Search results for "nucleus"

PARTICIPATION OF RAT LIVER NUCLEI IN MOVEMENTS OF SODIUM.

1964

In vitro investigations of movements of sodium with muscular tissue seem to have shown that the non-extracellular sodium belongs to more than one phase1. The existence of either a membranous and a cellular phase2 or of two cellular phases with different degrees of order3 has been discussed. We wish to present some experimental findings which may well contribute to the interpretation of sodium transfer processes in animal tissue.

A novel SP-1 site in the human interleukin-1β promoter confers preferential transcriptional activity in keratinocytes

1996

To investigate the mechanisms of transcriptional activation of interleukin-1beta (IL-1beta) in non-monocytic cells, we constructed a series of reporter plasmids with the bacterial chloramphenicol acetyltransferase gene linked to various parts of the human IL-1beta promoter and performed transient transfection experiments. We identified a promoter segment that activates transcription most efficiently in keratinocytes. Electrophoretic mobility shift assays (EMSA) with a 43-mer oligonucleotide derived from the functionally identified cis-acting element revealed specific complexes. By competition analysis with transcription factor consensus sequence oligonucleotides and by immunosupershift, tra…

Time-related changes in size of nuclei of pinealocytes in rats

1981

In a study of 96 adult male Sprague-Dawley rats, da- and time-related changes of the mean nuclear volume of pinealocytes were determined with the aim of testing the reproducibility of the karyometric findings described by Quay and Renzoni (1966). It is shown (i) that statistically significant differences exist between the central and peripheral mean volumes of pinealocyte nuclei/group of animals and time point (p less than 0.001), (ii) that the day and time related differences are statistically different in both regions (p less than 0.001), (iii) that in the center and periphery of the pineal body different diurnal patterns exist, and (iv) that the diurnal patterns are not parallel in the t…

The autoantigen La/SSB: detection on and uptake by mitotic cells.

1992

Abstract The nuclear autoantigen La, a transcription/termination factor of RNA polymerase III, was recently shown to translocalize to the cell surface of growth-stimulated cells during transition from G0- to G1-phase. Here we describe the staining of living mitotic cells with the anti-La mab La11G7. Moreover, La protein added to cell culture medium was able to enter into synchronized mitotic cells. Uptake was inhibited by the anti-La mab. La protein taken up into prophase cells assembled into a fibrillar network. Taken up by ana/telophase cells, La protein was preferentially transported into the newly forming or formed nuclei. This import allowed us to study directly the intranuclear locali…

Characterization of a nuclear localization signal of canine parvovirus capsid proteins.

1998

We investigated the abilities of synthetic peptides mimicking the potential nuclear localization signal of canine parvovirus (CPV) capsid proteins to translocate a carrier protein to the nucleus following microinjection into the cytoplasm of A72 cells. Possible nuclear localization sequences were chosen for synthesis from CPV capsid protein sequences (VP1, VP2) on the basis of the presence of clustered basic residues, which is a common theme in most of the previously identified targeting peptides. Nuclear targeting activity was found within the N-terminal residues 4-13 (PAKRARRGYK) of the VP1 capsid protein. While replacement of Arg10 with glycine did not affect the activity, replacement of…

Binucleate cells in the Ehrlich ascites tumor. Autoradiographic labeling

1989

Abstract An autoradiographic study was performed on binucleate and mitotic cells in the Ehrlich ascites tumor (EAT) untreated and after treatment with 5-fluorouracil (FU). The number of binucleate cells was greater in the treated tumor than in the controls. It was also observed that the number of labeled mitoses was greater in the Fu-treated tumor. Autoradiographic labeling showed that the cells that proved to be binucleate had previously passed through S-phase; thus, these cells belonged to the proliferative compartment.

Rôle of the nucleus in the maintenance of the protein level in the algaAcetabularia mediterranea

1954

Sono state studiate le variazioni del rapporto tra l'N proteico e non proteico in segmenti rigeneranti, nucleati ed anucleati, diAcetabul. medit. I risultati ottenuti hanno dimostrato che il predetto rapporto aumenta in entrambi i segmenti il primo giorno dopo l'operazione. Nei giorni seguenti esso diminuisce in entrambi i segmenti, ma piu sensibilmente in quelli anucleati, nei quali va al di sotto del valore medio delle alghe normali gia dopo il 3°–4° giorno.

A Comparison of the Histodynamics of Sebaceous Glands and Epidermis in Man: a Microanatomic and Morphometric Study

1974

Temporal speciation pattern in the western Mediterranean genus Tudorella P. Fischer, 1885 (Gastropoda, Pomatiidae) supports the Tyrrhenian vicariance…

2009

The land snail genus Tudorella shows a peculiar disjunct distribution around the western Mediterranean coasts. Despite high phenotypic plasticity, only two species with a disputed number of subspecific taxa are currently recognised. We delimited the species with mitochondrial (COI & 16S) and nuclear (ITS-1) markers based on the unified species concept and suggested that there are eight species in the genus, two of them currently undescribed. Applying Bayesian phylogenetic model selection, we tested four different biogeographic hypotheses that could be causal for the current distribution pattern of extant Tudorella species. A scenario involving vicariance events resulting from the repeated s…

A GFP-tagged Muscleblind C protein isoform reporter construct

2010

Drosophila muscleblind (mbl), the ortholog of human Muscleblind-like 1 (MBNL1) gene involved in Myotonic Dystrophy (DM), gives raise to protein isoforms MblA to G. The specific functions and subcellular distribution of isoforms are still largely unknown. To overcome the lack of isoform-specific antibodies we generated transgenic flies that express a GFP:MblC fusion protein under the control of the Gal4/UAS system. The reporter fusion protein was able to functionally complement mbl loss of function mutations, demonstrating activity, and accumulated predominantly in adult muscle nuclei. The fluorescent nature of the reporter makes it appropriate for live imaging detection of MblC protein isof…