Search results for "secret"

showing 10 items of 1132 documents

Secretion, interaction and assembly of two O-glycosylated cell wall antigens from Candida albicans.

2001

The mechanisms of incorporation of two antigens have been determined using a monoclonal antibody (3A10) raised against the material released from the mycelial cell wall by zymolyase digestion and retained on a concanavalin A column. One of the hybridomas secreted an IgG that reacted with two bands in Western blots. Indirect immunofluorescence showed that the antigens were located on the surfaces of mycelial cells, but within the cell walls of yeasts. These antigens were detected in a membrane preparation, in the SDS-soluble material and in the material released by a 1,3-beta-glucanase and chitinase from the cell walls of yeast and mycelial cells. In the latter three samples, an additional h…

Antigens FungalGlycosylationbeta-GlucansMicrobiologyCell wallEndoglycosidase Hchemistry.chemical_compoundMiceAntigenChitinCell WallCandida albicansAnimalsSecretionFluorescent Antibody Technique IndirectGlucansAntibodies FungalMice Inbred BALB CMembrane GlycoproteinsbiologyAntibodies MonoclonalMolecular biologyBiochemistrychemistryConcanavalin AChitinasebiology.proteinFemaleAntibodyMicrobiology (Reading, England)
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Sentence arbitrale. Secret du délibéré, Opinion dissidente, Communication aux parties, Conséquence, Le contrôle de la décision d'incompétence des arb…

1995

International audience; (Paris, 7 juill. 1994, Rev. arb. 1995. 106, note S. Jarvin ; Uzinexportimport Romanian Co c/ Attock Cement C°)

Arbitrage international[SHS.DROIT]Humanities and Social Sciences/LawCommunication d'une opinion dissidente[SHS.DROIT] Humanities and Social Sciences/LawARBITRAGESentence arbitraleSecret du délibéré
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La validité de l'opinion dissidente en droit français interne et international de l'arbitrage

2009

International audience; (Paris, 1re ch. C, 9 oct. 2008, n° 07/6619, SAS Merial c/ Sté Klocke Verpackungs - Service Gmbh, inédit)

Arbitrage international[SHS.DROIT]Humanities and Social Sciences/Law[SHS.DROIT] Humanities and Social Sciences/LawOpinion dissidenteARBITRAGESentence arbitraleSecret du délibéré
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Effects of Atropine on Acetylcholine Overflow from Perfused Chicken Hearts

1978

Isolated chicken hearts were perfused (20 ml/min) with Tyrode’s solution. Release of acetylcholine (ACh) was evoked either by electrical stimulation (1 ms; 15 mA) of both preganglionic vagus nerves or by perfusion with dimethylphenylpiperazinium (DMPP). ACh was extracted from the perfusates by ion-pair extraction and determined by gas chromatography.

AtropineChromatographySalivary secretionChemistryDimethylphenylpiperaziniumSmall intestinal motilityExtraction (chemistry)medicineStimulationPerfusionAcetylcholinemedicine.drug
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Absence of muscarinic modulation of vasopressin release from the isolated rat neurohypophysis

1975

1. Isolated rat neurohypophyses were incubated in Locke solution at 37°C and the vasopressin output into the medium determined by bioassay. 2. Potassium chloride 60 mM caused a 9-fold increase in the rate of vasopressin release that was abolished when calcium chloride was omitted from the Locke solution. 3. Acetylcholine 5.5×10−4 M neither alone nor in the presence of atropine 2.9×10−6 M changed the “resting” release of vasopressin. 4. Neither acetylcholine 5.5×10−4 M nor oxotremorine 10−4 and 3×10−4 M altered the vasopressin release evoked by potassium chloride 60 mM. 5. In contrast to the peripheral adrenergic nerve fibres, the secretory terminal fibres of the neurohypophysis do not appea…

Atropinemedicine.medical_specialtyVasopressinVasopressinschemistry.chemical_elementIn Vitro TechniquesCalciumInhibitory postsynaptic potentialPotassium ChloridePituitary Gland PosteriorInternal medicineMuscarinic acetylcholine receptormedicineOxotremorineAnimalsPharmacologyNeurosecretionChemistryOxotremorineGeneral MedicineAcetylcholineRatsAtropineEndocrinologyNicotinic agonistParasympathomimeticsCalciumFemaleAcetylcholinemedicine.drugNaunyn-Schmiedeberg's Archives of Pharmacology
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B7.1 Costimulatory Molecule Is Expressed on Thyroid Follicular Cells in Hashimoto’s Thyroiditis, But Not in Graves’ Disease1

