Search results for "printing"

showing 10 items of 532 documents

Magnetic molecularly imprinted polymers for the selective determination of cocaine by ion mobility spectrometry

2018

Abstract Magnetic molecularly imprinted polymers (MMIPs) were prepared for cocaine recognition by bulk polymerization in the presence of magnetic nanoparticles (MNPs). Two reagents (polyethylene glycol (PEG) and 3-(trimethoxysilyl)propyl methacrylate (V)) were used for MNPs modification. MMIPs were characterized and compared in terms of loading capacity, reusability, accuracy and precision for the extraction of cocaine from saliva samples. It was observed that V-MMIPs gave higher physical stability than PEG-MMIPs. Thus, V-MMIP were used for the analysis of cocaine users saliva. The developed procedure based on ion mobility spectrometry (IMS) provided limits of detection and quantification o…

AdultMaleBulk polymerizationPolymersIon-mobility spectrometry02 engineering and technologyPolyethylene glycolTandem mass spectrometryMethacrylate01 natural sciencesBiochemistryPolyethylene GlycolsAnalytical ChemistryMolecular ImprintingMagneticsYoung Adultchemistry.chemical_compoundCocaineIon Mobility SpectrometryHumansOrganosilicon CompoundsSalivaChromatography High Pressure LiquidDetection limitChromatography010401 analytical chemistryOrganic ChemistryMolecularly imprinted polymerSignal Processing Computer-AssistedGeneral Medicine021001 nanoscience & nanotechnology0104 chemical scienceschemistryMethacrylatesMagnetic nanoparticlesFemale0210 nano-technologyJournal of Chromatography A
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Virtual Planning and 3D printing modeling for mandibular reconstruction with fibula free flap

2017

Background This study was to evaluate the use of virtual planning and 3D printing modeling in mandibular reconstruction and compare the operation time and surgical outcome of this technique with conventional method. Material and Methods Between 2014 and 2017, 15 patients underwent vascularized fibula flap mandibular reconstruction using virtual planning and 3D printing modeling. Titanium plates were pre-bent using the models and cutting guides were used for osteotomies. 15 patients who underwent mandibular reconstruction using fibula flap without aid of virtual planning and 3D printing models were selected as control group. The operation time was recorded and compared in two groups. Accurac…

AdultMaleComputer science3D printingFree flapFree Tissue FlapsPatient Care Planning03 medical and health sciencesYoung Adult0302 clinical medicineOperation timeHumansFibulaMandibular reconstructionGeneral DentistryRetrospective StudiesOrthodonticsbusiness.industryResearchMandible030206 dentistryFibula flapMiddle Aged:CIENCIAS MÉDICAS [UNESCO]OtorhinolaryngologyVirtual planningFibula030220 oncology & carcinogenesisPrinting Three-DimensionalUNESCO::CIENCIAS MÉDICASSurgeryFemaleMandibular ReconstructionOral Surgerybusiness
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Where is the locus of the lowercase advantage during sentence reading?

2016

While most models of visual word identification and reading posit that a word's visual codes are rapidly transformed onto case-invariant representations (i.e., table and TABLE would equally activate the word unit corresponding to "table"), a number of experiments have shown a lowercase advantage in various word identification and reading tasks. In the present experiment, we examined the locus of this lowercase advantage by comparing the pattern of eye movements when reading sentences in lowercase vs. uppercase. Each sentence contained a target word that was high or low in word-frequency. Overall, results showed faster reading times for lowercase than for uppercase sentences. More important,…

AdultMaleLetter caseEye MovementsComputer scienceSpeech recognitionExperimental and Cognitive PsychologyFixation Ocular050105 experimental psychology03 medical and health sciences0302 clinical medicineArts and Humanities (miscellaneous)Developmental and Educational PsychologyHumansSentence reading0501 psychology and cognitive sciencesVisual WordCommunicationbusiness.industry05 social sciencesEye movementGeneral MedicineGazeSemanticsWord lists by frequencyReadingWord identificationPrintingFemaleComprehensionbusiness030217 neurology & neurosurgerySentenceActa Psychologica
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Application of mtDNA sequence analysis in forensic casework for the identification of human remains

2000

Abstract In four forensic cases of unidentified skeletal remains investigated in the last year, we were able to attach three to missing persons. In one case we could show that the discovered bone sample did not fit to a missing child. The method for mitochondrial DNA analysis for the routine identification of skeletal remains was established in our institute by typing bone samples of defined age obtained from Frankfurt's cemetery. Reproducible results were obtained for bones up to 75 years old. For analysis the bone samples were pulverised to fine powder, decalcified and DNA was extracted. From the DNA we amplified a 404-bp fragment from HV-1 and a 379-bp fragment from HV-2 of the mtDNA con…

