Search results for "ratón"
showing 10 items of 64 documents
Aplicación del binomio Apodemus sylvaticus /helminto como bioindicador de la regeneración del Parc Natural de la Serra Calderona (Comunitat Valencian…
2015
El estudio del binomio Apodemus sylvaticus/helminto ha sido propuesto como un bioindicador del proceso de regeneración postincendio en ecosistemas mediterráneos. El presente trabajo, enmarcado en un proyecto multidisciplinar que lleva por título “Procesos de Recuperación de Ecosistemas Perturbados por el Fuego a través del modelo Helmintos/Micromamíferos” y que fue iniciado por el grupo Parasites & Fire, del Departamento de Parasitología de la Universidad de Farmacia de la Universidad de Valencia, ofrece nuevos resultados que avalan su validación en el Parc Natural de la Serra Calderona (Comunitat Valenciana, España), la cual sufrió un devastador incendio forestal en 1992. Durante 21 años (…
The role of drug sequence in therapeutic selectivity of the combination of 5-fluorouracil and cis-platin.
1989
The therapeutic efficacy of 5-fluorouracil (FUra) and cis-dichlorodiamine-platinum (cis-DDP) in mice bearing transplantable leukemia and solid tumors was evaluated using different sequences of combination of these agents. The optimal sequence was cis-DDP administered 24 h after FUra. The administration of FUra at its maximally tolerated dose (MTD) followed 24 h later by low doses of cis-DDP yielded less toxicity and higher response rate against L1210 and colon 26 than the administration of these two agents in the opposite sequence or concurrently at the MTD. The sequence of administration of these two agents was not therapeutically important when the antitumor activity was evaluated against…
Inflammatory Murine Skin Responses to UV-B Light Are Partially Dependent on Endothelin-1 and Mast Cells
2006
Endothelin (ET-1) has been shown to crucially contribute to UV-induced skin responses such as tanning. To test whether ET-1 is also involved in early cutaneous reactions to UV, we assessed ET-1 skin levels in UV-irradiated mice. In correlation with the levels of UV-induced skin inflammation, ET-1 concentrations increased substantially and continually. Moreover, blocking of ET-1 receptors (ET A ) resulted in significantly decreased cutaneous inflammation following UV irradiation. When we assessed skin responses to ET-1 injections, we observed prominent mast cell degranulation and mast cell-dependent inflammation. Since mast cells also critically contributed to UV-induced inflammation, we det…
Stage of the estrous cycle at the time of pregnant mare's serum gonadotropin injection affects the quality of ovulated oocytes in the mouse
2002
The present study aims to analyze the effect of the stage of the estrous cycle at the time of pregnant mare's serum gonadotropin (PMSG) injection on number and quality of mouse oocytes retrieved from oviducts after exogenous ovarian stimulation. Cellular and morphological traits of ovulated oocytes from hybrid (C57Bl/6JIco female X CBA/JIco male) female mice of 12, 40-42, 50-52 or 57-62 weeks of age were analyzed. Superovulation was induced by a priming injection of PMSG at different stages of the estrous cycle followed after a 48-hr interval by human chrorionic gonadotropin. Injection of PMSG at diestrus-1 was associated with: (1) increased percentage of cumulus-free oocytes; (2) raised to…
Expresión diferencial de proteínas en el íleon de ratón asociadas con el desarrollo de infecciones crónicas por Echinostoma caproni
2015
Entre las enfermedades parasitarias que afectan a la humanidad, las helmintiasis intestinales destacan por su gran morbilidad, ya que actualmente afectan a más de 2000 millones de personas y causan grandes pérdidas económicas relacionadas con la ganadería y la veterinaria. Echinostoma caproni es un trematodo intestinal que, por sus características, es utilizado en numerosos estudios como modelo experimental para determinar los factores por los que se resuelve una infección aguda o se desarrolla una infección crónica causada por helmintos intestinales. En este trabajo se analizó la expresión diferencial de proteínas durante una primoinfección, después de un tratamiento farmacológico y durant…
The designer cytokine hyper-IL-6 mediates growth inhibition and GM-CSF-dependent rejection of B16 melanoma cells.
