Search results for "probe"

FACS-based protocol to assess cytotoxicity and clonogenic potential of colorectal cancer stem cells using a Wnt/β-catenin signaling pathway reporter

2021

Summary Cancer stem cells (CSCs) play a key role in tumor initiation and progression. A real-time tool to evaluate the activation of CSC-specific signaling pathways is crucial for the study of this cancer cell subset. Here, we present a protocol to monitor, in vitro, the activation of Wnt/β-catenin signaling pathway, which is considered a functional biomarker for colorectal CSCs (CR-CSCs). This flow-cytometry-based protocol allows it to isolate CR-CSCs and to evaluate their cytotoxicity upon anti-tumor treatments. For complete details on the use and execution of this protocol, please refer to Di Franco et al. (2021).

Quantum Probes for the Characterization of Nonlinear Media

2021

Active optical media leading to interaction Hamiltonians of the form H=λ˜(a+a†)ζ represent a crucial resource for quantum optical technology. In this paper, we address the characterization of those nonlinear media using quantum probes, as opposed to semiclassical ones. In particular, we investigate how squeezed probes may improve individual and joint estimation of the nonlinear coupling λ˜ and of the nonlinearity order ζ. Upon using tools from quantum estimation, we show that: (i) the two parameters are compatible, i.e., the may be jointly estimated without additional quantum noise

Characterization of single semiconductor nanowires by synchrotron radiation nanoprobe

2012

In this work, we report on the results of the characterization of single semiconductor nanowires by x-ray fluorescence nanoprobe. Wurtzite InGaN and Co-implanted ZnO single nanowires were studied. Ternary semiconductor nanowires show an axial inhomogeneous elemental distribution, with Ga accumulating at the bottom and In at the top of the wires. The ZnO NWs, on the other hand, show a homogeneous distribution of the Co implanted along the nanowires, without signatures of clustering or segregation effects induced by the implantation. No signatures of unintentional doping are observed neither in the InGaN nor the ZnO NWs. These overall results demonstrate the suitability of X-ray fluorescence …

Species-specific identification of Dekkera/Brettanomyces yeasts by fluorescently labeled DNA probes targeting the 26S rRNA.

2007

Sequencing of the complete 26S rRNA genes of all Dekkera/Brettanomyces species colonizing different beverages revealed the potential for a specific primer and probe design to support diagnostic PCR approaches and FISH. By analysis of the complete 26S rRNA genes of all five currently known Dekkera/Brettanomyces species (Dekkera bruxellensis, D. anomala, Brettanomyces custersianus, B. nanus and B. naardenensis), several regions with high nucleotide sequence variability yet distinct from the D1/D2 domains were identified. FISH species-specific probes targeting the 26S rRNA gene's most variable regions were designed. Accessibility of probe targets for hybridization was facilitated by the constr…

Monitoraggio in tempo reale dello stress idrico del pesco

2020

La frutticoltura odierna è sempre più caratterizzata dall’utilizzo di nuove tecnologie che si basano sull’utilizzo di molteplici sensori. Una delle grandi sfide della Smart Agriculture (agricoltura intelligente) è quella di integrare le informazioni provenienti da sensori remoti, prossimali e a contatto in modelli informativi in grado di sostenere gli agricoltori, in modo tale da determinare un sostanziale beneficio economico per le aziende. La complessità delle informazioni ottenute scoraggia l’integrazione di dati apparentemente molto diversi, ma allo stesso tempo apre le porte a nuovi modelli d’impresa. In questo lavoro vengono messi in risalto alcuni vantaggi e difficoltà dell’utilizzo …

Interspersed telomeric sequences (TTAGGG)n distribution in Primates and Tupaia minor (Scandentia)

2017

Towards water operations of the future.

2018

Nowadays multiple use of “smart” word is provided in many fields including research. Sometimes it is a synonym for chic, elegant or straight. A smart water system is not the same as an elegant, glamorous or fashionable system. Instead it operates to fulfil the requirements of producing an acceptable product while keeping the energy and resource requirements at a minimum. However, while cost savings has up until recently been our primary aim of making water and wastewater operations more efficient, there is now a more pressing agenda of sustainability driving the smart agenda. The primary aim of developing our utilities into a higher level of smart is hence moving from a singular focus on co…

Deletion of NSD1 exon 14 in Sotos syndrome: first description.

2010

Closure to “Laboratory and Field Calibration of the Diviner 2000 Probe in Two Types of Soil” by J. Haberland, R. Gálvez, C. Kremer, and C. Carter

2015

The authors deal with the quite interesting and actual problem of Diviner 2000 capacitance probe calibration and present some field and laboratory data obtained on two different layers (0–0.26 and 0.26–0.50 cm) of the same soil profile, characterized by different textural class. The importance of site-specific calibration of sensors used to monitor soil or plant water status assumes a particular relevance in semiarid environments where the application of precision irrigation represents an appropriate management strategy aimed to achieve high values of water use efficiency (Cammalleri et al. 2013). Moreover, in clay soils, physical properties are strongly influenced by soil water content (Pr…

Assessing Field and Laboratory Calibration Protocols for the Diviner 2000 Probe in a Range of Soils with Different Textures

2016

Frequency domain reflectometry (FDR) downhole sensors have been increasingly used for soil moisture field monitoring because they allow measurement, even continuously, along a soil profile. Moreover, they can also be installed with minimal soil disturbance around the access tube. The objectives of the paper were to assess the field and laboratory calibration protocols for a FDR capacitance probe (Diviner 2000) for a range of soils characterized by different particle size distributions and shrink/swell potential and to propose a practical and effective protocol on the basis of undisturbed soil samples, accounting for soil shrinkage/swelling processes characterizing swelling clay soils. The e…