Search results for "peripheral blood"

showing 10 items of 349 documents

Lymphocyte subset reference values in peripheral blood: European survey

1992

Reference valuesImmunologyCell BiologyGeneral MedicineBiologyPeripheral bloodLymphocyte subsetsBiology of the Cell
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Proliferative response of synovial fluid and peripheral blood mononuclear cells to arthritogenic and non-arthritogenic microbial antigens and to the …

1990

Cellular immune responses to microbial antigens have been implicated in the pathogenesis of some forms of arthritis including reactive arthritis, Reiter's syndrome, ankylosing spondylitis and rheumatoid arthritis. We investigated the proliferative T cell responses of paired peripheral blood (PB) and synovial fluid (SF) mononuclear cells (MC) to so-called arthritogenic bacteria (Yersinia enterocolitica and Salmonella typhimurium), to control antigens, such as Candida albicans, mumps virus and purified protein derivative, to the recombinant mycobacterial 65-kDa heat-shock protein (hsp 65) and the mitogen phytohemagglutinin (PHA) in 16 patients with different inflammatory rheumatic diseases. T…

Salmonella typhimuriumMicrobiology (medical)Antigens FungalT cellImmunologyArthritisInflammationBiologyLymphocyte ActivationPeripheral blood mononuclear cellEpitopeImmune systemEnterobacteriaceaeAntigenCandida albicansSynovial FluidmedicineHumansImmunology and AllergySynovial fluidPhytohemagglutininsHeat-Shock ProteinsYersinia enterocoliticaAntigens BacterialArthritisGeneral Medicinemedicine.diseasemedicine.anatomical_structureImmunologyLeukocytes Mononuclearmedicine.symptomMedical Microbiology and Immunology
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Lack of correlation between apoptosis and DNA single-strand breaks in X-irradiated human peripheral blood mononuclear cells in the course of ageing

1998

The dependence on age of both the basal and the X-radiation-induced levels of apoptosis was examined in human peripheral blood mononuclear cells (PBMC). In the same samples, the base value and the extent of induced DNA single-strand breaks were determined, using a sensitive and fast microplate assay. PBMC were isolated from blood of donors of various age groups (20-30, 40-60 and > 70 years of age) and X-irradiated ex vivo using a 6 MV linear accelerator to give a total exposure of 4 Gy. The mean basal levels of apoptosis in PBMC from donors in the 40-60 year age group and the > 70 year age group were found to be only slightly higher (by 20-10%) compared to that of the 20-30 year age group, …

SenescenceAgingProgrammed cell deathDNA damageX-RaysDNA Single-StrandedApoptosisBiologyPeripheral blood mononuclear cellAndrologyBlood cellmedicine.anatomical_structureApoptosisAgeingImmunologyLeukocytes MononuclearmedicineHumansRadiation-induced apoptosis; T-cells; Intrinsic radiosensitivity; Ionizing-radiation; Cervical-carcinoma; Human-lymphocytes; Immune-systemEx vivoDevelopmental BiologyMechanisms of Ageing and Development
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Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review

2021

Background: The study of hematic concentrations of alpha1 antitrypsin (AAT) is currently one step in the diagnosis of AAT deficiency. To try to clarify the relevance of the laboratory techniques, we carried out a systematic review of the literature. Methods: Studies evaluating the quantification of AAT in peripheral blood were searched in PubMed in July 2021. The selection criteria included (1) any type of study design that included a quantification of AAT in peripheral blood; (2) studies written in English or Spanish; (3) studies evaluating human beings; and (4) studies involving adults. Results: Out of 207 studies, the most frequently used techniques were nephelometry (43.9%), followed by…

Serummedicine.medical_specialtycongenital hereditary and neonatal diseases and abnormalitiesAAT deficiencyAlpha1-antitripsinReviewturbidimetryCommercial kitBlood concentrationPlasmanephelometryBlood concentrationInternal medicinemedicineplasmablood concentrationalpha1-antitripsinbusiness.industryRGeneral MedicinePeripheral bloodMedicinebusinessNephelometryserumNephelometryTurbidimetryJournal of Clinical Medicine
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Metilazione del DNA in artrite reumatoide

