Search results for "script"
showing 10 items of 5143 documents
Regulation of tartrate metabolism by TtdR and relation to the DcuS–DcuR-regulated C4-dicarboxylate metabolism of Escherichia coli
2009
Escherichia coli catabolizes l-tartrate under anaerobic conditions to oxaloacetate by the use of l-tartrate/succinate antiporter TtdT and l-tartrate dehydratase TtdAB. Subsequently, l-malate is channelled into fumarate respiration and degraded to succinate by the use of fumarase FumB and fumarate reductase FrdABCD. The genes encoding the latter pathway (dcuB, fumB and frdABCD) are transcriptionally activated by the DcuS–DcuR two-component system. Expression of the l-tartrate-specific ttdABT operon encoding TtdAB and TtdT was stimulated by the LysR-type gene regulator TtdR in the presence of l- and meso-tartrate, and repressed by O2 and nitrate. Anaerobic expression required a functional fn…
The Streptomyces coelicolor dnaK operon contains a second promoter driving the expression of the negative regulator hspR at physiological temperature
2006
HspR (heat shock protein regulator) acts as a negative regulator of different genes in many bacteria. In Streptomyces coelicolor hspR gene is part and the transcriptional repressor of the dnaK operon which encodes the DnaK, GrpE, DnaJ chaperone machines and HspR itself. Our experiments led us to the discovery of a second promoter, internal to dnaK operon, located upstream hspR gene. Transcription from this promoter was detected at 30 degrees C indicating that hspR could play a key physiological role.
Characterization of the CtsR stress response regulon in Lactobacillus plantarum.
2010
ABSTRACT Lactobacillus plantarum ctsR was characterized. ctsR was found to be cotranscribed with clpC and induced in response to various abiotic stresses. ctsR deletion conferred a heat-sensitive phenotype with peculiar cell morphological features. The transcriptional pattern of putative CtsR regulon genes was examined in the Δ ctsR mutant. Direct CtsR-dependent regulation was demonstrated by DNA-binding assays using recombinant CtsR and the promoters of the ctsR - clpC operon and hsp1 .
The transcriptional activator rfiA is quorum-sensing regulated by cotranscription with the luxI homolog pcol and is essential for plant virulence in …
2009
The gram-negative phytopathogen Pseudomonas corrugata has an acyl-homoserine lactone (AHL) quorum-sensing (QS) system called PcoI/PcoR that is involved in virulence on tomato. This work identifies, downstream of pcol, a gene designated rfiA, which we demonstrate is directly linked to QS by cotranscription with pcol. The deduced RfiA protein contains a DNA-binding domain characteristic of the LuxR family but lacks the autoinducer-binding terminus characteristic of the QS LuxR-family proteins. We also identified, downstream of rfiA, an operon designated pcoABC, encoding for the three components of a tripartite resistance nodulation-cell-division (RND) transporter system. The expression of pco…
I: Boxplots - die flexible Alternative zum „Antennenbildchen”
2002
Data description for continuous parameters is sometimes only based on means and standard deviations of measurement series, graphical representation only concentrates on corresponding "MSE plots", which provide means and standard deviations or even only mean squared errors. However, this strategy is only correct for normally distributed data. Outliers may seriously bias mean and standard deviations and may therefore lead to wrong clinical conclusions. The present paper suggests the use of medians and quartiles, which - just like their graphical pendant, the nonparametric "box whisker plot" - can be applied much more flexibly.
Make Fitness Fun: Could Novelty Be the Key Determinant for Physical Activity Adherence?
2020
The benefits of physical activity (PA) are well known and are extensively delineated in the scientific literature. Regular participation in PA, or exercise as its subset (structured, preplanned form of PA), is positively associated with numerous physical and psychological health benefits across all population subgroups (i.e., different age groups, gender, ethnicity, and socioeconomic status) (Paterson and Warburton, 2010; Warburton and Bredin, 2017). The current PA guidelines for adults, proposed by the American College for Sports Medicine and American Heart Association (ACSM/AHA), recommend the accumulation of at least 150 min of moderate intensity aerobic PA per week (Nelson et al., 2007)…
AMADEUS-The acoustic neutrino detection test system of the ANTARES deep-sea neutrino telescope
2011
The AMADEUS (ANTARES Modules for the Acoustic Detection Under the Sea) system which is described in this article aims at the investigation of techniques for acoustic detection of neutrinos in the deep sea. It is integrated into the ANTARES neutrino telescope in the Mediterranean Sea. Its acoustic sensors, installed at water depths between 2050 and 2300 m, employ piezo-electric elements for the broad-band recording of signals with frequencies ranging up to 125 kHz. The typical sensitivity of the sensors is around - 145 dB re 1 V/mu Pa (including preamplifier). Completed in May 2008, AMADEUS consists of six "acoustic clusters", each comprising six acoustic sensors that are arranged at distanc…
Omics approaches to study the oral microbiome
2015
Introducción La visión clásica de los microorganismos como agentes infecciosos ha propiciado que se consideren como meros agentes causales de enfermedades. Sin embargo, la larga historia de coexistencia entre microorganismos y los seres humanos, ha hecho que ambos se hayan adaptado mutuamente y coevolucionen (Dubos et al. 1965, McFall-Ngai 2002). De hecho, en el cuerpo humano habitan 1014 células bacterianas, un orden de magnitud más que células humanas (Savage 1977). Entre los beneficios que la microbiota aporta, están su contribución a digerir alimentos y a aportar nutrientes, regulación del metabolismo, maduración del sistema inmune, etc. Por ello, hoy en día las enfermedades de origen…
Les actes del mustassaf de Sueca (1571-1578)
1990
L’absència generalitzada de documentació relativa a l’actuació processal diària del mostassaf, oficial de l’organització municipal valenciana, s’explica pel caràcter sumari de les seues decisions i la circumscripció a l’àmbit de l’oralitat, prescrita explícitament per la legislació foral. Tanmateix i de forma excepcional, d’un període breu, els anys 1571 a 1578, i en un lloc rural, Sueca, aquest oficial deixà constància escrita de la seua actuació, cosa que ens permet reconstruir l’estructura del procediment judicial seguit pel mostassaf i la tipologia de les causes dirimides, així com de les penes imposades.
Dual orexin receptor blocker suvorexant attenuates hypercapnic ventilatory augmentation in mice
2022
Suvorexant (Belsomra(R)), a dual orexin receptor antagonist widely used in the treatment of insomnia, inhibits the arousal system in the brain. However, the drug’s ventilatory effects have not been fully explored. This study aims to investigate the expression of orexin receptors in respiratory neurons and the effects of suvorexant on ventilation. Immunohistology of brainstem orexin receptor OX2R expression was performed in adult mice (n = 4) in (1) rostral ventral respiratory group (rVRG) neurons projecting to the phrenic nucleus (PhN) retrogradely labeled by Fluoro-Gold (FG) tracer, (2) neurons immunoreactive for paired like homeobox 2b (Phox2b) in the parafacial respiratory group/retrotra…