Search results for "modified"

showing 10 items of 585 documents

Molecular architecture and activation of the insecticidal protein Vip3Aa from Bacillus thuringiensis

2020

9 p.-5 fig.

Models Molecular0301 basic medicineProteasesBiologiaMolecular biologymedicine.medical_treatmentScienceAmino Acid MotifsBacillus thuringiensisGeneral Physics and Astronomy02 engineering and technologyGenetically modified cropsBiotecnologiaArticleProtein Structure SecondaryGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesBacterial ProteinsProtein DomainsTetramerBacillus thuringiensisElectron microscopymedicineTrypsinlcsh:ScienceMultidisciplinaryProteasebiologyChemistryQfungifood and beveragesMidgutGeneral Chemistry021001 nanoscience & nanotechnologybiology.organism_classification030104 developmental biologyStructural biologyBiochemistrylcsh:QStructural biology0210 nano-technologyProteïnesFunction (biology)
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Ultimate Shear of RC Beams with Corroded Stirrups and Strengthened with FRP

2019

Transverse reinforcement plays a key role in the response behavior of reinforced concrete beams. Therefore, corrosion of steel stirrups may change the failure mode of elements from bending to shear, leading to a brittle and catastrophic crisis. It is important to strengthen reinforced concrete beams with corroded stirrups to enhance the shear resistance. This paper presents a formulation, based on the modified compression field theory, to estimate the ultimate shear of reinforced concrete beams strengthened with FRP, because of stirrup corrosion. The detrimental effect of corrosion on steel stirrup yield strength was taken into account by introducing an empirical decay law. The effective st…

Modified Compression Field TheoryBeams; Corrosion; FRP; MCFT; Parametric analysis; Reinforced concrete; Shear; Stirrups; Civil and Structural Engineering; Building and ConstructionMaterials science0211 other engineering and technologies020101 civil engineering02 engineering and technologyshearstirrupslcsh:TH1-97450201 civil engineeringCorrosioncorrosion; stirrups; shear; FRP; MCFT; reinforced concrete; beams; parametric analysisBrittleness021105 building & constructionArchitectureMCFTCivil and Structural EngineeringEnvironmental Science (all)corrosion2300Parametric analysibusiness.industryBeamStructural engineeringBuilding and ConstructionFibre-reinforced plasticBeams; Corrosion; FRP; MCFT; Parametric analysis; Reinforced concrete; Shear; Stirrups; Architecture; 2300; Environmental Science (all); Civil and Structural Engineering; Building and ConstructionReinforced concretereinforced concreteStirrupShear (geology)parametric analysisbeamsbusinessFailure mode and effects analysisFRPlcsh:Building constructionBuildings; Volume 9; Issue 2; Pages: 34
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Nonlinear Analysis of Beams Reinforced in Shear with Stirrups and Steel Fibers

2012

The modified compression field theory (MCFT) and the disturbed stress field model (DSFM) are often used to predict the nonlinear behavior of reinforced concrete structures. This study presents several extensions of the MCFT and DSFM to the case of high-strength steel fiber-reinforced concrete beams subjected to transverse loads. Experimental four-point bending tests were conducted on 12 concrete beams with a different percentage of fibers and/or stirrups. To validate the updates introduced in the analytical models, numerical analysis was performed using nonlinear finite element software. Modeling of the post-peak softening branch of the tensile and compressive constitutive curves of fibrous…

Modified Compression Field TheoryMaterials scienceSFRC beamsbusiness.industryNumerical analysiscrackingBuilding and ConstructionFiber-reinforced concreteStructural engineeringshearFinite element methodlaw.inventionStress fieldfiber-reinforced concretenonlinear finite element model analysiSettore ICAR/09 - Tecnica Delle CostruzioniCrackingTransverse planeShear; SFRC beamslawUltimate tensile strengthexoerimental testComposite materialbusinessCivil and Structural Engineering
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Experimental Tests and FEM Model for SFRC Beams under Flexural and Shear Loads

2008

The complete load-vs-displacement curves obtained by four-point-bending tests on Steel Fiber Reinforced Concrete (SFRC) beams are predicted by using a nonlinear finite element code based on the Modified Compression Field Theory (MCFT) and the Disturbed Stress Field Model (DSFM) suitably adapted for SFRC elements. The effect of fibers on the shear-flexure response is taken into account, mainly incorporating tensile stress-strain analytical relationship for SFRC. The numerical results show the effectiveness of the model for prediction of the behavior of the tested specimens reinforced with light amount of stirrups or with fibers only. © 2008 American Institute of Physics.

