Search results for "phosphate"

Ultra-high-pressure liquid chromatography tandem mass spectrometry method for the determination of 9 organophosphate flame retardants in water samples

2016

Few methods are available for comprehensive organophosphate flame retardants (PFRs) detection in water and wastewater. Gas chromatography has been employed previously, but this approach is less selective, not amenable for use with deuterated standards and can suffer unfavorable fragmentation. Ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-QqQ-MS/MS) has become the most promising platform, already applied successfully for analysis of selected PFRs in some environmental matrices like water and wastewater. However, the presence of some interferences from the dissolvent, the equipment and the used materials should be taken into account. The procedure involves: The fir…

Determination of organic and inorganic anions in insect haemolymph and midgut contents by ion chromatography

1997

A simple and rapid ion-exchange chromatographic method with conductimetric detection for the determination of carboxylic acids (succinic, malic and citric) and inorganic anions (chloride, phosphate and sulphate) in haemolymph and midgut content of Lepidoptera larvae is provided. The mobile phase is 0.975 mM phthalic acid at pH 4.1. The procedure of sample extractions was simple. Comparison of the results with whose reported in the bibliography showed that the values were similar. The ion-exchange chromatographic–conductimetric detection method permits the analysis of various organic and inorganic anions in small biological samples.

Extraktionschromatographische trennung der freien porphyrine auf tri-n-butylphosphat-s?ulen im pH-gradienten

1969

Abstract The method described allows the separation of the free porphyrins on a tri-n-butyl phosphate (TBP) column by elution with a pH gradient. The prophyrins are eluted from the column at discrete pH values (xD) according to the number of carboxyl groups in the side chains. Separation of isomers could not be observed under the conditions used. The xD values indicate the centre of the pH-interval, in which the dissociation of the carboxyl groups occurs. This finding could be confirmed by spectro-photometric measurements. By combining the method proposed with the method of separation by extraction chromatography in the TBP/IN HCl partition system and the thin-layer technique in the 2,6-lut…

Determination of alternative and conventional chelating agents as copper(II) complexes by capillary zone electrophoresis—The first use of didecyldime…

2006

Abstract A capillary zone electrophoresis (CZE) method for analyzing 11 chelating agents [β-alaninediacetic acid (β-ADA), trans-1,2-diaminocyclohexane- N , N , N ′, N ′-tetraacetic acid (CDTA), diethylenetriaminepentaacetic acid (DTPA), ethylenediaminetetraacetic acid (EDTA), N -(2-hydroxyethyl)ethylenediamine- N , N ′, N ′-triacetic acid (HEDTA), N -(2-hydroxyethyl)iminodiacetic acid (HEIDA), iminodiacetic acid (IDA), methylglycinediacetic acid (MGDA), nitrilotriacetic acid (NTA), 1,3-diaminopropane- N , N , N ′, N ′-tetraacetic acid (PDTA) and triethylenetetraaminehexaacetic acid (TTHA)] as negatively charged copper(II) complexes has been established. Both conventional and alternative che…

ChemInform Abstract: K3Cr2(PS4)3: A New Chromium Thiophosphate with a One-Dimensional [Cr2(PS4)3]3- Anion Chain.

2010

Cloning, purification, and nucleotide-binding traits of the catalytic subunit A of the V1VO ATPase from Aedes albopictus.

2007

The Asian tiger mosquito, Aedes albopictus, is commonly infected by the gregarine parasite Ascogregarina taiwanensis, which develops extracellularly in the midgut of infected larvae. The intracellular trophozoites are usually confined within a parasitophorous vacuole, whose acidification is generated and controlled by the V(1)V(O) ATPase. This proton pump is driven by ATP hydrolysis, catalyzed inside the major subunit A. The subunit A encoding gene of the Aedes albopictus V(1)V(O) ATPase was cloned in pET9d1-His(3) and the recombinant protein, expressed in the Escherichia coli Rosetta 2 (DE3) strain, purified by immobilized metal affinity- and ion-exchange chromatography. The purified prote…

Modulation of geraniol metabolism during alcohol fermentation

2004

J. Inst. Brew. 110(3), 213–219, 2004 Geraniol is the main monoterpenol present in Gewurtztraminer white grapes and in some red aromatic grapes from northern Italy. During fermentation, the geraniol concentration showed a dramatic drop and a small amount was transformed into linalool and -terpineol by an acid catalysed chemical reaction. Yeast are responsible for changing most of the geraniol to citronellol through enzymatic reactions. The final aroma of wine is due mainly to both the untransformed geraniol and the formed citronellol. In this study, competition between the geraniol-citronellol transformation and another metabolic process, in which according to our hypothesis sterol biosynthe…

Physiological and molecular analysis of the maturation process in fruits of Clementine Mandarin and one of its late-ripening mutants

2009

Peel color is one of the main features affecting citrus quality. Clementine is a widespread citrus species with several mutants showing a delay in pigmentation and harvesting. This work characterizes the fruit development and ripening of two clementine clones, 'Comune', a widespread variety, and one of its natural mutations, 'Tardivo', which differ by a delayed color-break and extended harvest period. Morphological, chemical, and molecular analyses were carried out on fruits of both genotypes during the whole maturation process. Analysis showed that mutation did not affect ripening characteristics such as juice acidity and TSS. However, biochemical and molecular analysis revealed marked dif…

Collagen-induced differential expression of an RNA polymerase subunit by breast cancer cells

2005

It was previously reported that the stroma of ductal infiltrating carcinoma (DIC) of the human breast contains considerable amount of an embryo-foetal collagen type, OF/LB (onco-foetal/laminin-binding), and that adhesion of 8701-BC DIC cells onto OF/LB collagen substrates selectively promotes cell growth, motility, production of extracellular lytic enzymes and invasion "in vitro" if compared with other collagen species. To detect possible transcriptional differences for regulatory proteins following OF/LB collagen-cell interactions, we submitted RNA preparations from 8701-BC cells grown on collagen type I, IV and OF/LB to "differential display"-PCR in the presence of degenerate C(2)H(2) zin…

Novel Biodegradable Composite of Calcium Phosphate Cement and the Collagen I Mimetic P-15 for Pedicle Screw Augmentation in Osteoporotic Bone

2021

Osteoporotic vertebral fractures often necessitate fusion surgery, with high rates of implant failure. We present a novel bioactive composite of calcium phosphate cement (CPC) and the collagen I mimetic P-15 for pedicle screw augmentation in osteoporotic bone. Methods involved expression analysis of osteogenesis-related genes during osteoblastic differentiation by RT-PCR and immunostaining of osteopontin and Ca2+ deposits. Untreated and decalcified sheep vertebrae were utilized for linear pullout testing of pedicle screws. Bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DEXA). Expression of ALPI II (p &lt