Search results for "minion"
showing 10 items of 11 documents
Real-time detection of BRAF V600E mutation from archival hairy cell leukemia FFPE tissue by nanopore sequencing
2018
The MinION is a miniaturized high-throughput next generation sequencing platform of novel conception. The use of nucleic acids derived from formalin-fixed paraffin-embedded samples is highly desirable, but their adoption for molecular assays is hurdled by the high degree of fragmentation and by the chemical-induced mutations stemming from the fixation protocols. In order to investigate the suitability of MinION sequencing on formalin-fixed paraffin-embedded samples, the presence and frequency of BRAF c.1799T > A mutation was investigated in two archival tissue specimens of Hairy cell leukemia and Hairy cell leukemia Variant. Despite the poor quality of the starting DNA, BRAF mutation was su…
Assembly methods for nanopore-based metagenomic sequencing: a comparative study
2019
ABSTRACTBackgroundMetagenomic sequencing has lead to the recovery of previously unexplored microbial genomes. In this sense, short-reads sequencing platforms often result in highly fragmented metagenomes, thus complicating downstream analyses. Third generation sequencing technologies, such as MinION, could lead to more contiguous assemblies due to their ability to generate long reads. Nevertheless, there is a lack of studies evaluating the suitability of the available assembly tools for this new type of data.FindingsWe benchmarked the ability of different short-reads and long-reads tools to assembly two different commercially available mock communities, and observed remarkable differences i…
Animales, teorías feministas posmodernas y prácticas artísticas.
2019
Este artículo pretende mostrar cómo algunas teorías feministas posmodernas como el ecofeminismo y el posthumanismo, de autoras como Françoise d'Eaubonne, Alicia Puleo, Donna Haraway o Rosi Baridotti han contribuido a la crítica contra las prácticas androcéntricas basadas en el dominio sistémico de devastación y explotación del otro/a/os, perpetuando la jerarquía y autoridad del hombre. Frente a ello estas autoras han propuesto un nuevo modelo de relación orgánica y de convivencia entre cultura y naturaleza, capaz de superar los modelos binarios, inspirado en el respeto a la diversidad de sexos y especies.Estos principios se hayan, así mismo, en las prácticas artísticas de algunas autoras c…
Targeted sequencing of BRAF by MinION in archival Formalin-Fixed Paraffin-Embedded specimens allows to discriminate between Hairy Cell Leukemia and H…
2016
Targeted sequencing of BRAF by MinION in archival Formalin-Fixed Paraffin-Embedded specimens allows to discriminate between Hairy Cell Leukemia and Hair Cell Leukemia Variant
Iniuria Migrandi: Criminalization of Immigrants and the Basic Principles of the Criminal Law
2013
In questo articolo mi propongo di effettuare una valutazione normativa, dal punto di vista di una teoria del diritto penale orientata secondo principi, delle norme che incriminano l'immigrazione irregolare. L'interrogativo generale sul quale mi soffermerò è questo: che modo di usare il diritto penale è implicato nella emanazione di simili norme? La mia tesi è, in sintesi, che si tratta di un vero e proprio abuso del diritto penale. In almeno due sensi: primo, nel senso che, criminalizzando l'immigrazione irregolare, il diritto penale bandisce (categorie di) persone, e non atti/omissioni, facendo qualcosa che un diritto penale rispettoso di alcuni principi liberali fondamentali non dovrebbe …
Direct RNA nanopore sequencing of SARS-CoV-2 extracted from critical material from swabs
2020
ABSTRACTBackgroundIn consideration of the increasing prevalence of COVID-19 cases in several countries and the resulting demand for unbiased sequencing approaches, we performed a direct RNA sequencing experiment using critical oropharyngeal swab samples collected from Italian patients infected with SARS-CoV-2 from the Palermo region in Sicily.MethodsHere, we identified the sequences SARS-CoV-2 directly in RNA extracted from critical samples using the Oxford Nanopore MinION technology without prior cDNA retro-transcription.ResultsUsing an appropriate bioinformatics pipeline, we could identify mutations in the nucleocapisid (N) gene, which have been reported previously in studies conducted in…
AIs for Dominion Using Monte-Carlo Tree Search
2015
Dominion is a complex game, with hidden information and stochastic elements. This makes creating any artificial intelligence AI challenging. To this date, there is little work in the literature on AI for Dominion, and existing solutions rely upon carefully tuned finite-state solutions. This paper presents two novel AIs for Dominion based on Monte-Carlo Tree Search MCTS methods. This is achieved by employing Upper Confidence Bounds UCB and Upper Confidence Bounds applied to Trees UCT. The proposed solutions are notably better than existing work. The strongest proposal is able to win 67% of games played against a known, good finite-state solution, even when the finite-state solution has the u…
Minions. Felicemente irresponsabili
2015
Articolo della serie di analisi dei cartoni animati rivolti alla prima infanzia.
An AI for dominion based on Monte-Carlo methods
2014
Masteroppgave i Informasjons- og kommunikasjonsteknologi IKT590 Universitetet i Agder 2014 To the best of our knowledge there exists no Arti_cial Intelligence (AI)for Dominion which uses Monte Carlo methods, that is competitive on ahuman level. This thesis presents such an AI, and tests it against someof the top Dominion strategies available. Although in a limited testingenvironment, the results show that our AI is capable of competing withhuman players, while keeping processing time per move at an acceptablelevel for human players. Although the approach for our AI is built onprevious knowledge about Upper Con_dence Bounds (UCB) and UCBapplied to Trees (UCT), an approach for handling the st…
Direct RNA Nanopore Sequencing of SARS-CoV-2 Extracted from Critical Material from Swabs
2022
In consideration of the increasing prevalence of COVID-19 cases in several countries and the resulting demand for unbiased sequencing approaches, we performed a direct RNA sequencing (direct RNA seq.) experiment using critical oropharyngeal swab samples collected from Italian patients infected with SARS-CoV-2 from the Palermo region in Sicily. Here, we identified the sequences SARS-CoV-2 directly in RNA extracted from critical samples using the Oxford Nanopore MinION technology without prior cDNA retrotranscription. Using an appropriate bioinformatics pipeline, we could identify mutations in the nucleocapsid (N) gene, which have been reported previously in studies conducted in other countri…