Search results for "microbiology"
showing 10 items of 7546 documents
Involvement of osmotic cell shrinkage on the proton extrusion rate in Saccharomyces cerevisiae
2001
Saccharomyces cerevisiae has been subjected to hyperosmotic shocks by using permeating (sorbitol, xylitol, glycerol, NaCl) and nonpermeating (PEG 600) solutes. The proton extrusion rate decreased as the osmotic pressure increased, whichever solute was used. However, the total inhibition of the cellular H+ extrusion depended on the solute used. A total inhibition was observed at about 20 MPa with glycerol, xylitol and sorbitol. With PEG 600, a total inhibition of extracellular acidification was obtained at 8.5 MPa. NaCl, with an extracellular pressure of 37.8 MPa (near saturation), did not completely inhibit the extracellular acidification. These results showed that the total inhibition of p…
Carbohydrate dynamics in particle-size fractions of sandy spodosols following forest conversion to maize cropping
2006
International audience; In southwest France, much of the forested land on sandy spodosols has been converted to continuous maize cropping in the last few decades. To evaluate the impacts of this change on soil organic matter properties, we compared total organic C and neutral and amino sugars content in whole soil and particle size separates of two forested, and five related sites that had been either clear-cut for 12 and 18 mo, or cultivated to maize for 4-22yr. Soil carbohydrates accounted for 4-7% of the total organic C across all sites. Soil organic C contents of clear-cut and cultivated sites were only 57-79% of the average value measured in forested sites. Accordingly, carbohydrate co…
Bacterial rhamnolipids are novel MAMPs conferring resistance to Botrytis cinerea in grapevine
2009
International audience; Rhamnolipids produced by the bacteria Pseudomonas aeruginosa are known as very efficient biosurfactant molecules. They are used for a wide range of industrial applications, especially in food, cosmetics and pharmaceutical formulations as well as in bioremediation of pollutants. In this paper, the role of rhamnolipids as novel molecules triggering defence responses and protection against the fungus Botrytis cinerea in grapevine is presented. The effect of rhamnolipids was assessed in grapevine using cell suspension cultures and vitro-plantlets. Ca2+ influx, mitogen-activated protein kinase activation and reactive oxygen species production form part of early signalling…
Laurel extracts inhibit quorum sensing, virulence factors and biofilm of foodborne pathogens
2020
Antimicrobial, antibiofilm, anti-Quorum sensing (QS) and virulence factors inhibitory capacity of different polarity Laurus nobilis extracts against several pathogenic microorganisms were studied. Some extracts exhibited antibiotic effect against Staphylococcus aureus multidrug-resistant strains. However, all extracts (100 µg/mL) inhibited to some extent the biofilm of most bacteria tested (until 40% for Gram-negative and 76% for Gram-positive). Hexane (HE) and chloroform extract (CE) were potent inhibitors of S. aureus biofilm and the microscopies further confirmed an important reduction in adherent cells. Polystyrene surfaces coated with these extracts showed a decrease in bacterial adhes…
First evidence of underwater sounds emitted by the living fossils Lepidurus lubbocki and Triops cancriformis (Branchiopoda: Notostraca)
2021
Sound is the most effective means of communication in marine and freshwater ecosystems. However, no data about acoustic emissions from non-malacostracan crustaceans are currently available, so their ability to produce sounds is unknown. For the first time, this study investigated the sound produced by 2 tadpole shrimp species,Triops cancriformisandLepidurus lubbocki.L. lubbockiindividuals were collected from a natural temporary pond in Sicily (Italy), whereasT. cancriformisindividuals were obtained from eggs contained in sediment from a rock pool in Sardinia (Italy). In the laboratory, experimental tanks with the animals (one species at a time) were acoustically monitored. Both species prod…
Experimental climate warming alters the relationship between fungal root symbiosis and Sphagnum litter phenolics in two peatland microhabitats
2017
International audience; Belowground interactions between plants and microorganisms are involved in numerous ecosystems processes such as carbon and nutrient cycling. Understanding their responses to on-going climate warming is thus of paramount importance to better predict future ecosystem functioning. We hypothesized that climate warming alters the interactions between Sphagnum litter phenolics and the fungal root symbiosis of the Ericale plant Andromeda polifolia in a Jura mountain peatland (France). We initiate a climate warming treatment (+1°C) in April 2008 in two microhabitats (lawns and hummocks). We measured polyphenolic contents, mycorrhizal and dark septate endophyte (DSE) root co…
Mycotheca of Edible and Medicinal Mushrooms at Herbarium SAF as a Potential Source of Nutraceuticals and Cultivated Mushrooms
2018
Basidiomycetes strains (n = 39) belonging to 9 genera in 8 families are kept in the mycotheca of the Department of Agricultural, Food and Forest Sciences at the University of Palermo (Palermo, Italy). All of the strains are medicinal mushrooms, and some are of great commercial and nutraceutical interest.
The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholde…
2014
International audience; The role of flagellin perception in the context of plant beneficial bacteria still remains unclear. Here, we characterized the flagellin sensing system flg22-FLAGELLIN SENSING 2 (FLS2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth-promoting rhizobacterium (PGPR) Burkholderia phytofirmans. The functionality of the grapevine FLS2 receptor, VvFLS2, was demonstrated by complementation assays in the Arabidopsis thaliana fls2 mutant, which restored flg22-induced H2O2 production and growth inhibition. Using synthetic flg22 peptides from different bacterial origins, we compared recognition specificities between VvFLS2…
An STE12 gene identified in the mycorrhizal fungus Glomus intraradices restores infectivity of a hemibiotrophic plant pathogen
2009
International audience; * • Mechanisms of root penetration by arbuscular mycorrhizal (AM) fungi are unknown and investigations are hampered by the lack of transformation systems for these unculturable obligate biotrophs. Early steps of host infection by hemibiotrophic fungal phytopathogens, sharing common features with those of AM fungal colonization, depend on the transcription factor STE12. * • Using degenerated primers and rapid amplification of cDNA ends, we isolated the full-length cDNA of an STE12-like gene, GintSTE, from Glomus intraradices and profiled GintSTE expression by real-time and in situ RT-PCR. GintSTE activity and function were investigated by heterologous complementation …
Symbiosis-related plant genes modulate molecular responses in an arbuscular mycorrhizal fungus during early root interactions.
2009
To gain further insight into the role of the plant genome in arbuscular mycorrhiza (AM) establishment, we investigated whether symbiosis-related plant genes affect fungal gene expression in germinating spores and at the appressoria stage of root interactions. Glomus intraradices genes were identified in expressed sequence tag libraries of mycorrhizal Medicago truncatula roots by in silico expression analyses. Transcripts of a subset of genes, with predicted functions in transcription, protein synthesis, primary or secondary metabolism, or of unknown function, were monitored in spores and germinating spores and during interactions with roots of wild-type or mycorrhiza-defective (Myc–) mutan…