2005

Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…

Settore BIO/18 - Geneticainstead chromosomes of controls were almost uniformly decorated by brilliant grains. Studies on methylation of PTHrP gene promoter 2 performed on five CpG island internal sites using the Methylation-Sensitive Restriction Endonuclease Multiplex (MSREM)-PCR showed that one of the sites nearest the trascription starting point is heavy methylated in a significantly high number of RA patients. Thus RA seems to be characterized by genomewide hypomethylation associated with local hypermethylation like the most part of tumors. This result raises the possibility that susceptibility to lymphomas is related to abnormal DNA methylation levels and suggests the opportunity to evaluate the DNA methylation status in RA patientin fact the demethylating therapies together with diet and life style can act towards an increase of tumor risk. Future studies using a larger number of subjects could confirm these findings.Rheumatoid Arthritis (RA) is a chronic multisystem inflammatory disease characterized by high recurrence of lymphomas as well as hypercalcemia due to PTHrP overexpression. Because of DNA methylation plays a critical role in development of neoplasias we determined in RA patients the global DNA methylation status and local methylation pattern of the CpG island of one of the three promoters of PTHrP gene utilizing molecular and cytogenetic techniques. Investigations performed on DNA from peripheral blood of patients and donors amplified by Methylation-Sensitive Arbitrarily Primed (MeS-AP)-PCR indicated that RA is strongly associated with global DNA hypomethylation. Similarly chromosomal DNA methylation pattern analysis by indirect immunofluorescence technique with anti 5-methylcitosine antibody showed all peripheral lymphocyte metaphases from RA patients with chromosomes weakly fluorescent without discrete grain
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Cutaneous Exposure to the Superantigen Staphylococcal Enterotoxin B Elicits a T-Cell-Dependent Inflammatory Response

1996

We analyzed the impact of superantigens secreted by skin-colonizing Staphylococci on the skin and the associated lymphoid tissue following epicutaneous application and intracutaneous injection of small amounts of staphylococcal enterotoxin B (SEB). A single intracutaneous injection of 50 ng of SEB elicited a strong inflammatory response in the skin of BALB/c mice. Three to 6 h later, we observed langerhans cell activation, mast cell degranulation, vasodilation, upregulation of ICAM-1, and induction of VCAM-1 on dermal blood vessels, with vascular adhesion of granulocytes. by 12 to 24 h, cell infiltration of the dermis increased, reaching the epidermis. Among the infiltrating leukocytes, a s…

Staphylococcus aureusLangerhans cellT cellVascular Cell Adhesion Molecule-1InflammationDermatitischemical and pharmacologic phenomenaDermatologyCD8-Positive T-LymphocytesPeripheral blood mononuclear cellBiochemistryEnterotoxinsMicemedicineSuperantigenAnimalsIntradermal injectionMolecular BiologyMice Inbred BALB CSuperantigensbusiness.industryDegranulationhemic and immune systemsCell Biologybiological factorsmedicine.anatomical_structureImmunologyTumor necrosis factor alphaFemalemedicine.symptombusinessJournal of Investigative Dermatology
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Type-I interferons are potent inhibitors of interleukin-8 production in hematopoietic and bone marrow stromal cells

1993

Abstract nterleukin-8 (IL-8) is produced by many cell types upon stimulation with bacterial products or inflammation-associated cytokines such as tumor necrosis factor-alpha and IL-1. Interferons (IFNs) represent another group of cytokines that are induced by similar stimuli in inflammatory reactions. We show now that type-I IFNs are potent inhibitors of IL-8 expression in vitro and in vivo. A significant reduction of both secretion of IL-8 protein and accumulation of IL-8 mRNA in vitro was observed in several cell types comprising peripheral blood mononuclear cells (PBMNC) from healthy donors and from patients with chronic myelogenous leukemia (CML), the myelomonocytic cell line THP-1, and…

