Search results for "recte"

The fitness effects of synonymous mutations in DNA and RNA viruses.

2011

Despite being silent with respect to protein sequence, synonymous nucleotide substitutions can be targeted by natural selection directly at the DNA or RNA level. However, there has been no systematic assessment of how frequent this type of selection is. Here, we have constructed 53 single random synonymous substitution mutants of the bacteriophages Qb and UX174 by site-directed mutagenesis and assayed their fitness. Analysis of this mutant collection and of previous studies undertaken with a variety of single-stranded (ss) viruses demonstrates that selection at synonymous sites is stronger in RNA viruses than in DNA viruses. We estimate that this type of selection contributes approximately …

Propagation pattern analysis during atrial fibrillation based on sparse modeling.

2012

In this study, sparse modeling is introduced for the estimation of propagation patterns in intracardiac atrial fibrillation (AF) signals. The estimation is based on the partial directed coherence function, derived from fitting a multivariate autoregressive model to the observed signal using least-squares (LS) estimation. The propagation pattern analysis incorporates prior information on sparse coupling as well as the distance between the recording sites. Two optimization methods are employed for estimation of the model parameters, namely, the adaptive group least absolute selection and shrinkage operator (aLASSO), and a novel method named the distance-adaptive group LASSO (dLASSO). Using si…

Editor's Choice – European Society for Vascular Surgery (ESVS) 2021 Clinical Practice Guidelines on the Management of Venous Thrombosis

2021

European Society for Vascular Surgery (ESVS) 2021 Clinical Practice Guidelines on the Management of Venous Thrombosis

Effect of even and odd-order aberrations on the accommodation response

2017

Aim To investigate the potential effect that odd and even-order monochromatic aberrations may have on the accommodation response of the human eye. Methods Eight healthy subjects with astigmatism below 1 D, best corrected visual acuity 20/20 or better and normal findings in an ophthalmic examination were enrolled. An adaptive optics system was used in order to measure the accommodation response of the subjects' eyes under different conditions: with the natural aberrations being present, and with the odd and even-order aberrations being corrected. Three measurements of accommodation response were monocularly acquired at accommodation demands ranging from 0 to 4 D (0.5 D step). Results The acc…

C172S Substitution in the Chloroplast-encoded Large Subunit Affects Stability and Stress-induced Turnover of Ribulose-1,5-bisphosphate Carboxylase/Ox…

1999

Previous work has indicated that the turnover of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1. 39) may be controlled by the redox state of certain cysteine residues. To test this hypothesis, directed mutagenesis and chloroplast transformation were employed to create a C172S substitution in the Rubisco large subunit of the green alga Chlamydomonas reinhardtii. The C172S mutant strain was not substantially different from the wild type with respect to growth rate, and the purified mutant enzyme had a normal circular dichroism spectrum. However, the mutant enzyme was inactivated faster than the wild-type enzyme at 40 and 50 degrees C. In contrast, C172S mutant …

Cysteines 449 and 459 modulate the reduction-oxidation conformational changes of ribulose 1.5-bisphosphate carboxylase/oxygenase and the translocatio…

2006

The role of cysteines 449 (Cys449) and 459 (Cys459) from the large subunit (LS) of ribulose 1-5-bisphosphate carboxylase/oxygenase (Rubisco) in the reduction-oxidation (redox) regulation of the enzyme was assessed by site-directed mutagenesis of these residues and chloroplast transformation of Chlamydomonas reinhardtii. In vitro studies indicated that mutations C449S, C459S or C449S/ C459S do not affect the activity and proteolytic susceptibility of the enzyme in the reduced state. However, when oxidized, the mutant enzymes differed from the wild type (WT), showing an increased resistance to inactivation and, in the case of the double mutant (DM), an altered structural conformation as refle…

Block partial directed coherence: a new tool for the structural analysis of brain Networks

2012

Mediation as a Process for the Management of Conflict and the Improvement of Coexistence in Educational Centres. A Study Based on the Perceptions of …

2017

Abstract School mediation is a strategy which is increasingly being implemented in a greater number of educational centres because of all its benefits. Besides being a communicative process which allows confronting conflicts in a participative manner, it involves learning different social and personal skills. Ultimately, it enables the strengthening of the students’ interpersonal relationships while fostering coexistence at school. If coexistence is built by action and the school is a setting where continuous interactions occur, it seems appropriate to think of the school environment as a place to learn how to coexist. Nevertheless, the studies aimed at knowing the impact of mediation are s…

Expression of C1q, a subcomponent of the rat complement system, is dramatically enhanced in brains of rats with either Borna disease or experimental …

1995

In situ hybridization, RT-PCR and Northern blot analysis as well immunohistochemistry were used to examine the expression of C1q, a subcomponent of the rat complement system, in brains of rats infected with Borna disease virus (BDV) and rats afflicted with experimental allergic encephalomyelitis (EAE) induced by the adoptive transfer of myelin basic protein specific T cells. C1q mRNA, which was not detected in normal brain, became clearly detectable using RT-PCR analysis by d14 post infection (p.i.) with BDV. Maximal levels of C1q mRNA were reached 21 days p.i. when inflammatory reactions in the brain were also at a peak. Similarly, C1q mRNA was elevated when the clinical symptoms of EAE be…

Studentu rakstiskās refleksijas pašizglītošanas veicināšanai angļu valodas stundās 11. klasē

2017

Studentu pašvirzība tiek noteikta pēc tādiem faktiem, kā studentu spēja domāt kritiski, vēlme mācīties patstāvīgi un viņiem piemīt ieradums un tieksme uz pašrefleksiju. Ar pašmācībām tiek pieņemts, ka audzēkņi ir motivēti mācīties un iekarot jaunās virsotnes kāres, izaugsmes nepieciešamības, personīgā gandarījuma no sasniegta un ziņkāres dēļ. Kad skolnieki sāk uzdot jautājumus par viņu mācību procesu, par ieradumiem un mērķiem tajā, viņi kļūst vairāk orientēti uz mērķu sasniegšanu un tādējādi vairāk iesaistīti refleksijas procesā. Refleksijas mērķis ir nodrošināt vairāk atbalsta studentiem, lai tie labāk zinātu viņu stiprās un vājās puses mācībās un tādējādi būtu vairāk pašvirzīti uz sevi m…