AdultMaleMitochondrial DNASequence analysisMinisatellite RepeatsBiologyDNA MitochondrialPolymerase Chain ReactionBone and BonesPathology and Forensic Medicinelaw.inventionlawAge Determination by SkeletonHumansChildPolymerase chain reactionGeneticsmtDNA control regionForensic anthropologySequence Analysis DNADNA FingerprintingHypervariable regionForensic identificationDNA profilingForensic AnthropologyFemaleLawForensic Science International
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Clonal evolution in relapsed NPM1-mutated acute myeloid leukemia.

2013

Mutations in the nucleophosmin 1 (NPM1) gene are considered a founder event in the pathogenesis of acute myeloid leukemia (AML). To address the role of clonal evolution in relapsed NPM1-mutated (NPM1mut) AML, we applied high-resolution, genome-wide, single-nucleotide polymorphism array profiling to detect copy number alterations (CNAs) and uniparental disomies (UPDs) and performed comprehensive gene mutation screening in 53 paired bone marrow/peripheral blood samples obtained at diagnosis and relapse. At diagnosis, 15 aberrations (CNAs, n = 10; UPDs, n = 5) were identified in 13 patients (25%), whereas at relapse, 56 genomic alterations (CNAs, n = 46; UPDs, n = 10) were detected in 29 patie…

AdultMaleNPM1MyeloidImmunologyBiologyGene mutationBiochemistrySomatic evolution in cancerPolymorphism Single NucleotideDNA Methyltransferase 3AClonal EvolutionYoung AdultRecurrenceRisk FactorsmedicineHumansDNA (Cytosine-5-)-MethyltransferasesAgedChromosomes Human Pair 13Myeloid leukemiaNuclear ProteinsCell BiologyHematologyMiddle Agedmedicine.diseasePrognosisMinimal residual diseaseDNA FingerprintingLeukemiaETV6Leukemia Myeloid Acutemedicine.anatomical_structureCancer researchFemaleChromosomes Human Pair 9NucleophosminGene DeletionBlood
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Whole body frontal plane mechanics across walking, running, and sprinting in young and older adults

2016

This study investigated the whole body frontal plane mechanics among young (26 ± 6 years), early old (61 ± 5 years), and old (78 ± 4 years) adults during walking, running, and sprinting. The age-groups had similar walking (1.6 m/s) and running (4.0 m/s) speeds, but different maximal sprinting speed (young 9.3 m/s, early old 7.9 m/s, and old 6.6 m/s). Surprisingly, although the old group exerted much lower vertical ground reaction force during running and sprinting, the hip frontal plane moment did not differ between the age-groups. Kinematic analysis demonstrated increased hip adduction and pelvis drop, as well as reduced trunk lateral flexion among old adults, especially during sprinting. …

AdultMalePhysical Therapy Sports Therapy and RehabilitationKinematicsgaitPelvisjuoksuYoung Adultwalking03 medical and health sciencesSpatio-Temporal Analysis0302 clinical medicinerunningmedicineHumansOrthopedics and Sports MedicineRange of Motion ArticularMuscle Skeletalfrontal plane mechanicsPelvisAgedBalance (ability)Aged 80 and overbusiness.industryagingTorsota3141ta3142030229 sport sciencesMechanicsMiddle AgedTorsoTrunkGaitBiomechanical Phenomenakävelyikääntyminenmedicine.anatomical_structuresprintingkinematicskineticsCoronal planeHip JointAdductor musclesbusiness030217 neurology & neurosurgeryScandinavian Journal of Medicine & Science in Sports
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3D-printing-assisted fabrication of chitosan scaffolds from different sources and cross-linkers for dental tissue engineering

2021

The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering. Chitosan-based scaffolds were prepared by the emulsion freeze-drying technique. Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure. Chemical composition and cross-linking were evaluated by Fourier transform infrared-attenuated total reflectance spectroscopy. Compression tests were performed to evaluate scaffold mechanical properties. S…

