Search results for "response"
showing 10 items of 4136 documents
Unexpected Modulation of Recall B and T Cell Responses after Immunization with Rotavirus-like Particles in the Presence of LT-R192G
2010
LT-R192G, a mutant of the thermolabile enterotoxin of E. coli, is a potent adjuvant of immunization. Immune responses are generally analyzed at the end of protocols including at least 2 administrations, but rarely after a prime. To investigate this point, we compared B and T cell responses in mice after one and two intrarectal immunizations with 2/6 rotavirus-like particles (2/6-VLP) and LT-R192G. After a boost, we found, an unexpected lower B cell expansion measured by flow cytometry, despite a secondary antibody response. We then analyzed CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) and CD4(+)CD25(+)Foxp3(-) helper T cells after in vitro (re)stimulation of mesenteric lymph node cells …
Primary in vivo T cell reactivity of NZB grafts in H-2 identical allogenic hosts.
1983
By means of the Simonson GVH-assay and the popliteal lymph node (PLN) assay, the T-cell reactivity of NZB mice against H-2 identical allogenic cells was investigated in vivo and compared to that of normal mice. None of the normal mice did react, but a highly significant NZB response could be demonstrated, which did not depend on differences in Mls antigens. These in vivo results extend previous findings of a T-cell hyperreactivity of NZB mice in primary in vitro reactions. They favour the possibility that the T-cell hyperreactivity might be relevant in vivo in facilitating autoimmune responses.
Diacylglycerol-containing oleic acid induces increases in [Ca(2+)](i) via TRPC3/6 channels in human T-cells.
2011
Though most of the studies have focused on the effects of free fatty acids on T-cell activation, fatty acids incorporated into plasma membrane phospholipids may also affect cell signaling via diacylglycerol (DAG), generally produced by phospholipid hydrolysis. In the present study, we have synthesized a DAG-containing oleic acid and studied its implication in the modulation of calcium signaling in human Jurkat T-cells. 1-palmitoyl-2-oleoyl-sn-glycerol (POG) induced a dose-dependent increase in [Ca(2+)](i). This effect was due to the presence of oleic acid at the sn-2 position as no differences were observed between POG and 1-stearoly-2-oleoyl-sn-glycerol (SOG). However, the substitution of …
“MIATA”—Minimal Information about T Cell Assays
2009
Immunotherapy, especially therapeutic vaccination, has a great deal of potential in the treatment of cancer and certain infectious diseases such as HIV (Allison et al., 2006; Fauci et al., 2008; Feldmann and Steinman, 2005). Numerous vaccine candidates have been tested in patients with a variety of tumor types and chronic viral diseases. Often, the best way to assess the clinical potential of these vaccines is to monitor the induced T cell response, and yet there are currently no standards for reporting these results. This letter is an effort to address this problem.
Novel method and instruments for the optimal techno-economic sizing of borehole heat exchangers
2021
El test de respuesta térmica (TRT) es ampliamente utilizado como método estándar para caracterizar las propiedades térmicas del terreno adyacente a un intercambiador de calor enterrado (BHE). Los métodos tradicionales para interpretar los resultados aplican soluciones analíticas o numéricas asumiendo que el terreno es infinito, homogéneo e isotrópico. Sin embargo, en realidad el subsuelo presenta generalmente una estructura estratificada y heterogénea, y por lo tanto las propiedades térmicas pueden variar sustancialmente con la profundidad. En este sentido y con la intención de resolver las limitaciones del TRT estándar, la presente tesis doctoral se centra en el desarrollo de métodos e ins…
Safety and side effects of human and ovine corticotropin-releasing hormone administration in man.
1991
Synthetic human and ovine corticotropin-releasing hormone (hCRH, oCRH) are commonly used as a diagnostic tool of the hypothalamo-pituitary-adrenal axis. In this paper reports about side effects after various modes of CRH-application are analyzed and compared to our corresponding data of human studies with hCRH and oCRH. Generally, CRH is well tolerated after single administration and interval-application of standard doses, although minor side effects appear sometimes after higher doses (greater than 200 micrograms hCRH, oCRH) of CRH-bolus-injections. Predominantly the cardiovascular system (e.g. tachycardia, hypotension, flushing) is affected; neuropsychological symptoms are only seen spora…
Identification and dose dependency of ibuprofen biliary metabolites in rainbow trout
2012
Abstract The biotransformation of the anti-inflammatory drug ibuprofen (IBF) was studied by exposing rainbow trout ( Oncorhynchus mykiss ) to IBF via intraperitoneal (i.p.) injection, and via water at four (0.17, 1.9, 13 and 145 μg L −1 ) exposure levels for 4 d. Following exposure, the bile was collected and analyzed by LC–MS/MS methods. The identification of the formed metabolites in i.p. injected fish bile was based on the exact mass determinations by a time-of-flight mass analyzer (Q–TOF–MS) and on the studies of fragments and fragmentation patterns of precursor ions by ion trap mass analyzer (IT-MS). In addition to unmetabolized IBF, several phase I and phase II metabolites were found …
Epigenetic Regulation of Early- and Late-Response Genes in Acute Pancreatitis
2015
Abstract Chromatin remodeling seems to regulate the patterns of proinflammatory genes. Our aim was to provide new insights into the epigenetic mechanisms that control transcriptional activation of early- and late-response genes in initiation and development of severe acute pancreatitis as a model of acute inflammation. Chromatin changes were studied by chromatin immunoprecipitation analysis, nucleosome positioning, and determination of histone modifications in promoters of proinflammatory genes in vivo in the course of taurocholate-induced necrotizing pancreatitis in rats and in vitro in rat pancreatic AR42J acinar cells stimulated with taurocholate or TNF-α. Here we show that the upregulat…
Activity profile, PlayerLoad™ and heart rate response of Gaelic football players : A pilot study
2019
The objectives of this study were to; quantify positional differences in the activity profiles of Gaelic football players and to evaluate decrements in physical performance during a pre-season competition. Global positioning system (GPS) data was recorded from 36 players from 3 teams across 5 games. The relative distance covered in locomotor activities, peak speed, relative PlayerLoad™ (PL.min -1 )and heart rate responses were evaluated between playing positions and across match periods using a mixed model analysis. The mean relative distance of 92.4 ± 23.3 m.min -1 covered,comprised 28.4 ± 10.2 m.min -1 of high intensity running (m.min -1 ≥4.0 m.s -1 ) and 9.9 ± 3.9 m.min -1 of very high i…
Demand Response Service Certification and Customer Baseline Evaluation Using Blockchain Technology
2020
The use of Distributed Ledger Technologies such as Blockchain for certifying Demand Response services allows for the creation of a distributed system in which customers can communicate with the system operator to provide their flexibility, in a secure, transparent and traceable way. Blockchain technology also supports incentive mechanisms for users taking part in the service through the generation of utility tokens to recognize the user's contribution. This paper presents the experimental test of a novel methodology for Demand Response programs implementation by using the Blockchain technology. The latter is employed for defining a distributed Demand Response service and a new system for it…