6533b820fe1ef96bd1279a39
RESEARCH PRODUCT
Lack of correlation between apoptosis and DNA single-strand breaks in X-irradiated human peripheral blood mononuclear cells in the course of ageing
Evelyn HeidenreichHanoch SlorChristian ChauvinJoachim KutznerHeinz C. SchröderRenato BatelKristina Elmendorff-dreikornsubject
SenescenceAgingProgrammed cell deathDNA damageX-RaysDNA Single-StrandedApoptosisBiologyPeripheral blood mononuclear cellAndrologyBlood cellmedicine.anatomical_structureApoptosisAgeingImmunologyLeukocytes MononuclearmedicineHumansRadiation-induced apoptosis; T-cells; Intrinsic radiosensitivity; Ionizing-radiation; Cervical-carcinoma; Human-lymphocytes; Immune-systemEx vivoDevelopmental Biologydescription
The dependence on age of both the basal and the X-radiation-induced levels of apoptosis was examined in human peripheral blood mononuclear cells (PBMC). In the same samples, the base value and the extent of induced DNA single-strand breaks were determined, using a sensitive and fast microplate assay. PBMC were isolated from blood of donors of various age groups (20-30, 40-60 and > 70 years of age) and X-irradiated ex vivo using a 6 MV linear accelerator to give a total exposure of 4 Gy. The mean basal levels of apoptosis in PBMC from donors in the 40-60 year age group and the > 70 year age group were found to be only slightly higher (by 20-10%) compared to that of the 20-30 year age group, whereas the extent of DNA damage strongly and significantly (P 70), compared to age group 20-30. The results show that X-ray-induced apoptosis and DNA damage in PBMC are not correlated during ageing.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-12-01 | Mechanisms of Ageing and Development |