Search results for "sort"

Identification and quantification of microbial populations in activated sludge and anaerobic digestion processes

2014

Eight different phenotypes were studied in an activated sludge process (AeR) and anaerobic digester AnD) in a full-scale wastewater treatment plant by means of fluorescent in situ hybridization (FISH) and automated FISH quantification software. The phenotypes were ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, phosphate-accumulating organisms (PAO), glycogen-accumulating organisms (GAO), sulphate-reducing bacteria (SRB), methanotrophic bacteria and methanogenic archaea. Some findings were unexpected: (a) Presence of PAO, GAO and denitrifiers in the AeR possibly due to unexpected environmental conditions caused by oxygen deficiencies or its ability to survive …

In vitro differentiation of E-N-CAM expressing rat neural precursor cells isolated by FACS during prenatal development

1994

Most fetal rat brain cells expressing the embryonal, highly sialylated form of the cell adhesion molecule N-CAM (E-N-CAM) are precursor cells, as judged from the absence of marker molecules specific for mature neural cell types. However, the detection of E-N-CAM+ cells in frozen sections does not provide information on the lineage-specific differentiation of these cells during development. To investigate their differentiation behaviour in vitro, E-N-CAM+ cells were isolated at different times of brain development by fluorescence-activated cell sorting (FACS), using a monoclonal antibody (Mab RB21-7) which specifically recognizes polysialic acid (PSA) residues on E-N-CAM. Double-immunofluore…

Jaunu Staphylococcus Epidermidis sortāzes A inhibitoru atlase izmantojot FRET skrīninga sistēmu

2020

Pētījuma mērķis ir atrast jaunus zāļu kandidātus, kuri spēj inhibēt Staphylococcus epidermidis sortāzi A, vienlaicīgi mazinot patogēnu multirezistēnces izplatīšanos. Šim mērķim tika izveidoti ģenētiski DNS konstrukti, kuri kodē S.epidermidis sortāzes A katalītisko domēnu, iebūvējot pET22b un pET28a vektoros srtA gēnu. Rekombinantā sortāzes proteīna sintēze tika inducēta ar IPTG, izmantojot BL21(DE3) celmu Escherichia coli ekspresijas sistēmā. Proteīns bez papildus heksahistidīna sekvences (pET22b vektors) tika attīrīts ar anjonapmaiņas hromatogrāfijas kolonnām, savukārt heksahistidīna sekvenci saturošā sortāze (pET28a vektors) tika attīrīta iz…

Discovery of a New Class of Sortase A Transpeptidase Inhibitors to Tackle Gram-positive Pathogens: 2-Phenylhydrazonoalkanoic Acid Derivatives

2015

There is an urgent need of anti-virulence agents effective in the prevention or eradication of biofilms that are intrinsically resistant to conventional antibiotics. If we consider that the first step of staphylococcal pathogenesis and of biofilm formation is the bacterial adhesion, promoted by the surface exposed proteins at the cell wall, we believe that new anti-virulence agents could be developed by using as a target the Sortase A (SrtA), the enzyme responsible of linking surface exposed proteins to peptidoglycan. Therefore, SrtA inhibitors could act as anti-adhesion agents useful to prevent Gram positive virulence mechanisms as well as a virulence mechanism based on biofilm formation. …

Aikamme taakka

2013

Choose as many as you wish: Consumer satisfaction and purchase rate increase when choice from large assortments is flexible as opposed to constrained

2020

Five studies across a range of domains show that consumers who can choose as many alternatives as they wish (“flexible choice”), report more positive affective states and purchase more than those who have to choose a pre-defined quantity of products (“constrained” choice). The benefits of choice “flexibility” are stronger in large than small assortments, and are replicated in field and laboratory settings: when people chose cookies after a meal in a restaurant, possible dating partners on a simulated dating website, energy bars from descriptions, and soaps for personal use. The findings have theoretical implications for advancing choice-overload research, as well as practical implications f…

Streptococcus pneumoniae sortāzes A inhibitoru atlase multirezistentu patogēnu izraisīto infekcijas slimību terapijai

2019

Darba mērķis ir atrast jaunas novatoriskas vielas, kas inhibē Grampozitīvās baktērijas Streptococcus pneumoniae transpeptidāzi sortāzi A. Šo inhibitoru atlasei tika izmantota rekombinanta S.pneumoniae sortāzes A katalītisko domēnu kodējoša konstrukcija bez N terminālā reģiona. Šāda rekombinanta transpeptidāze tika ekspresēta E.coli ekspresijas sistēmā un sintezētais proteīns izrādījās šķīstošs. Pēc attīrīšanas sortāze tika izmantota inhibitoru atlasei, izmantojot skrīningu, kas balstās uz fluorescences rezonanses enerģijas pārnesi (FRET). Pavisam tika pārbaudīti 966 potenciāli inhibitori savienojumi, no kuriem tālākām pārbaudēm tika atlasīti trīs labākie, kuri inhibēja IC50 testā izmantotās…

Fortuna in Seneca tragico ed Herc. Oet. 104-5

1998

Contro l'interpretazione di Friedrich,secondo il quale ire deve intendersi nel senso di esse che nel corpus tragico senecano costituirebbe un unicum semantico e comproverebbe quindi la non autenticità dell'Hercules Oetaeus, ritengo la presenza del verbo di movimento indispensabile sulla base dei vv.344 sg. ire ire ad umbras Herculis nuptam libet,/sed non inultam, dove l'esplicitazione del luogo in cui la regina vuole "recarsi" si traduce in chiaro indizio della libido moriendi manifestata precedentemente.

Reginalitat baixmedieval. La significació política, econòmica i cerimonial de la reina consort a la Corona d’Aragó durant els segles XIV i XV

2020

Tradicionalment, s'ha entés que les dones de l'elit medieval no havien tingut cap tipus de significació social, política ni econòmica. La societat medieval havia desenvolupat un sostre que no seria de vidre sinó de ferro que abans de doblegar-se permetent l’accés de les dones a les esferes de poder, es trencaria. Segons aquesta imatge, la participació de les dones en situacions com la pràctica política es limitava a uns pocs casos que semblaven ser l’excepció que confirmava que les dones no havien tingut mai accés al poder. Cadascun d’aquests casos puntuals havia d’entendre’s com una excepcionalitat, una rara avis que havia sobrevolat el panorama polític i havia aconseguit poder per les seu…

Novel Sortase A (SrtA) inhibitors interfere with the formation of staphylococcal biofilms

2013

Staphylococcus aureus, due to its wide arsenal of virulence factors, is a very versatile pathogen responsible for a wide variety of infectious diseases. The virulence factors include the cell-wall associated proteins that have a direct role in the first stage of pathogenesis. The Sortase A (SrtA) transpeptidase is responsible for covalent anchoring to the cell wall of various surface proteins and it is considered a good target to design new antivirulence agents. In this study, we report the identification of an inhibitor of SrtA afforded from the random screening of a small molecular library of around 150 synthetic compounds, through a high throughput assay by using the standard Dabcyl-QALP…