Search results for "rafts"
showing 10 items of 228 documents
The specifics of unmanned aircraft insurance in conjunction with both national and EU rules and procedures
2020
Insurance in many areas of law has been a core matter on both EU and national levels. The currently available research lack or often show no sign of the insurance matters related to Unmanned Aircrafts and other type of aviation objects. This thesis aims to determine what are the core elements of insurance area when discussing Unmanned Aircraft technologies as emerging new component of aviation market. To understand the EU law applicability on MS national legal systems, there arises a question, as to what are the main issues regarding Latvian Unmanned Aircraft legislation concerning insurance for these aircrafts in comparison with the newly developed EU law? In this context, Unmanned Aircraf…
Exosome-associated polysialic acid modulates membrane potentials, membrane thermotropic properties, and raft-dependent interactions between vesicles.
2020
In mammals, polysialic acid (polySia) attached to a small number of transmembrane protein carriers occurs on the surface of plasma membranes of neural, cancer, immune, and placental trophoblast cells. Here, our goal was to demonstrate the presence of polySia on exosomes and its effect on membrane properties. We isolated exosomes and found that polysialylated exosomes in fetal bovine serum originate mostly from placental trophoblasts, while in calf bovine serum, they originate from immune cells. Enzymatic removal of polySia chains from the exosomal surface makes the membrane surface potential more positive, transmembrane potential more negative, and reduces the activation energy for membrane…
Comparative lipidomics and proteomics analysis of platelet lipid rafts using different detergents
2016
Lipid rafts play a pivotal role in physiological functions of platelets. Their isolation using nonionic mild detergents is considered as the gold standard method, but there is no consensual detergent for lipid raft studies. We aimed to investigate which detergent is the most suitable for lipid raft isolation from platelet membrane, based on lipidomics and proteomics analysis. Platelets were obtained from healthy donors. Twelve sucrose fractions were extracted by three different detergents, namely Brij 35, Lubrol WX, and Triton X100, at 0.05% and 1%. After lipidomics analysis and determination of fractions enriched in cholesterol (Ch) and sphingomyelin (SM), proteomics analysis was performed…
Microtomographic reconstruction of mandibular defects treated with xenografts and collagen-based membranes:a pre-clinical minipig model
2021
Background The goal of this study was to evaluate hard tissue response following guided bone regeneration using commercially available bovine bone grafts and collagen membranes; bilayer collagen membrane and porcine pericardium-based membrane, by means of a non-destructive three-dimensional (3D) computerized volumetric analysis following microtomography reconstruction. Material and Methods Bone regenerative properties of various bovine bone graft materials were evaluated in the Göttingen minipig model. Two standardized intraosseous defects (15mm x 8mm x 8mm) were created bilaterally of the mandible of eighteen animals (n=72 defects). Groups were nested within the same subject and randomly d…
A Promising Family of Fluorescent Water-Soluble aza-BODIPY Dyes for in Vivo Molecular Imaging.
2019
A new family of water-soluble and bioconjugatable aza-BODIPY fluorophores was designed and synthesized using a boron- functionalization strategy. These dissymmetric bis-ammonium aza-BODIPY dyes present optimal properties for a fluorescent probe; i.e., they are highly water-soluble, very stable in physiological medium; they do not aggregate in PBS, possess high quantum yield; and finally, they can be easily bioconjugated to antibodies. Preliminary in vitro and in vivo studies were performed for one of these fluorophores to image PD-L1 (Programmed Death-Ligand 1), highlighting the high potential of these new probes for future in vivo optical imaging studies.
A major shift in Late Bronze Age metalwork production around 900 BC
2023
Our traditional chronology systems hardly ever reflect cultural changes accurately and may even mask real moments of discontinuity. This is particularly true for the second part of the Late Bronze Age Urnfield Period where a radical change in iconography can actually be noticed around 900 BC. Within a very short period, the famous solar bird-boat iconography, which had widely developed over centuries and given rise to the elaborate and sophisticated ornamentation known on solid hilted swords from the 11th/10th century, takes a back seat and we can see a single new symbol coming to the fore, concentrating figurative representation in form of a singular vase-shaped build (Wirth S. 2021, 652-6…
Transmission patterns in the production of Bronze Age solid- hilted swords
2023
Bronze Age swords with a bronze hilt were a highly specialised production. Casting hilts and blades and fixing them to each other require lots of precision and very specific skills. We hardly know anything about the production organisation as moulds are extremely rare, preventing us from locating workshops. However, studying swords themselves using X-ray imaging techniques allows us to reconstruct a part of the chaîne opératoire and gain insight into some aspects of the production processes. We identify three periods in the production of solid-hilted swords at the European scale:1. Experimental phase (1800-1450 BC): small-scaled productions by multiple workshops experimenting various ways o…
Production et circulation des épées à poignée métallique de l'âge du Bronze en Europe occidentale
2022
Several regional syntheses have been published on Bronze Age solid-hilted swords, but none for France and the Benelux countries. This dissertation aims first at filling this gap by listing the swords from this region. By adding data from these pre-existing studies, it is possible to study the distribution of solid-hilted swords on a European scale. The building of an inventory is an essential condition to study the diffusion of these weapons over long distances. This must come along with models to characterise the organisation of the swords production and the ways they spread out. It requires us to question the status of the craftsmen, their organisation and their location. One of the first…
Plasma membrane and lysosomal localization of CB1 cannabinoid receptor are dependent on lipid rafts and regulated by anandamide in human breast cance…
2005
AbstractIn this report we show, by confocal analysis of indirect immunofluorescence, that the type-1 cannabinoid receptor (CB1R), which belongs to the family of G-protein-coupled receptors, is expressed on the plasma membrane in human breast cancer MDA-MB-231 cells. However, a substantial proportion of the receptor is present in lysosomes. We found that CB1R is associated with cholesterol- and sphyngolipid-enriched membrane domains (rafts). Cholesterol depletion by methyl-β-cyclodextrin (MCD) treatment strongly reduces the flotation of the protein on the raft-fractions (DRM) of sucrose density gradients suggesting that CB1 raft-association is cholesterol dependent. Interestingly binding of …
CD95 DISC formation and internalizzation occur in lipid rafts of typeI and typeII cells
2004
We investigated the membrane localization of CD95 in type I and type II cells, which differ in their ability to recruit and activate caspase-8. We found that CD95 was preferentially located in lipid rafts of type I cells, while it was present both in raft and non-raft plasma membrane sub-domains of type II cells. After stimulation, CD95 located in phospholipid-rich plasma membrane was recruited to lipid rafts in both types of cells. Similarly, CD95 cross-linking resulted in caspase-independent translocation of FADD/MORT1 and caspase-8 to the lipid rafts, which was prevented by a death domain-defective receptor. CD95 internalization was then rapid in type I and delayed in type II cells and s…