Search results for "script"

STAT5b is a key effector of NRG-1/ERBB4-mediated myocardial growth

2023

The growth factor Neuregulin-1 (NRG-1) regulates myocardial growth and is currently under clinical investigation as a treatment for heart failure. Here, we demonstrate in several in vitro and in vivo models that STAT5b mediates NRG-1/EBBB4-stimulated cardiomyocyte growth. Genetic and chemical disruption of the NRG-1/ERBB4 pathway reduces STAT5b activation and transcription of STAT5b target genes Igf1, Myc, and Cdkn1a in murine cardiomyocytes. Loss of Stat5b also ablates NRG-1-induced cardiomyocyte hypertrophy. Dynamin-2 is shown to control the cell surface localization of ERBB4 and chemical inhibition of Dynamin-2 downregulates STAT5b activation and cardiomyocyte hypertrophy. In zebrafish e…

A cross-country configurational approach to international academic mobility: exploring mobility effects on academics’ career progression in EU countr…

2022

This study takes a novel perspective on mobility as career script compliance to explore the factors that might influence how mobile academics in a country perceive the impact of international mobility on their overall academic career progression and job options. We conduct a country-level qualitative comparative analysis on a sample of 24 European Union (EU) countries, based on data from European Commission’s MORE3 indicator tool. We find that these perceptions about the impact are shaped by the dominant patterns of mobility in that country, and the general perception of academics in that particular country that international mobility is rewarded in the institutional promotion schemes. This…

A Poem for All Seasons: Alcuin’s “O vos, est aetas”

2017

There is no complete account of the transmission of Alcuin’s poems, which is apparently quite unpredictable if not random. The circulation of a number of short compositions, which accompany his treatises and his letters, and their circulation together, is so far uncharted. Particularly intriguing is the manuscript tradition of “O vos, est aetas,” a poem in seven distichs, in which young men are encouraged to learn rhetoric and to cultivate virtue. The poem generally occurs in connection with two prose works by Alcuin, the De dialectica (henceforth DD) and the Disputatio de rhetorica et virtutibus (henceforth DR). It may be copied either between the two treatises, before the prologue poem of…

Manuskrypt cechu krawców ze zbiorów Archiwum Państwowego w Opolu. Edycja krytyczna

2016

Anonimowy dokument z Archiwum Państwowego w Opolu włączony do zbioru „Cechy miasta Opola z lat 1470-1934, 123” o sygnaturze 80 jest zabytkiem, który ukazuje dzieje kultury materialnej z zakresu rzemiosła krawców i kostiumologii. Datowany jest on na około 1700 rok i z pewnością powstał na Śląsku. Podstawowym składnikiem opolskiego manuskryptu są wykroje w skali 23 prac krawieckich, głównie ubiorów noszonych przy końcu XVII wieku, ale wśród krawieckich szablonów znalazły się także wzory użytkowe, takie jak chorągiew kościelna, namiot wojskowy, strój na konia, dekoracja elewacji domu. Do unikatu wśród tego zbioru należy zaliczyć wykrój kontusza należącego do kompletu ubiorów polskich. Jego obe…

Transcriptómica en centenarios: un ejemplo de envejecimiento satisfactorio

2021

Los centenarios son un modelo de envejecimiento satisfactorio. No solo disfrutan de una larga longevidad, sino que además muestran una envidiable compresión de la morbilidad. El objetivo del estudio fue esclarecer si las personas centenarias tienen un perfil genético de expresión característico en comparación con personas septuagenarias y jóvenes, así como evaluar sus diferencias a nivel de estrés oxidativo. Para ello se obtuvo muestras de células polimorfonucleares de todos los grupos de edad y se realizó un análisis de su miRNoma y transcriptoma. El análisis de componentes principales reveló un solapamiento entre el perfil de miRNoma de centenarios y el de jóvenes, ambos distintos de los …

Analyse sensorielle du fromage

2018

Mapping of 7-methylguanosine (m7G), 3-methylcytidine (m3C), dihydrouridine (D) and 5-hydroxycytidine (ho5C) RNA modifications by AlkAniline-Seq

2021

Precise and reliable mapping of modified nucleotides in RNA is a challenging task in epitranscriptomics analysis. Only deep sequencing-based methods are able to provide both, a single-nucleotide resolution and sufficient selectivity and sensitivity. A number of protocols employing specific chemical reagents to distinguish modified RNA nucleotides from canonical parental residues have already proven their performance. We developed a deep-sequencing analytical pipeline for simultaneous detection of several modified nucleotides of different nature (methylation, hydroxylation, reduction) in RNA. The AlkAniline-Seq protocol uses intrinsic fragility of the N-glycosidic bond present in certain mod…

Engineering the smallest transcription factor: accelerated evolution of a 63-amino acid peptide dual activator-repressor

2019

Transcription factors control gene expression in all life. This raises the question of what is the smallest protein that can support such activity. In nature, Cro from bacteriophage λ is the smallest known repressor (66 amino acids; a.a.) but activators are typically much larger (e.g. λ cI, 237 a.a.). Indeed, previous efforts to engineer a minimal activator from Cro resulted in no activityin vivo. In this study, we show that directed evolution results in a new Cro activator-repressor that functions as efficiently as λ cI,in vivo. To achieve this, we develop Phagemid-Assisted Continuous Evolution: PACEmid. We find that a peptide as small as 63-a.a. functions efficiently as an activator and/o…

Analysis of pseudouridines and other RNA modifications using hydraPsiSeq protocol

2021

Detection of RNA modified nucleotides using deep sequencing can be performed by several approaches, including antibody-driven enrichment and natural or chemically induced RT signatures. However, only very few RNA modified nucleotides generate natural RT signatures and antibody-driven enrichment heavily depends on the quality of antibodies used and may be highly biased. Thus, the use of chemically-induced RT signatures is now considered as the most trusted experimental approach. In addition, the use of chemical reagents allows inclusion of simple "mock-treated" controls, to exclude spontaneous RT arrests, SNPs and other misincorporation-prone sites. Hydrazine is a well-known RNA-specific rea…

Mapping and Quantification of tRNA 2′-O-Methylation by RiboMethSeq

2018

Current development of epitranscriptomics field requires efficient experimental protocols for precise mapping and quantification of various modified nucleotides in RNA. Despite important advances in the field during the last 10 years, this task is still extremely laborious and time-consuming, even when high-throughput analytical approaches are employed. Moreover, only a very limited subset of RNA modifications can be detected and only rarely be quantified by these powerful techniques. In the past, we developed and successfully applied alkaline fragmentation-based RiboMethSeq approach for mapping and precise quantification of multiple 2'-O-methylation residues in ribosomal RNA. Here we descr…