Search results for "saccharomyces"
showing 10 items of 861 documents
Engineering a Saccharomyces cerevisiae Wine Yeast That Exhibits Reduced Ethanol Production during Fermentation under Controlled Microoxygenation Cond…
2006
ABSTRACTWe recently showed that expressing an H2O-NADH oxidase inSaccharomyces cerevisiaedrastically reduces the intracellular NADH concentration and substantially alters the distribution of metabolic fluxes in the cell. Although the engineered strain produces a reduced amount of ethanol, a high level of acetaldehyde accumulates early in the process (1 g/liter), impairing growth and fermentation performance. To overcome these undesirable effects, we carried out a comprehensive analysis of the impact of oxygen on the metabolic network of the same NADH oxidase-expressing strain. While reducing the oxygen transfer rate led to a gradual recovery of the growth and fermentation performance, its i…
Rbt1 Protein Domains Analysis in Candida albicans Brings Insights into Hyphal Surface Modifications and Rbt1 Potential Role during Adhesion and Biofi…
2013
Cell wall proteins are central to the virulence of Candida albicans. Hwp1, Hwp2 and Rbt1 form a family of hypha-associated cell surface proteins. Hwp1 and Hwp2 have been involved in adhesion and other virulence traits but Rbt1 is still poorly characterized. To assess the role of Rbt1 in the interaction of C. albicans with biotic and abiotic surfaces independently of its morphological state, heterologous expression and promoter swap strategies were applied. The N-terminal domain with features typical of the Flo11 superfamily was found to be essential for adhesiveness to polystyrene through an increase in cell surface hydrophobicity. A 42 amino acid-long domain localized in the central part o…
Akvaporīnu loma raugu šūnu dehidratācijas procesā
2018
Bakalaura darba mērķis bija izpētīt akvaporīnu lomu raugu šūnās dehidratācijas un sekojošas rehidratācijas laikā. Lai sasniegtu šo mērķi izmantoja sekojošos rauga Saccharomyces cerevisiae celmus: ANT29 - izejas celmu ar AQY1 un AQY2 akvaporīnu gēniem, ANT27 - celms ar AQY1 gēna pārekspresiju, un YSH1172 - celms ar abu akvaporīnu gēnu delēciju, kurus sadarbības ietvaros piegādāja Lisabonas Universitāte. Dehidratācijas eksperimentiem izmantoja šūnas no stacionārās augšanas fāzes, kura iestājās 30. augšanas stundā visiem celmiem līdzīgi. Pētīja raugu rezistenci dehidratācijai atkarīgi no pavadīta laika stacionārajā augšanas fāzē. Iegūtie dati parādīja, ka AQY1 pārekspresija raugu šūnās neietek…
Non-
2017
Nitrogen sources in the must are important for yeast metabolism, growth, and performance, and wine volatile compounds profile. Yeast assimilable nitrogen (YAN) deficiencies in grape must are one of the main causes of stuck and sluggish fermentation. The nitrogen requirement of Saccharomyces cerevisiae metabolism has been described in detail. However, the YAN preferences of non-Saccharomyces yeasts remain unknown despite their increasingly widespread use in winemaking. Furthermore, the impact of nitrogen consumption by non-Saccharomyces yeasts on YAN availability, alcoholic performance and volatile compounds production by S. cerevisiae in sequential fermentation has been little studied. With…
Raugu Saccharomyces cerevisiae žāvēšanas rezistences mehanismu pētījumi
2017
. Tika veikta divu rauga Saccharomyces cerevisiae celmu salīdzināšana - mēreni rezistenta S. cerevisiae 14 un izturīga pret žāvēšanu S. cerevisiae 77 celma, audzēšanai izmantojot dažādus barotņu sastāvus. Ir pētītas rauga šūnu to citoplazmatiskās membrānas funkcionālā stāvokļa izmaiņas pārejot anabiozes stāvoklī. Vēlāk par vispiemērotāko tika izvēlēta YPG barotne. Turpinot eksperimentus rauga biomasa pirms dehidratācijas tika 3 stundas inkubēta dažādos sķīdumos ar augstu osmotisko spiedienu, t.i. laktozes un ksilīta šķīdumi. Tas deva iespēju apskatīt šūnu izdzīvotības palielināšanas spējas un novērtēt izmaiņas šūnu lipīdu uzkrāšanās pēc dehidratācijas. Darbā tika izmantotas vairākas …
