Search results for "pyrrolomycin"

showing 9 items of 9 documents

New Synthetic Nitro-Pyrrolomycins as Promising Antibacterial and Anticancer Agents

2020

: Pyrrolomycins (PMs) are polyhalogenated antibiotics known as powerful biologically active compounds, yet featuring high cytotoxicity. The present study reports the antibacterial and antitumoral properties of new chemically synthesized PMs, where the three positions of the pyrrolic nucleus were replaced by nitro groups, aiming to reduce their cytotoxicity while maintaining or even enhancing the biological activity. Indeed, the presence of the nitro substituent in diverse positions of the pyrrole determined an improvement of the minimal bactericidal concentration (MBC) against Gram-positive (i.e., Staphylococcus aureus) or -negative (i.e., Pseudomonas aeruginosa) pathogen strains as compare…

0301 basic medicineMicrobiology (medical)Staphylococcus aureusmedicine.drug_classAntibioticspyrrolomycinmedicine.disease_causeSettore BIO/19 - Microbiologia Generale01 natural sciencesBiochemistryMicrobiologypyrrolic nucleusHCT116Article03 medical and health sciencesantibacterial activityMCF 7medicinePharmacology (medical)General Pharmacology Toxicology and PharmaceuticsSettore BIO/06 - Anatomia Comparata E CitologiaCytotoxicityheterocyclesMinimum bactericidal concentrationantitumoral activity010405 organic chemistryChemistryPseudomonas aeruginosalcsh:RM1-950MCF7Biological activitySettore CHIM/08 - Chimica Farmaceutica0104 chemical scienceslcsh:Therapeutics. Pharmacology030104 developmental biologyInfectious DiseasesMCF-7BiochemistryStaphylococcus aureusPseudomonas aeruginosaNitroAntibiotics
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In vitro anti-Gram-positive and anti-staphylococcal biofilm activity of newly halogenated pyrroles related to Pyrrolomycins

2007

Microbiology (medical)ChemistryStaphylococcusBiofilmGeneral MedicineMicrobial Sensitivity Testshalogenated pyrroleGram-Positive Bacteriaantistaphylococcal biofilm activityIn vitroMicrobiologyAnti-Bacterial AgentsStructure-Activity RelationshipInfectious DiseasesVancomycinBiofilmsStructure–activity relationshipPharmacology (medical)PyrrolespyrrolomycinsGram
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Exploring the anticancer activity and the mechanism of action of pyrrolomycins F obtained by microwave-assisted total synthesis

2023

Pyrrolomycins (PMs) are a family of naturally occurring antibiotic agents, isolated from the fermentation broth of Actinosporangium and Streptomyces species. Pursuing our studies on pyrrolomycins, we performed the total synthesis of the F-series pyrrolomycins (1–4) by microwave-assisted synthesis (MAOS), thus obtaining the title compounds in excellent yields (63–69%). Considering that there is no evidence so far of the anticancer effect of this class of compounds, we investigated PMs for their antiproliferative activity against HCT116 and MCF-7 cancer cell lines. PMs showed anticancer activity at submicromolar level with a minimal effect on normal epithelial cell line (hTERT RPE-1), and the…

PharmacologyOrganic ChemistryDrug DiscoveryPyrrolomycin Anticancer activity Microwave-assisted organic synthesis (MAOS) Vacuoles Tunneling nanotubes (TNTs) FilopodiaGeneral MedicineSettore BIO/06 - Anatomia Comparata E CitologiaSettore CHIM/08 - Chimica FarmaceuticaEuropean Journal of Medicinal Chemistry
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Pharmaceutical potential of synthetic and natural pyrrolomycins

2015

The emergence of antibiotic resistance is currently considered one of the most important global health problem. The continuous onset of multidrug-resistant Gram-positive and Gram-negative bacterial strains limits the clinical efficacy of most of the marketed antibiotics. Therefore, there is an urgent need for new antibiotics. Pyrrolomycins are a class of biologically active compounds that exhibit a broad spectrum of biological activities, including antibacterial, antifungal, anthelmintic, antiproliferative, insecticidal, and acaricidal activities. In this review we focus on the antibacterial activity and antibiofilm activity of pyrrolomycins against Gram-positive and Gram-negative pathogens…

Pyoluteorinantibiotic resistancemedicine.drug_classAntibioticsPharmaceutical ScienceMicrobial Sensitivity TestsReviewPharmacologyAntibiofilm agentpyrrolomycinSettore BIO/19 - Microbiologia GeneraleAnalytical Chemistrylcsh:QD241-441Antibiotic resistancelcsh:Organic chemistryDrug DiscoveryDrug Resistance BacterialMedicineAnimalsHumansPyrrolesClinical efficacyPhysical and Theoretical ChemistrypyrrolomycinspentabromopseudilinLow toxicityBacteriabusiness.industryOrganic ChemistryBiological activityBacterial Infectionsantibiofilm agentsAntimicrobialSettore CHIM/08 - Chimica FarmaceuticaAnti-Bacterial AgentsChemistry (miscellaneous)BiofilmsPentabromopseudilinMolecular MedicinebusinessAntibacterial activity
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Pyrrolomycins as antimicrobial agents. Microwave-assisted organic synthesis and insights into their antimicrobial mechanism of action

