Search results for "recte"
showing 10 items of 463 documents
Brīvās rotaļas izmantošana sākumskolas skolēniem pašvadītas mācīšanās sekmēšanai
2022
Diplomdarba nosaukums: “Brīvās rotaļas izmantošana sākumskolas skolēniem pašvadītas mācīšanās sekmēšanai”. Darba mērķis: teorētiski izpētīt un analizēt literatūru, lai empīriski pierādītu brīvās rotaļas izmantošanas nozīmīgumu pašvadītas mācīšanās prasmes attīstīšanai sākumskolas skolēniem. Izstrādāt ieteikumus brīvās rotaļas integrēšanai mācību procesā. Darba uzdevumi: 1.Izpētīt pedagoģijas un psiholoģijas zinātnieku atziņas par pašvadītu mācīšanos, 2.Noskaidrot brīvās rotaļas jēdzienu, veidus, izmantojot pedagoģisko, psiholoģisko, neirobioloģisko, zinātnisko literatūru un analizēt tās ietekmi uz pašvadītu mācīšanos un izmantošanas iespējām sākumskolā. 3.Integrēt brīvās rotaļas stundas māc…
Improving big-data automotive applications performance through adaptive resource allocation
2019
In automotive applications, connected vehicles (CVs) can collect various information (external temperature, speed, location, etc.) and send them to a central infrastructure for exploitation in a wide range of applications: Eco-Driving, fleet management, environmental monitoring, etc. Such applications are known to generate a massive volume of data that is processed in real or near real time (i.e., data streams) depending on the target application requirements. To handle this data volume, big data architectures, based on stream computing paradigm, are usually adopted. Within this paradigm, data are continuously processed by a set of operators (elementary operations) instances. Further, a str…
Stochastic Graph Filtering Under Asymmetric Links in Wireless Sensor Networks
2018
Wireless sensor networks (WSN s) are often characterized by random and asymmetric packet losses due to the wireless medium, leading to network topologies that can be modeled as random, time-varying and directed graphs. Most of existing works related to graph filtering in the context of WSNs assume that the probability of delivering an information from one node to a neighbor node is the same as in the reverse direction. This assumption is not realistic due to the typical link asymmetry in WSNs caused by interferences and background noise. In this work, we analyze the problem of applying stochastic graph filtering over random time-varying asymmetric network topologies. We show that it is poss…
Sport and Quality of Life
2022
Negli ultimi trent’anni lo sport ha assunto un significato molto rilevante nella vita delle persone e, seppure con notevoli differenze, sia nei paesi a sviluppo economico avanzato, sia in quelli in via di sviluppo. A livello individuale, esso ha costituito un’area di investimento identitario quando ha assunto la forma di sport spettacolo del quale fruire, alimentando la pratica del tifo e il fandom o costituendo il modello di uno stile di vita vincente; ma anche quando ha assunto la forma di pratica del tempo libero attraverso la quale tenersi in forma, facendo crescere, in questo caso, la diffusione di una cultura della salute e del benessere. Si tratta di due modalità di approcciare al co…
Engineering the smallest transcription factor: accelerated evolution of a 63-amino acid peptide dual activator-repressor
2019
Transcription factors control gene expression in all life. This raises the question of what is the smallest protein that can support such activity. In nature, Cro from bacteriophage λ is the smallest known repressor (66 amino acids; a.a.) but activators are typically much larger (e.g. λ cI, 237 a.a.). Indeed, previous efforts to engineer a minimal activator from Cro resulted in no activityin vivo. In this study, we show that directed evolution results in a new Cro activator-repressor that functions as efficiently as λ cI,in vivo. To achieve this, we develop Phagemid-Assisted Continuous Evolution: PACEmid. We find that a peptide as small as 63-a.a. functions efficiently as an activator and/o…
PCR-Based Site-Specific Mutagenesis
1998
The alteration of gene structure through the substitution of specific nucleotides by site-specific mutagenesis is an important tool in modern recombinant DNA technology. Nucleotide changes are necessary not only for the analysis of the structural basis of gene and corresponding protein function, but also for the generation of novel gene products. The availability of the polymerase chain reaction (PCR) in the last decade has enabled the modification of DNA for different needs to be made more rapidly and easily than was previously possible. In the course of mutagenesis the relevant sequence changes can be introduced more readily by chemically synthesized oligonucleotide primers than by manipu…
Protein Flexibility and Preorganization in the Design of Enzymes. The Kemp Elimination Catalyzed by HG3.17
2015
A recently designed enzyme, HG3.17, obtained by directed evolution, has shown a catalytic activity close to natural enzymes. Hybrid QM/MM molecular dynamics simulations for the Kemp elimination in this new enzyme have provided a deep insight into the origin of its catalytic efficiency. In this case, we have first demonstrated the presence of different conformations with significantly different reactivity. The larger reactivity is related with a better electrostatic preorganization of the environment that creates a more favorable electrostatic potential for the reaction to proceed. In HG3.17, efforts to improve the catalytic properties must be focused in possible mutations increasing the pre…
Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed m…
2002
In the biosynthesis of the antiarrhythmic alkaloid ajmaline, polyneuridine aldehyde esterase (PNAE) catalyses a central reaction by transforming polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmalane skeleton. The PNAE cDNA was previously heterologously expressed in E. coli. Sequence alignments indicated that PNAE has a 43% identity to a hydroxynitrile lyase from Hevea brasiliensis, which is a member of the α/β hydrolase superfamily. The catalytic triad, which is typical for this family, is conserved. By site-directed mutagenesis, the members of the catalytic triad were identified. For further detection of the active residues, a model…
Discordances entre mémoire et histoire. Un exemple : le congrès d'Amiens (1906)
2012
Memory and history are sometimes a strange couple. A striking illustration is given by the French Unionism in the twentieth century. Some events - 1906, 1920, 1936, 1947, 1968 ... - are engraved in the "memories" and they have founded a collective identity. However, taking the example of the CGT Congress of Amiens in 1906, several dimensions - a priori essential - of this "memory" can be discussed: the importance of the strike, the relationship between parties and unions, the historical weakness of the French unionism. Historical data are not consistent with these representations usually carried about French unionism before 1914. One can wonder : would not it be the same discordance about t…
Cinc tipus d’alliberament laparoscòpic de l’angle esplènic. Lligament pancreatocòlic, un nou lligament en cirurgia colorrectal. Estudi anatomo-quirúr…
2023
INTRODUCCIÓ:La mobilització de l'angle esplènic del còlon (MAEC) és una maniobra necessària a la cirurgia colorectal. El coneixement anatòmic d'aquesta zona quirúrgica és fonamental per evitar complicacions intraoperatòries. S'han descrit tres tipus de MAEC: anterior, lateral i medial. Clàsicament s'han descrit 3 lligaments que fixen angle esplènic del còlon (AEC): gastrocòlic, frenocòlic i esplenocòlic. A més hi ha fixacions entre el cos-cua pancreàtic i la zona adjacent del mesocòlon transvers que no reben un nom específic. Els objectius d’aquest treball són: demostrar l’existència de cinc formes diferents de MAEC per via laparoscòpica; i descriure, des del punt de vista macro i microscòp…