. 1999, SA Joaillerie Bosman c/ SARL Saro et autres)
Axial dispersion model for solid flow in liquid suspension in system of two mixers in total recycle
2006
The measurement of residence time distribution of solid particles in solid-liquid suspension is experimentally difficult. However, the twin system approach is particularly suited for the assessment of particle RTD in flow systems as it allows overcoming some of the usual difficulties generally encountered in this kind of measurement. Twin system consists of two vessels and external piping in total recycle. Experimental results from this system can be evaluated using Z-transforms to derive particle RTD for subsequent testing of alternative flow models. Recently, the axial dispersion model was applied using the "advection diffusion equation" (sometimes called the"diffusion with bulk flow equa…
Clause de réserve de propriété. Inclusion du bien dans un plan de cession, Créance de l'article 40 au profit du vendeur revendiquant (oui)
1994
International audience; (Com. 9 juin 1992, Sté Creusot-Loire Industrie c/, Sté Sud Marine et autres)
Réserve de propriété. Plan de cession englobant un bien vendu avec réserve de propriété, Engagement du cessionnaire de faire son affaire personnelle …
1996
International audience; (Com. 17 mai 1994, SECI c/ Creusot-Loire et autre).