2019

Abstract New compounds able to counteract staphylococcal biofilm formation are needed. In this study we investigate the mechanism of action of pyrrolomycins, whose potential as antimicrobial agents has been demonstrated. We performed a new efficient and easy method to use microwave organic synthesis suitable for obtaining pyrrolomycins in good yields and in suitable amount for their in vitro in-depth investigation. We evaluate the inhibitory activity towards Sortase A (SrtA), a transpeptidase responsible for covalent anchoring in Gram-positive peptidoglycan of many surface proteins involved in adhesion and in biofilm formation. All compounds show a good inhibitory activity toward SrtA, havi…

Staphylococcus aureusClinical BiochemistryPharmaceutical ScienceMicrobial Sensitivity Testsmedicine.disease_causeSettore BIO/19 - Microbiologia Generale01 natural sciencesBiochemistrychemistry.chemical_compoundBacterial ProteinsDrug DiscoverymedicinePyrrolesEnzyme InhibitorsMicrowavesMolecular BiologyEnzyme Assays010405 organic chemistryChemistryOrganic ChemistryBiofilmN-Acetylmuramoyl-L-alanine AmidaseAntimicrobialAminoacyltransferasesAntimicrobial resistance Pyrrolomycins Sortase A Staphylococcus aureus In-silico docking studies MAOS Pharmacokinetics studies Murein hydrolase activitySettore CHIM/08 - Chimica Farmaceutica0104 chemical sciencesAnti-Bacterial AgentsMolecular Docking Simulation010404 medicinal & biomolecular chemistryCysteine EndopeptidasesBiochemistryMechanism of actionDocking (molecular)Staphylococcus aureusSettore CHIM/03 - Chimica Generale E InorganicaSortase ABiofilmsPseudomonas aeruginosaMolecular MedicineOrganic synthesisPeptidoglycanmedicine.symptom
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Pyrrolomycins as potential anti-staphylococcal biofilms agents

2010

With the goal of discovering new anti-infective agents active against microbial biofilms, this investigation focused on some natural pyrrolomycins, a family of halogenated pyrrole antibiotics. In this study the anti-staphylococcal biofilm activity of pyrrolomycins C, D, F1, F2a, F2b, F3 and of the synthesized related compounds I, II, III were investigated. The susceptibility of six staphylococcal biofilms was determined by methyltiazotetrazolium staining. Most of the compounds were active at concentrations of 1.5 microg ml(-1) with significant inhibition percentages. A few of the compounds were active at the lowest screening concentration of 0.045 microg ml(-1). The population log reduction…

Staphylococcus aureusSynthetic derivativesmedicine.drug_classCell SurvivalAntibioticsPopulationMicrobial Sensitivity TestsAquatic ScienceBiologymedicine.disease_causeSettore BIO/19 - Microbiologia GeneraleApplied Microbiology and BiotechnologyPolymerase Chain ReactionBacterial AdhesionMicrobiologyCell LineInhibitory Concentration 50medicineStaphylococcus epidermidisHumansPyrroleseducationWater Science and TechnologyMicrobial BiofilmsCell Proliferationeducation.field_of_studyMolecular StructureBiofilmStainingAnti-Bacterial AgentsStaphylococcal biofilms Anti-biofilm agents PyrrolomycinsStaphylococcus aureusBiofilmsToxicity
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Novel Sortase A Inhibitors to Counteract Gram-Positive Bacterial Biofilms

2019

Sortase A (SrtA) is a membrane enzyme responsible for the covalent anchoring of surface proteins on the cell wall of Gram-positive bacteria. Nowadays it is considered an interesting target for the development of new anti-infective drugs which aim to interfere with important Gram-positive virulence mechanisms. Along the years, we studied the anti-staphylococcal and anti-biofilm activity of some natural and synthetic polyhalogenated pyrrolic compounds, called pyrrolomycins. Some of them were active on Gram-positive pathogens at a μg/mL range of concentration (1.5-0.045 μg/mL) and showed a biofilm inhibition in the range of 50-80%. [1-3] In light of these encouraging results, herein we present…

chemistry.chemical_classificationbiologyGram-positive virulence mechanismsChemistrySortase ABiofilmlcsh:ASettore BIO/19 - Microbiologia Generalebiology.organism_classificationSettore CHIM/08 - Chimica FarmaceuticaCell wallAnti-infective drugsMembraneEnzymen/aSettore CHIM/03 - Chimica Generale E InorganicaCovalent bondSortase Amental disordersBiophysicslcsh:General WorksAnti-biofilm activityPyrrolomycinsBacteriaGramProceedings
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Biofilm capability of staphylococcus strains isolated from food and the anti-biofilm activity of a chemically synthesized pyrrolomycin

2018


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food isolatespyrrolomycinSettore BIO/19 - Microbiologia GeneraleSettore CHIM/08 - Chimica FarmaceuticaAntibiofilm
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Antibiofilm activity of a novel pyrrolomycin against staphylococcal biofilms of veterinary interest

2011

pyrrolomycin staphylococcal biofilmsSettore BIO/19 - Microbiologia Generale
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