Stromal cellmedicine.medical_treatmentImmunologyCell BiologyHematologyBiologymedicine.diseaseBiochemistryPeripheral blood mononuclear cellHaematopoiesisLeukemiaCytokinemedicine.anatomical_structureInterferonImmunologymedicineCancer researchBone marrowChronic myelogenous leukemiamedicine.drugBlood
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Quantitation of antigen-reactive T cells in peripheral blood by IFNgamma-ELISPOT assay and chromium-release assay: a four-centre comparative trial

2000

The ELISPOT assay is increasingly being used for the monitoring of the induction of antigen-reactive T cells in cancer vaccination trials. In order to evaluate the reliability of T cell frequency analysis with the ELISPOT assay, a comparative study was performed in four European laboratories. Six samples from healthy subjects were analyzed for the frequency of influenza-reactive CD8+ T cells in peripheral blood mononuclear cells (PBMC) by IFNgamma-ELISPOT assay. In addition, one laboratory determined cytotoxic T cell precursor (CTL) frequencies in these samples by limiting dilution chromium-release assay (LDA), and three laboratories performed a variant of the LDA, the multiple microculture…

T cellImmunologyEpitopes T-LymphocyteIndicator Dilution TechniquesEnzyme-Linked Immunosorbent AssayCD8-Positive T-LymphocytesLymphocyte ActivationPeripheral blood mononuclear cellViral Matrix ProteinsInterferon-gammaAntigenHLA-A2 AntigenHumansImmunology and AllergyCytotoxic T cellMedicineAntigens ViralImmunodominant Epitopesbusiness.industryELISPOTMolecular biologyChromium RadioisotopesHIV Reverse TranscriptasePeptide FragmentsCTL*medicine.anatomical_structureImmunologyLeukocytes MononuclearCancer vaccinebusinessCD8T-Lymphocytes Cytotoxic
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HLA-DR phenotypes and blood levels of T cell subsets

1984

Blood mononuclear cell and T cell subsets values were analyzed in 53 Sicilian individuals according to HLA-DR phenotypes. The results demonstrate that DR1-positive subjects show a significant increase of blood T cell subsets whereas DR3-positive subjects show a non-significant decrease of these values. These results suggest that gene(s) associated with HLA-DR could be one of the factors which affect blood levels of T cell subsets.

T-LymphocytesT cellImmunologyHLA-DR1 AntigenHistocompatibility Antigens Class IIHLA-DR AntigensGeneral MedicineImmunogeneticsT lymphocyteBiologyBiochemistryPhenotypePeripheral blood mononuclear cellLeukocyte CountHLA-DR3 AntigenPhenotypemedicine.anatomical_structureImmunologyGeneticsmedicineHLA-DRHumansImmunology and AllergyGeneTissue Antigens
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Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence

2014

Objective: To analyze Mucograft®(MG), a recently introduced collagen matrix, in vitro and in vivo, and compare it with BioGide®(BG), a well-established collagen membrane, as control. Material and Methods: A detailed analysis of the materials surface and ultra-structure was performed. Cellular growth patterns and proliferation rates of human fibroblasts on MG and BG were analyzed in vitro. In addition, the early tissue reaction of CD-1 mouse to these materials was analyzed by means of histological and histomorphometrical analysis. Results: MG showed a three-fold higher thickness both in dry and wet conditions, when compared to BG. The spongy surface of BG significantly differed from that of …

Time FactorsCell SurvivalSurface PropertiesCellBiocompatible MaterialsMatrix (biology)BiologyPeripheral blood mononuclear cellCollagen Type IIIMiceRandom AllocationCollagen type IIn vivoMaterials TestingmedicineAnimalsHumansddc:610Bone regenerationGeneral DentistryCells CulturedCell ProliferationGuided tissue regenerationCell growthReproducibility of ResultsAnatomyOriginal ArticlesFibroblastsCollagen type IIIMolecular biologyImmunohistochemistryIn vitroBone regenerationlcsh:RK1-715medicine.anatomical_structurelcsh:DentistryFemaleCollagenJournal of Applied Oral Science
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