Search results for "loma"
showing 10 items of 1142 documents
Māsas profesionālas lomas jaundzimušā aprūpē
2016
Bakalaura darba tēma – „Māsas profesionālas lomas jaundzimušā aprūpē’ Jaundzimušais nevar pats sevi parūpēties sakarā ar to vecākiem ir jāzin pamatprincipi par jaundzimušo aprūpi. Pētījuma mērķis: Noskaidrot māsas profesionālas lomas jaundzimušā aprūpe Pētījuma uzdevumi: 1.Analizēt literatūru un citus materiālus par jaundzimušo perinatālās aprūpes principiem. 2.Izstrādāt pētniecības darba instrumentu – aptaujas anketu un veikt anketēšanu. 3. Analizēt Dorotejas Oremas pašaprūpes trūkuma teoriju 4. Apkopot un analizēt iegūtos datus. 5. Izdarīt secinājumus Pētījuma hipotēze: Vecāku izglītošana ir visnozīmīgāka māsas loma jaundzimušā aprūpē. Darbs sastāv no dīvām nodaļām. Pirmajā nodaļā ir veik…
Vecāku izglītošana par papilomas vīrusa vakcināciju pusaudzēm
2021
Bakalaura darba tēma: „Vecāku izglītošana par papilomas vīrusa vakcināciju pusaudzēm”. Tēmas aktualitāti nosaka tas, ka viens no galvenajiem iemesliem, pusaudzes vakcinācijas atteikuma gadījumiem ir vecāku izglītošanas trūkums par vakcīnu un vakcināciju. Pētījuma mērķis: izpētīt vecāku izglītošanu par papilomas vīrusa vakcināciju pusaudzēm. Pētniecības uzdevumi: apskatīt, apkopot un analizēt nepieciešamo zinātnisko literatūru par vecāku izglītošanu pret papilomas vīrusa vakcināciju un analizēt B. Ņūmenas veselības aprūpes sistēmas modeli, izmatojot to pētniecības darbā. Lai veiktu pētniecisko darbu, tika izmantota kvantitatīva pētījuma metode, pētījuma instruments – anketa. Pētījuma iegūtie…
Developing EU Heritage Diplomacy : Notions of Cultural Diplomacy, Cultural Heritage, and Intercultural Dialogue among EU Officials and European Herit…
2021
The European Commission has recently identified cultural heritage as a focus area for EU cultural diplomacy. The article explores EU cultural diplomacy that deals with cultural heritage and develops the concept of heritage diplomacy based on a critical discourse analysis of interviews with EU officials and heritage practitioners working at sites awarded with the European Heritage Label. What do these actors mean by cultural diplomacy and how do they understand the role and potential of cultural heritage for it and with what effects? The analysis indicates that heritage diplomacy means different things for EU officials and heritage practitioners. Their discourses on the uses of cultural heri…
Transfection of lipoma cells with papilloma bovine virus subgenomic fragment.
1991
Abstract Lipoma cells with consistent chromosomal aberration have been transfected with plasmids carrying papilloma bovine virus subgenomic fragment (PBV 69). The succesful transformation of the cells was ascerted on the changed growth pattern of the cells in liquid medium, colony formation in soft agar and modified cell appearrance in electron microscopy; transfection with PBV 69 has not been, however, sufficient to immortalize lipoma cells.
Binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells
1995
Infection of cells by human papillomaviruses (HPVs) associated with malignant genital lesions has not been studied because of the lack of an in vitro system and the unavailability of virions. We have now used virus-like particles (VLPs) of HPV type 33 to analyze the initial events in the interaction of the HPV capsid with cell lines. Binding of VLPs to HeLa cells was observed in biochemical assays and by immunofluorescence. VLP binding was inhibited by antisera raised against VLPs but not by monoclonal antibodies recognizing either L1 or L2 epitopes accessible on VLPs. Under saturating conditions, approximately 2 x 10(4) VLPs were bound per cell, with a dissociation constant of about 100 pM…
Assembly and Translocation of Papillomavirus Capsid Proteins
2002
ABSTRACT The major and minor capsid proteins of polyomavirus are preassembled in the cytoplasm and translocated to the nucleus only as a VP1-VP2/VP3 complex. In this study, we describe independent nuclear translocation of the L1 major protein and the L2 minor capsid protein of human papillomavirus type 33 by several approaches. First, we observed that expression and nuclear translocation of L2 in natural lesions precede expression of L1. Second, using a cell culture system for coexpression, we found that accumulation of L2 in nuclear domain 10 (ND10) subnuclear structures precedes L1 by several hours. In contrast, complexes of L2 and mutants of L1 forced to assemble in the cytoplasm are tra…
Nuclear localization but not PML protein is required for incorporation of the papillomavirus minor capsid protein L2 into virus-like particles.
2004
ABSTRACT Recent reports suggest that nuclear domain(s) 10 (ND10) is the site of papillomavirus morphogenesis. The viral genome replicates in or close to ND10. In addition, the minor capsid protein, L2, accumulates in these subnuclear structures and recruits the major capsid protein, L1. We have now used cell lines deficient for promyelocytic leukemia (PML) protein, the main structural component of ND10, to study the role of this nuclear protein for L2 incorporation into virus-like particles (VLPs). L2 expressed in PML protein knockout (PML −/− ) cells accumulated in nuclear dots, which resemble L2 aggregates forming at ND10 in PML protein-containing cells. These L2 assemblies also attracted…
Generation and neutralization of pseudovirions of human papillomavirus type 33
1997
Since human papillomaviruses (HPV) cannot be propagated in cell culture, the generation of infectious virions in vitro is a highly desirable goal. Here we report that pseudovirions can be generated by the assembly of virus-like particles (VLPs) in COS-7 cells containing multiple copies of a marker plasmid. Using recombinant vaccinia viruses, we have obtained spherical VLPs of HPV type 33 (HPV-33) which fractionate into heavy and light VLPs in cesium chloride density gradients. VLPs in the heavy fraction (1.31 g/cm3) carry the plasmid in DNase-resistant form and are capable of transferring the genetic marker located on the plasmid to COS-7 cells in a DNase-resistant way (pseudoinfection). Th…
DNA-induced structural changes in the papillomavirus capsid.
2001
ABSTRACT Human papillomavirus capsid assembly requires intercapsomeric disulfide bonds between molecules of the major capsid protein L1. Virions isolated from naturally occurring lesions have a higher degree of cross-linking than virus-like particles (VLPs), which have been generated in eukaryotic expression systems. Here we show that DNA encapsidation into VLPs leads to increased cross-linking between L1 molecules comparable to that seen in virions. A higher trypsin resistance, indicating a tighter association of capsomeres through DNA interaction, accompanies this structural change.
Human Papillomavirus Types 16, 18, and 31 Share Similar Endocytic Requirements for Entry
2013
ABSTRACT Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.