Search results for "specificity"
showing 10 items of 2234 documents
Bermanella marisrubri gen. nov., sp. nov., a genome-sequenced gammaproteobacterium from the Red Sea
2009
5 pages, 2 figures, 1 table
Natural co-occurrence of ochratoxin A, ochratoxin B and aflatoxins in Sicilian red wines
2015
The natural occurrence of ochratoxin A, ochratoxin B, aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 (OTA, OTB, AFB1, AFB2, AFG1, AFG2) in red wines was investigated by HPLC/FLD after immunoaffinity column clean-up in 57 market samples produced in Sicily (Italy). The results showed a very low incidence of these mycotoxins in analysed samples, confirming the high degree of quality and safety of Sicilian red wines. The results indicated 71.9% and 64.9% positive samples for OTA and OTB respectively, with an average level of 0.13 μg l(-1), well below the European maximum permitted levels (MLs). The aflatoxin most frequently detected in the samples was AFG1, present in 57.9% of sample…
Simultaneous detection of the main black aspergilli responsible for ochratoxin A (OTA) contamination in grapes by multiplex real-time polymerase chai…
2009
9 pages.
Rapid determination of ochratoxin A in cereals and cereal products by liquid chromatography.
2004
A new method based on extraction with octylsilica (C8) followed by liquid chromatography coupled with fluorescence detection (LC-FLD) was studied to determine ochratoxin A (OTA) from cereals and cereal products. Optimization of different parameters, such as type and amount of solid phase, type and volume of eluent and amount of sample were carried out. Recovery of OTA from rice samples spiked at 10 ng/g level was of 86% with relative standard deviation of 5%. The limits of detection and quantification of the proposed method were 0.25 and 0.75 ng/g, respectively. Furthermore, LC-FLD after of OTA methylation and liquid chromatography coupled to mass spectrometry with an electrospray interface…
Mycobiota and toxigenic Penicillium species on two Spanish dry-cured ham manufacturing plants
2013
The present study reports the natural mycobiota occurring in dry-cured hams, and in particular on the incidence of mycotoxin-producing fungi. A total of 338 fungal colonies were isolated from three stages of production, these being the post-salting, ripening and aging stages in two manufacturing plants. The results show that fungi were more frequently isolated from the aging stage and that the predominant filamentous fungal genus isolated was Penicillium. Seventy-four of the 338 fungal strains were selected for identification at the species level by using morphological criteria and internal transcribed spacers sequencing. Of the 74 fungal strains, 59 were Penicillium strains. Sixteen Penici…
Real-time PCR based procedures for detection and quantification of Aspergillus carbonarius in wine grapes
2008
9 pages, 4 tables, 1 figure.
A polyphasic approach to the identification of ochratoxin A-producing black Aspergillus isolates from vineyards in Sicily.
2008
Aspergillus strains belonging to section Nigri isolated during a two year survey in eight Sicilian vineyards located on the slopes of Mount Etna (Sicily, Italy) were analysed analyzed in order to characterize species responsible for ochratoxin A (OTA) contamination of grapes. The polyphasic approach permitted analysis of biodiversity of Aspergillus isolates in relation to their morphology, ochratoxigenicity and genetic variability. We assessed OTA production by A. carbonarius, A. niger, A. tubingensis and A. japonicus using an enzyme-linked immunosorbent assay. A. carbonarius isolates were the strongest OTA producers. A subset of 66 representative strains was selected for further DNA-based …
Synthesis and evaluation of fluorine-18 labeled glyburide analogs as β-cell imaging agents
2003
Glyburide is a prescribed hypoglycemic drug for the treatment of type 2 diabetic patients. We have synthesized two of its analogs, namely N-[4-[beta-(2-(2'-fluoroethoxy)-5-chlorobenzenecarboxamido)ethyl]benzenesulfonyl]-N'-cyclohexylurea (2-fluoroethoxyglyburide, 8b) and N-[4-[beta-(2-(2'-fluoroethoxy)-5-iodobenzenecarboxamido)ethyl]benzenesulfonyl]-N'-cyclohexylurea (2-fluoroethoxy-5-deschloro-5-iodoglyburide, 8a), and their fluorine-18 labeled analogs as beta-cell imaging agents. Both F-18 labeled compound 8a and compound 8b were synthesized by alkylation of the corresponding multistep synthesized hydroxy precursor 4a and 4b with 2-[(18)F]fluoroethyl tosylate in DMSO at 120 degrees C for …
The Afrotropical genus Rhinolaetia Schouteden, 1965 and its systematic position within Scutelleridae (Hemiptera: Heteroptera)
2016
The monotypic Afrotropical genus Rhinolaetia Schouteden, 1865 (Hemiptera: Heteroptera: Scutelleridae) is redescribed. The head, wings, female genitalia and habitus of Rhinolaetia overlaeti Schouteden, 1965 are illustrated. Morphological features of Rhinolaetia and selected representatives of six scutellerid subfamilies are listed and compared. The systematic position of this genus is briefly discussed. Close affinity of Rhinolaetia overlaeti with representatives of subfamilies Odontotarsinae and Odontoscelinae is observed.
Self-phase modulation-based 2R regenerator including pulse compression and offset filtering for 42.6 Gbit/s RZ-33% transmission systems
2009
International audience; We report on the experimental and theoretical study of a self-phase-modulation-based regenerator at 42.6 Gbit/s with a return-to-zero 33% format. We point out some detrimental effects such as intrachannel interactions and Brillouin scattering. An efficient solution, relying on a self-phase-modulation-based pulse compressor in combination with the regenerator, is proposed to overcome these detrimental phenomena. The experimental demonstration shows the effectiveness of a wavelength-transparent regenerator at 42.6 Gbit/s with a sensitivity-improvement of more than 5 dB and an eye-opening improvement of 2.3 dB in a back-to-back configuration, as well as a 10 times maxim…