Search results for "polyQ"
showing 7 items of 7 documents
The importance of definitions in the study of polyQ regions: A tale of thresholds, impurities and sequence context
2020
Graphical abstract
Glutamine Codon Usage and polyQ Evolution in Primates Depend on the Q Stretch Length
2018
Abstract Amino acid usage in a proteome depends mostly on its taxonomy, as it does the codon usage in transcriptomes. Here, we explore the level of variation in the codon usage of a specific amino acid, glutamine, in relation to the number of consecutive glutamine residues. We show that CAG triplets are consistently more abundant in short glutamine homorepeats (polyQ, four to eight residues) than in shorter glutamine stretches (one to three residues), leading to the evolutionary growth of the repeat region in a CAG-dependent manner. The length of orthologous polyQ regions is mostly stable in primates, particularly the short ones. Interestingly, given a short polyQ the CAG usage is higher in…
Nuclear inclusions of pathogenic ataxin-1 induce oxidative stress and perturb the protein synthesis machinery
2020
Spinocerebellar ataxia type-1 (SCA1) is caused by an abnormally expanded polyglutamine (polyQ) tract in ataxin-1. These expansions are responsible for protein misfolding and self-assembly into intranuclear inclusion bodies (IIBs) that are somehow linked to neuronal death. However, owing to lack of a suitable cellular model, the downstream consequences of IIB formation are yet to be resolved. Here, we describe a nuclear protein aggregation model of pathogenic human ataxin-1 and characterize IIB effects. Using an inducible Sleeping Beauty transposon system, we overexpressed the ATXN1(Q82) gene in human mesenchymal stem cells that are resistant to the early cytotoxic effects caused by the expr…
A new synthetic entry into the tricyclo[3.3.0.03,7] octane skeleton
1987
Abstract A short synthesis of dimethyl tricyclo[3.3.0.03,7] octane-1,5-dicarboxylate, 13 , and its 3,7-dimethyl-derivative, 14 , by iodine oxidation of the bis-enolate derived from the corresponding dimethyl cis -bicyclo[3.3.0] octane-3,7-dicarboxylate, 11 or 12 , is described.
Synergistic activation of AMPK prevents from polyglutamine-inducedtoxicity inCaenorhabditis elegans
2020
11 páginas, 4 figuras. Supplementary material related to this article can be found, in the online version, at doi: https://doi.org/10.1016/j.phrs.2020.105105.
Dynamics of a Protein Interaction Network Associated to the Aggregation of polyQ-Expanded Ataxin-1
2020
Background: Several experimental models of polyglutamine (polyQ) diseases have been previously developed that are useful for studying disease progression in the primarily affected central nervous system. However, there is a missing link between cellular and animal models that would indicate the molecular defects occurring in neurons and are responsible for the disease phenotype in vivo. Methods: Here, we used a computational approach to identify dysregulated pathways shared by an in vitro and an in vivo model of ATXN1(Q82) protein aggregation, the mutant protein that causes the neurodegenerative polyQ disease spinocerebellar ataxia type-1 (SCA1). Results: A set of common dysregulated pathwa…
Dažāda veida mākslīgo asaru ietekme uz sausās acs radītajām pazīmēm un simptomiem
2021
Maģistra darbs ir uzrakstīts angļu valodā uz 5 5 lappaspusēm. Tas satur 13 attēlus, 19 tabulas un 30 atsauces uz literatūras avotiem. Šī pētījuma mērķis ir novērtēt dažādu mākslīgo asaru ietekmi uz sausu acu simptomiem un pazīmēm. Sauso acu slimība (DED) ir hroniska, daudzfaktoru acu virsmas slimība, kas noved pie diskomforta, redzes traucējumiem un asaru plēvītes nestabilitātes, ar iespējamu acu virsmas bojājumu. Vecums un dzimums tiek mi nēts par svarīgākajiem faktoriem, kas izraisa sauso acu slimību, turpretī citi riski bieži netiek ņemti vērā. Lai nozīmētu DED ārstēšanu, jāveic dažādi diagnostikas testi. Mūsdienās ir pieejamas atšķirīgas ārstēšanas metodes, bet mākslīgās asaras ir visbi…