Modified Compression Field TheoryMaterials scienceSFRC beamsbusiness.industryShearStructural engineeringFiber-reinforced concreteNonlinear finite element analysisFinite element methodlaw.inventionStress fieldfiber-reinforced concretePhysics and Astronomy (all)Settore ICAR/09 - Tecnica Delle Costruzionishear and flexureShear; SFRC beamsFlexural strengthShear (geology)lawExperimental testUltimate tensile strengthComposite materialbusinessFEM analysiAIP Conference Proceedings
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Effect of ageing on the morphology and creep and recovery of polymer-modified bitumens

2018

Polymer additives are used to improve the properties of road bitumens including their oxidative resistance. However, their usage as anti-oxidative materials remains relatively unclear. This study aims to investigate the changes in the morphology and the rheological response of polymer modified bitumens used in road pavement construction caused by ageing. An elastomer (radial styrene butadiene styrene, SBS) and a plastomer (ethyl vinyl acetate, EVA) polymer were mixed with one base bitumen at three polymer concentrations. The bitumens were RTFO and PAV aged. The morphology of the bitumens was captured by fluorescence microscopy while the rheological properties were measured by means of the m…

MorphologyStyrene-butadieneMaterials sciencePercent recovery0211 other engineering and technologies02 engineering and technologyElastomerPlastomerchemistry.chemical_compoundRheology021105 building & constructionSettore ICAR/04 - Strade Ferrovie Ed AeroportiGeneral Materials ScienceComposite materialPolymer-modified bitumenCivil and Structural Engineeringchemistry.chemical_classificationMSCRBuilding and ConstructionPolymer021001 nanoscience & nanotechnologyAgeingchemistryCreepMechanics of MaterialsAsphaltHardening (metallurgy)Polymer-modified bitumen Ageing Morphology Percent recovery MSCR0210 nano-technology
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The phosphorylated pathway of serine biosynthesis is essential both for male gametophyte and embryo development and for root growth in Arabidopsis.

2013

This study characterizes the phosphorylated pathway of Ser biosynthesis (PPSB) in Arabidopsis thaliana by targeting phosphoserine phosphatase (PSP1), the last enzyme of the pathway. Lack of PSP1 activity delayed embryo development, leading to aborted embryos that could be classified as early curled cotyledons. The embryo-lethal phenotype of psp1 mutants could be complemented with PSP1 cDNA under the control of Pro35S (Pro35S:PSP1). However, this construct, which was poorly expressed in the anther tapetum, did not complement mutant fertility. Microspore development in psp1.1/psp1.1 Pro35S:PSP1 arrested at the polarized stage. The tapetum from these lines displayed delayed and irregular devel…

MutantCitric Acid CycleGreen Fluorescent ProteinsImmunoblottingArabidopsisPlant ScienceBiologyPlant RootsSerineMicrosporeMicroscopy Electron TransmissionGene Expression Regulation PlantArabidopsisSerineArabidopsis thalianaAmino AcidsPhosphorylationResearch ArticlesTapetumArabidopsis ProteinsReverse Transcriptase Polymerase Chain ReactionGene Expression Regulation DevelopmentalEmbryoPhosphoserine phosphataseCell Biologybiology.organism_classificationPlants Genetically ModifiedPhosphoric Monoester HydrolasesBiosynthetic PathwaysBiochemistryMicroscopy FluorescenceMutationSeedsPollenGlycolysisThe Plant cell
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Variability in the cadherin gene in an Ostrinia nubilalis strain selected for Cry1Ab resistance

2008

Transgenic corn expressing Cry1Ab (a Bacillus thuringiensis toxin) is highly effective in the control of Ostrinia nubilalis. For its toxic action, Cry1Ab has to bind to specific insect midgut proteins. To date, in three Lepidoptera species resistance to a Cry1A toxin has been conferred by mutations in cadherin, a protein of the Lepidoptera midgut membrane. The implication of cadherin in the resistance of an Ostrinia nubilalis colony (Europe-R) selected with Bacillus thuringiensis Cry1Ab protoxin was investigated. Several major mutations in the cadherin (cdh) gene were found, which introduced premature termination codons and/or large deletions (ranging from 1383 to 1701bp). The contribution …

MutantDrug ResistanceGenetically modified cropsMothsBiologyBiochemistryOstriniaHemolysin ProteinsBacterial ProteinsBacillus thuringiensisBotanyGenetic variationAnimalsMolecular BiologyGeneGeneticsPolymorphism GeneticBacillus thuringiensis ToxinsCadherinfungiGenetic Variationfood and beveragesMidgutCadherinsbiology.organism_classificationEndotoxinsInsect ScienceMutationInsect ProteinsInsect Biochemistry and Molecular Biology
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MOZ/TIF2-induced acute myeloid leukaemia in transgenic fish.

