Search results for "spacer"
showing 10 items of 247 documents
Amplified fragment length polymorphisms and sequence data in the phylogenetic analysis of polyploids: multiple origins of Veronica cymbalaria (Planta…
2007
Summary • The origin of polyploid Veronica cymbalaria (Plantaginaceae) was investigated using DNA sequence data and amplified fragment length polymorphism (AFLP) fingerprints to reveal the parentage of this taxon. The use of AFLP fingerprints in phylogenetic analysis is problematic and various methods have therefore been compared. • DNA sequence data (for the internal transcribed spacer (ITS) region and the plastid trnL-F region (trnL intron, 3’exon, and trnL-F spacer)) and polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis of the ITS region suggested a reliable hypothesis for the evolution of the V. cymbalaria complex. This hypothesis allowed evaluation …
Molecular characterisation of the species of the genus Zygosaccharomyces.
2003
The restriction fragments polymorphisms of the mitochondrial DNA and the PCR fragment that comprised the internal transcribes spacers and the 5.8S rRNA gene, together with the electrophoretic karyotypes of 40 strains from the 10 species of the genus Zygosaccharomyces, including the new species Z. lentus were examined. The RFLP's of the ITS-5.8S region showed a specific restriction pattern for each species, including the new species Z. lentus. The only exception were the species Z. cidri and Z. fermentati that produced identical restriction profiles. The electrophoretic chromosome patterns confirmed the differences between the species of this genus, including the phylogenetic closest species…
Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers
1999
The identification and classification of yeasts have traditionally been based on morphological, physiological and biochemical traits. Various kits have been developed as rapid systems for yeast identification, but mostly for clinical diagnosis. In recent years, different molecular biology techniques have been developed for yeast identification, but there is no available database to identify a large number of species. In the present study, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene were used to identify a total of 132 yeast species belonging to 25 different genera, including teleomorphic and anamorphic a…
Accacoelium contortum (Trematoda: Accacoeliidae) a trematode living as a monogenean: morphological and pathological implications
2015
Background Accacoelium contortum (Rudolphi, 1819) Monticelli, 1893 is a frequent but poorly known trematode found on gills, pharynx and digestive tract of the ocean sunfish Mola mola (L.). Although the morphology of A. contortum agrees with that of a typical endoparasitic trematode, with two relatively small suckers and no large holdfasts, this parasite is normally ectoparasitic. The main objective of this paper is to explore this peculiar host-parasite relationship. Methods A total of 106 ocean sunfish were examined for the presence of A. contortum. The oropharyngeal chamber (gills and pharynx) and the digestive tract were analysed. As the previous descriptions of this species seem to be b…
Flavobacterium columnare colony types: connection to adhesion and virulence?
2008
Four different colony morphologies were produced by Flavobacterium columnare strains on Shieh agar plate cultures: rhizoid and flat (type 1), non-rhizoid and hard (type 2), round and soft (type 3), and irregularly shaped and soft (type 4). Colonies produced on AO agar differed from these to some extent. The colony types formed on Shieh agar were studied according to molecular characteristics [Amplified Fragment Length Polymorphism (AFLP), Automated Ribosomal Intergenic Spacer Analysis (ARISA), and whole cell protein SDS-PAGE profiles], virulence on rainbow trout fingerlings, and adhesion on polystyrene and fish gills. There were no molecular differences between colony types within one strai…
Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae)
2008
Belbahri, Lassaad et al.
Multiple approaches to identify bacteria in archaeological waterlogged wood
2013
This study was carried out in collaboration with Soprintendenza del Mare (SM) that started, since 2004, to plan and realize underwater archaeological parks, such as in the Sicilian islands of Pantelleria (Gadir), Levanzo (Cala Minnola), Ustica (Falconiera), Panarea (Basiluzzo) and Filicudi (Capo Graziano). In situ conservation, as well as virtual exhibitions of the same topics, can contribute to ensure the protection and best fruition of underwater cultural heritage. The focus of this study was the identification of bacterial colonies in waterlogged wood samples from the rostrum of a excellent workmanship, that is very likely one of the wrecks attributed to Sextus Pompey fleet (36 BC) and d…
Unsteadiness and transition to turbulence in woven spacer filled channels for Membrane Distillation
2017
To characterize the performance of Membrane Distillation (MD) modules, channels filled with woven spacers were investigated by Computational Fluid Dynamics (including Direct Numerical Simulations and the use of the SST k-Ï turbulence model) and by parallel experiments with Thermochromic Liquid Crystals. The cases considered here regard mutually orthogonal filaments with a spacer pitch to channel height ratio P/H=2, two spacer orientations θ with respect to the main flow (0° and 45°), and bulk Reynolds numbers Re from â¼200 to â¼2000, an interval of great interest in practical MD applications. For both values of θ, CFD predicted steady-state flow for Re up to â¼300, and chaotic flow …
Experimental investigation of two-side heat transfer in spacer-filled channels
2020
Abstract In Membrane Distillation (MD), spacers support the membranes and promote mixing, thus reducing temperature polarization. Their efficient design requires a knowledge of the distribution of the local heat transfer coefficient h and of its dependence on Reynolds number, spacer geometry and flow-spacer relative orientation. In previous work, we applied Thermochromic Liquid Crystals (TLC) and digital image processing to the measurement of h distributions for different spacer configurations; data were used to validate CFD simulations and select turbulence models. For constructive reasons, the test section allowed only one-side heat transfer, while in most MD configurations (e.g. spiral-w…
Characterization of Gibberella fujikuroi complex isolates by fumonisin B-1 and B-2 analysis and by RAPD and restriction analysis of PCR-amplified int…
2000
Summary Twenty nine isolates of Fusarium spp. (twenty four of them belonging to the Gibberella fujikuroi complex) isolated from banana and corn from different geographical regions were analyzed for their ability to produce fumonisins B 1 and B 2 and for genetic relatedness using random amplified polymorphic DNA (RAPD) and restriction analysis of PCR amplification products of the 5.8s ribosomal DNA-intervening internal transcribed spacer regions (ITS I-5.8S-ITS II). For RAPD analysis, six of twenty oligonucleotide primers were selected after testing with five Fusarium spp. isolates and used to characterize 24 additional isolates. DNA fragments from the 29 isolates of Fusarium spp., which wer…