AdultMaleScaffoldMaterials scienceRD1-811AdolescentCompressive StrengthScanning electron microscopeSimulated body fluid0206 medical engineeringBiocompatible MaterialsDiseases of the musculoskeletal system02 engineering and technologyApatiteChitosanYoung Adultchemistry.chemical_compoundTissue engineeringfungal chitosanDental pulp stem cellsMaterials TestingHumansIridoidsCells CulturedDental PulpChitosanTissue EngineeringTissue ScaffoldsStem Cellstechnology industry and agricultureSilanes020601 biomedical engineeringdental pulp stem cellsRC925-935chemistryvisual_artPrinting Three-Dimensionalvisual_art.visual_art_mediumGenipinSurgeryFemalePorosityBiomedical engineeringEuropean Cells and Materials
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BYOTYPES AND RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) PROFILES OF SUBGINGIVAL CANDIDA ALBICANS ISOLATES IN HIV INFECTION

2005

ABSTRACT: A group of subgingival isolates of C. albicans recovered from Italian HIV-positive (HIV+) subjects were characterized both phenotypically and genotypically. Phenotyping of the isolates was carried out by a biotyping method based on the enzyme profiles, carbohydrate assimilation patterns and boric acid resistance of the yeasts. Genotyping was performed through randomly amplified polymorphic DNA (RAPD) analysis. Five biotypes were found among the 29 subgingival C. albicans strains examined. The predominant biotypes were A1R (55.17%), A1S (24.14%), and A2R (13.79%), while the biotypes A11R and A13R were represented by a single isolate each. RAPD profiles identified 15 genotypes among…

AdultMaleSettore MED/07 - Microbiologia E Microbiologia ClinicaAntifungal AgentsAIDS-Related Opportunistic InfectionsGenotypeSettore MED/42 - Igiene Generale E ApplicataDNA FingerprintingRandom Amplified Polymorphic DNA TechniquePhenotypeCandida albicans HIV-positiveBoric AcidsItalySettore MED/28 - Malattie OdontostomatologicheCandidiasis OralDrug Resistance FungalCandida albicansGingival DiseasesHumansFemaleDNA FungalMycological Typing Techniques
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Epimutation at human chromosome 14q32.2 in a boy with a upd(14)mat-like clinical phenotype.

2009

Recently, three reports described deletions and epimutations affecting the imprinted region at chromosome 14q32.2 in individuals with a phenotype typical for maternal uniparental disomy of chromosome 14 [upd(14)mat]. In this study, we describe another patient with upd(14)mat-like phenotype including low birth weight, neonatal feeding problems, muscular hypotonia, motor and developmental delay, small hands and feet, and truncal obesity. Conventional cytogenetic analyses, fluorescence in situ hybridization subtelomere screening, multiplex ligation-dependent probe amplification analysis of common microdeletion and microduplication syndromes, and methylation analysis of SNRPN all gave normal re…

AdultMalecongenital hereditary and neonatal diseases and abnormalitiesMolecular Sequence DataMothersBiologyMethylationPolymorphism Single NucleotideEpigenesis GeneticGenomic ImprintingIntergenic regionGeneticsmedicineHumansAbnormalities MultipleEpigeneticsChildGenetics (clinical)GeneticsChromosomes Human Pair 14Muscular hypotoniamedicine.diagnostic_testBase SequenceChromosomeUniparental DisomySubtelomerePhenotypeDifferentially methylated regionsPhenotypeMutationFemaleFluorescence in situ hybridizationClinical genetics
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Effects of high doses of toluene on color vision.

1999

High exposure to toluene may cause optic neuropathy and retinopathy, both associated with dyschromatopsia. Another solvent, ethanol, is known to induce acute blue-yellow dyschromatopsia. This study investigated the acute effects of high doses of toluene on color vision. Eight male printshop workers were examined before and after cleaning printing containers with pure toluene. After cleaning, concentrations of toluene in blood were between 3.61 and 7.37 mg/l. Color vision was tested with the Farnsworth panel D-15 test, the Lanthony desaturated panel D-15 test, and the Standard Pseudoisochromatic Plates part 2. For control of possible acute effects, eight workers of a metal-working factory wi…

AdultMalemedicine.medical_specialtygenetic structuresColor visionToxicologyOptic neuropathyCellular and Molecular Neurosciencechemistry.chemical_compoundDevelopmental NeuroscienceOphthalmologyOccupational ExposureHigh dosesMedicineHumansDyschromatopsiaToluene toxicitybusiness.industrymedicine.diseaseTolueneSurgerychemistryAcute exposurePrintingbusinessColor PerceptionRetinopathyTolueneNeurotoxicology and teratology
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