1998

The molecules of the B7 family play a major role in T-lymphocyte costimulation through interaction with their counterreceptors CD28 and CTLA4. In the present study, we analyzed the possible expression of B7 molecules on surgically removed thyroid tissue of patients with autoimmune [Hashimoto's thyroiditis (HT) or Graves' disease (GD)] or nonautoimmune [nontoxic goiter (NTG) or papillary cancer (PC)] thyroid diseases. We found clear positivity of thyroid follicular cells for B7.1 in HT but not in GD, nor in nonautoimmune specimens (NTG, PC) using in situ analysis by alkaline phosphatase anti-alkaline phosphatase (APAAP) technique. Double immunostaining experiments in combination with an anti…

Autoimmune diseaseendocrine systemmedicine.medical_specialtyendocrine system diseasesbusiness.industryEndocrinology Diabetes and MetabolismGraves' diseaseBiochemistry (medical)Clinical BiochemistryThyroidCD28medicine.diseaseBiochemistryFollicular cellThyroiditisEndocrinologymedicine.anatomical_structureEndocrinologyInternal medicineFollicular phasemedicineCytokine secretionbusinessThe Journal of Clinical Endocrinology & Metabolism
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In light of the ends. Copyright hysteresis and private copy exception after the British Academy of Songwriters, Composers and Authors (BASCA) and oth…

2015

In British Academy of Songwriters, Composers and Authors (BASCA) and others v Secretary of State for Business, Innovation and Skills, the High Court of Justice in matter of private copy exception provides the twofold prime opportunity to shed light on the state of the art of copyright in the UK and to flesh out the idea of 'legal hysteresis’. I support the reintroduction of the private copy exception, possibly in a less narrow fashion, and I explain the reasons why I am confident that my expectations will be fulfilled.

BASCA private copy case law hysteresiInformation Society Copyright and Rights in Performance (Personal Copies for Private Use) Regulations exceptions and limitationUK compensation pricing-in British Academy of Songwriters Composers and Authors and others R (On the Application Of) v Secretary of State for Business Innovation and Skills private copy exception levies intellectual property comparative law interpretation of copyright aims of copyrightCopyrightInfosoc DirectiveSettore IUS/01 - Diritto Privato
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Stimulated and unstimulated saliva samples have significantly different bacterial profiles

2018

Epidemiological studies use saliva on a regular basis as a non-invasive and easy-to-take sample, which is assumed to be a microbial representative of the oral cavity ecosystem. However, comparative studies between different kinds of saliva samples normally used in microbial studies are scarce. The aim of the current study was to compare oral microbiota composition between two different saliva samples collected simultaneously: non-stimulated saliva with paper points and stimulated saliva collected after chewing paraffin gum. DNA was extracted from saliva samples of ten individuals, then analyzed by 16S rRNA pyrosequencing to describe bacterial diversity. The results demonstrate significant d…

Bacterial DiseasesMale0301 basic medicineSalivaPhysiologylcsh:MedicineMicrobiologiaPathology and Laboratory MedicineOral cavityDatabase and Informatics Methodsfluids and secretions0302 clinical medicineOral DiseasesCariesMedicine and Health SciencesFood sciencelcsh:ScienceChildMultidisciplinaryGenomicsBody FluidsBacterial PathogensInfectious Diseasesmedicine.anatomical_structureMedical MicrobiologyParaffinFemaleAnatomyPathogensSequence AnalysisResearch ArticleAdolescentBioinformaticsOral MedicineSequence DatabasesMicrobial GenomicsBiologyResearch and Analysis MethodsDental plaqueMicrobiologyBuccal mucosa03 medical and health sciencesOral Microbiotastomatognathic systemTongueGeneticsmedicineHumansEpidemiologiaSalivaMicrobial PathogensBacteriaBocalcsh:ROrganismsBiology and Life SciencesStreptococcus030206 dentistrymedicine.disease16S ribosomal RNAstomatognathic diseasesBiological Databases030104 developmental biologyEstomatologiaPyrosequencinglcsh:QMicrobiomePLOS ONE
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Proteomic analysis of Kveim reagent identifies targets of cellular immunity in sarcoidosis