2008

The inv(8)(p11q13) chromosomal abnormality, described in acute myeloid leukaemias (AML), fuses the histone acetyl-transferase (HAT) MYST3 (MOZ) gene with another HAT gene, NCOA2 (TIF2). We generated a transgenic zebrafish in which the MYST3/NCOA2 fusion gene was expressed under control of the spi1 promoter. An AML developed in 2 of 180 MYST3/NCOA2-EGFP-expressing embryos, 14 and 26 months after injection of the fusion gene in a one-cell embryo, respectively. This leukaemia was characterised by an extensive invasion of kidneys by myeloid blast cells. This model, which is the first zebrafish model of AML, demonstrates the oncogenic potency of MYST3/NCOA2 fusion gene.

MyeloidMicroinjectionsOncogene Proteins FusionTransgeneBiologyKidneyMYST3Fusion geneAnimals Genetically ModifiedNuclear Receptor Coactivator 2hemic and lymphatic diseasesmedicineAnimalsZebrafishGeneZebrafishHistone AcetyltransferasesSPI1Reverse Transcriptase Polymerase Chain ReactionHematologymedicine.diseasebiology.organism_classificationMolecular biologyLeukemiaDisease Models AnimalLeukemia Myeloid Acutemedicine.anatomical_structureembryonic structuresCancer researchGene FusionBritish journal of haematology
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Development of Durum Wheat Breads Low in Sodium Using a Natural Low-Sodium Sea Salt

2020

Durum wheat is widespread in the Mediterranean area, mainly in southern Italy, where traditional durum wheat breadmaking is consolidated. Bread is often prepared by adding a lot of salt to the dough. However, evidence suggests that excessive salt in a diet is a disease risk factor. The aim of this work is to study the effect of a natural low-sodium sea salt (Saltwell&reg

Na+ reductionHealth (social science)food.ingredientSalt content030309 nutrition & dieteticssensory evaluationSodiumbreadchemistry.chemical_elementphysico-chemical and textural attributesPlant Sciencelcsh:Chemical technologyHealth Professions (miscellaneous)MicrobiologyArticle03 medical and health sciences0404 agricultural biotechnologyfoodNaClDisease risk factorlcsh:TP1-1185Food sciencelow-sodium sea salt0303 health sciencesChemistryTriticum turgidum L. subsp. durum DesfSea saltdigestive oral and skin physiologyfood and beverages04 agricultural and veterinary sciencesWheat breadTriticum turgidum L. subsp. durum Desf bread NaCl low-sodium sea salt Na+ reduction physico-chemical and textural attributes sensory evaluation040401 food scienceTriticum turgidum L. subsp. durum Desf.Modified atmosphereMediterranean areaFood ScienceLow sodiumFoods
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Sensing life: regulation of sensory neuron survival by neurotrophins

2002

Neurotrophins are a family of structurally and functionally related neurotrophic factors which, in mammals, include: nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 (NT-3), and NT-4/5. In addition to their canonical role in promoting neuronal survival, these molecules appear to regulate multiple aspects of the development of the nervous system in vertebrates, including neuronal differentiation, axon elongation and target innervation, among others. Actions of neurotrophins and of their receptors in vivo are being analyzed by loss-of-function or gain-of-function experiments in mice. Here, we review the phenotypes of the primary sensory system in these mutant mouse strai…

Nervous systemGenetically modified mouseCell SurvivalMice TransgenicSensory systemReceptors Nerve Growth FactorMiceCellular and Molecular NeuroscienceNeurotrophic factorsmedicineAnimalsReceptor trkCNerve Growth FactorsNeurons AfferentAxonMolecular BiologyMice KnockoutPharmacologyMembrane GlycoproteinsbiologyBrain-Derived Neurotrophic FactorCell BiologyAnatomyProtein-Tyrosine KinasesSensory neuronmedicine.anatomical_structureNerve growth factornervous systembiology.proteinMolecular MedicineNeuroscienceSignal TransductionNeurotrophinCellular and Molecular Life Sciences
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