2000
The low immunogenic B16 melanoma cell line was transfected with a mammalian expression vector containing the complementary DNA for a sIL-6R/IL-6 fusion protein, termed Hyper-IL-6 (H-IL-6), which was shown to have biological activities at 100-1000-fold lower concentrations than IL-6 in combination with sIL-6R. The secreted p84 glycoprotein was detected in the supernatant of transfected cells and was fully active on BAF3/gp130 cells, which respond to IL-6/sIL-6R but not to IL-6 alone. Administration of recombinant H-IL-6 to C57BL/6 mice resulted in a prolonged acute phase protein gene expression indicating long systemic persistence of the fusion protein. Transfected B16 cells (B16/H-IL6 cells…
The addition of interleukin-2 to cyclophosphamide therapy can facilitate tumor growth of B16 melanoma.
1995
The role of interleukin-2 (IL-2) on tumor growth of B16F10 melanoma cells was assessed in two sets of mice with different immune status: normal (immunocompetent) mice and immunodeficient mice. The two sets of animals were treated with cyclophosphamide (CY) or IL-2 alone or with a combined therapy of CY+IL-2. On days 6 and 10 after tumor cell injection, we evaluated the incidence of hepatic B16 melanoma metastases and the percentage of hepatic volume occupied by metastatic tissue. We observed that the CY alone (300 mg/kg, days 3 and 8 post-tumoral inoculation) significantly reduced tumor growth in all treated mice; however, CY proved more effective in normal recipients than in immunodeficien…
Expression of a dominant negative type II TGF-β receptor in mouse skin results in an increase in carcinoma incidence and an acceleration of carcinoma…
1998
The role of Transforming growth factor beta (TGF-beta) in carcinogenesis is complex. There are reports on both tumor inhibition and tumor promotion by TGF-beta. To elucidate the complex role of TGF-beta in epithelial carcinogenesis, we generated transgenic mice overexpressing a dominant negative type II TGF-beta receptor in the basal cell compartment and in follicular cells of the skin. Despite the reduced responsiveness of transgenic keratinocytes to TGF-beta, both proliferation and differentiation were normal in non-irritated epidermis of these transgenic mice. Thus, interruption of signaling of all three isoforms of TGF-beta in basal and follicular cells does not disturb tissue homeostas…
Some thoughts about the conceptual / procedural distinction in translation: a key-logging and eye-tracking study of processing effort
2014
This article builds on the conceptual / procedural distinction postulated by Relevance Theory to investigate processing effort in translation task execution. Drawing on relevance-theoretic assumptions, it assumes that instances related to procedural encodings will require more effortful processing not only in relation to the time spent on the task but also in terms of product indicators such as seconds per word and number of micro translation units per word. Drawing on key-logging and eye-tracking data, the article shows that there are statistically significant differences when conceptual and procedural encodings are analysed in selected areas of interest, with instances related to procedur…
7-Nitroindazole blocks conditioned place preference but not hyperactivity induced by morphine.
2003
The effects of 7-nitroindazole (7-NI), a neural nitric oxide synthase (nNOS) inhibitor, on spontaneous locomotor activity, morphine-induced hyperactivity, acquisition of place conditioning and morphine-induced conditioned place preference (CPP) were evaluated in male mice. In experiment 1, animals treated with 7-NI (25, 50 and 100 mg/kg), morphine (40 mg/kg) or morphine (40 mg/kg) plus 7-NI (25, 50 or 100 mg/kg) were placed in an actimeter for 3 h. In experiment 2, animals treated with the same drugs and doses were conditioned following an unbiased procedure. 7-NI did not affect the spontaneous locomotor activity or hyperactivity induced by morphine. However, the moderate and high doses of …