2017

Background Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4–6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins. Methods Kv extracts were separated by 1D-SDS-PAGE and 2D-DIGE and then underwent mass spectrometric analysis for protein identification. Sarcoidosis and con…

Bacterial DiseasesMaleProteomics0301 basic medicineCellular immunityPhysiologylcsh:MedicineVimentinBiochemistry0302 clinical medicineTandem Mass SpectrometryImmune PhysiologyMedicine and Health SciencesMedicinelcsh:ScienceCITRULLINATED VIMENTINInnate Immune SystemImmunity CellularMultidisciplinarybiologyMYCOBACTERIAL CATALASE-PEROXIDASEPEPTIDESMiddle Aged3. Good healthMultidisciplinary SciencesInfectious DiseasesGranulomaCytokinesScience & Technology - Other TopicsElectrophoresis Polyacrylamide GelFemaleResearch ArticleAdultSarcoidosisGeneral Science & TechnologyInflammatory DiseasesSYSTEMIC SARCOIDOSISImmunologyANTIGENTUBERCULOSISPeripheral blood mononuclear cell03 medical and health sciencesImmune systemRheumatologyAntigenMD MultidisciplinaryVimentinHumansSecretionScience & Technologybusiness.industrylcsh:RBiology and Life SciencesProteinsMolecular DevelopmentTropical Diseasesmedicine.diseaseRHEUMATOID-ARTHRITISCytoskeletal Proteins030104 developmental biology030228 respiratory systemImmune SystemImmunologybiology.proteinT-CELLSIndicators and Reagentslcsh:QCytokine secretionBODIESPhysiological ProcessesbusinessSpleenEx vivoDevelopmental BiologyRCRESPONSES
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Performance of QuantiFERON-TB Gold Plus for detection of latent tuberculosis infection in pregnant women living in a tuberculosis- and HIV-endemic se…

2017

We evaluated the performance of QuantiFERON-TB Gold Plus (QFT-Plus), which includes two Mycobacterium tuberculosis antigen formulations (TB1 and TB2), for detection of latent tuberculosis infection during pregnancy. Eight-hundred-twenty-nine Ethiopian pregnant women (5.9% HIV-positive) were tested with QFT-Plus, with bacteriological sputum analysis performed for women with clinically suspected tuberculosis and HIV-positive women irrespective of clinical presentation. QFT-Plus read-out was categorized according to the conventional cut-off (0.35 IU/ml) for both antigen formulations. In addition, we analysed the distribution of QFT-Plus results within a borderline zone (0.20–0.70 IU/ml), and i…

Bacterial DiseasesRNA virusesPhysiologyMaternal Healthlcsh:MedicineHIV InfectionsPathology and Laboratory MedicineGastroenterologyWhite Blood Cells0302 clinical medicineImmunodeficiency VirusesPregnancyAnimal CellsMedicine and Health SciencesCell Cycle and Cell Division030212 general & internal medicinePregnancy Complications InfectiousYoung adultlcsh:ScienceMultidisciplinarybiologyLatent tuberculosisT CellsObstetrics and GynecologyGestational ageActinobacteriaInfectious DiseasesMedical MicrobiologyCell ProcessesViral PathogensVirusesFemalePathogensCellular Typesmedicine.symptomResearch ArticleAdultmedicine.medical_specialtyTuberculosisImmune CellsImmunologyViral diseasesMicrobiologyMycobacterium tuberculosisInterferon-gammaYoung Adult03 medical and health sciencesAntigenLatent TuberculosisInternal medicineRetrovirusesmedicineHumansTuberculosisMicrobial PathogensSecretionPregnancyBlood CellsBacteriaTuberculin Testbusiness.industryLentiviruslcsh:ROrganismsHIVBiology and Life SciencesMycobacterium tuberculosisCell BiologyTropical Diseasesbiology.organism_classificationmedicine.disease030228 respiratory systemWomen's HealthSputumlcsh:QEthiopiaMitogensPhysiological ProcessesbusinessInterferon-gamma Release TestsPLOS ONE
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