The influence of yeast glycosylated proteins on tannins aggregation in model solution
2004
<p style="text-align: justify;">The incidence of glycosylated yeast proteins on tannins aggregation in model solution was investigated using the spectrophotometric method (absorbance 700 nm). Glycosylated proteins released by two commercial <em>Saccharomyces cerevisiae</em> strains (RC212 and BM 45) during alcoholic fermentation in synthetic media, glycosylated proteins extracted by Peat’s method and industrial glycosylated proteins purified and separated by chromatography on Sepharose Concanavalin A were used to visualize effects on tannins aggregation. Results showed that tannins aggregation was limited by the glycosylated proteins according to their origin and their mod…
Casein kinase 2 inhibits HomolD-directed transcription by Rrn7 in Schizosaccharomyces pombe.
2014
In Schizosaccharomyces pombe, ribosomal protein gene (RPG) promoters contain a TATA analogue element called the HomolD box. The HomolD-binding protein Rrn7 forms a complex with the RNA polymerase II machinery. Despite the importance of ribosome biogenesis to cell survival, the mechanisms involved in the regulation of transcription of eukaryotic RPGs are unknown. In this study, we identified Rrn7 as a new substrate of the pleiotropic casein kinase 2 (CK2), which is a regulator of basal transcription. Recombinant Rrn7 from S. pombe, which is often used as a model organism for studying eukaryotic transcription, interacted with CK2 in vitro and in vivo. Furthermore, CK2-mediated phosphorylation…
Investigation of yeast community of “Grillo” grapes and musts from Marsala wine production area.
2009
The oenological interest in the autochthonous yeast applications has increased since they represents an important supplement to wine quality (Martinez et al. 1989; Moreno et al. 1991). Yeast populations harboured onto the surface of berries and in musts of “Grillo” grape variety were isolated and analyzed. In order to obtain a first blastomycetic mapping of Marsala wine production area, eight vineyards were chosen on the basis of different climatic and agronomic parameters, including altitude, exposure,vineyard age, grape biotype, grape cultivation system, vegetative vigour, pruning, green pruning, yield per plant, phytosanity state, irrigation and closeness to wood areas. Analysis of blast…
Growth Pattern of Saccharomyces cerevisiae in Cassava Mill Effluents
2018
Nigeria is the world leading producer of cassava. During processing of gari from cassava tuber large volume of effluents are discharged in the environment which is toxic to the environment and some of its associated biota. This study evaluated the growth pattern of Saccharomyces cerevisiae in cassava mill effluents. The Saccharomyces cerevisiae was isolated from palm wine following standard microbiological procedure. The Saccharomyces cerevisiae was inoculated into the sterile effluents and incubated for 15 days. At every 3days interval, 1ml of the effluents was obtained from the medium and the population density determined. Results of the growth showed that the population of Saccharomyces …
Changes in the Pi uptake and polyP accumulation in Saccharomyces cerevisiae strains deficient in the synthesis of trehalose and/or glycerol
2007
Abstract The intracellular level of free inorganic orthophosphate (P i ) in yeast cells generally depends on the P i uptake capacity, energy state of the cells in respect to the activity of the membrane-associated ATPases and on the activity of metabolic pathways involved in the production of glycerol and trehalose. Batch fermentation was performed to investigate the carbon substrate consumption, the P i uptake capacity and product formation by four Saccharomyces cerevisiae strains differing in their ability to produce glycerol and/or trehalose. The consumption of P i in mutant strains with a lack of the synthesis of the trehalose and/or glycerol exceeded the level for a wild type strain ab…